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Antagonistic activity and mechanism of an isolated Streptomyces corchorusii stain AUH-1 against phytopathogenic fungi

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Abstract

The various diseases that occur during the growth of plants usually cause a significant reduction in production and quality of agricultural products. Actinomycetes, especially Streptomyces spp., become a valuable biological control resource due to their preponderant abilities to produce various secondary metabolites with novel structure and remarkable biological activity. The present work aimed to isolate an effective antagonistic actinomycete against various soilborne phytopathogenic fungi. By dual culture with Fusarium oxysporum f. sp. niveum, an antagonistic actinomycete named Streptomyces corchorusii stain AUH-1 was screened out from 26 soil samples. The in vitro bioassay results showed that S. corchorusii stain AUH-1 had a broad-spectrum antagonistic activity against a range of fungal plant pathogens, such as F. oxysporum f. sp. niveum, Phytophthora parasitica var. nicotianae, Rhizoctonia solani, P. capsica, Botryosphaeria dothidea, F. oxysporum f. sp. vasinfectum, Verticillium dahliae, and F. oxysporum f. sp. cucumerinum. According to the morphological observations in scanning electron microscopy (SEM) and fluorescence microscope (FM), it was found that the cell membranes of F. oxysporum f. sp. niveum were damaged when treated with the antifungal metabolite form S. corchorusii stain AUH-1. Meanwhile, the dropped ergosterol formation and increased malondialdehyde levels further confirmed that S. corchorusii strain AUH-1 exerted its antagonistic activity against F. oxysporum f. sp. niveum via damaging the structure and function of cell membranes. In conclusion, S. corchorusii strain AUH-1 showed a promising prospect for the development of biological agent, especially due to its broad-spectrum and effective antagonist on various soil-borne plant pathogens.

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Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (Grant No. 31760546), and the Training Program for Young Scientists of Jiangxi Provincial Department of Science and Technology (20142BCB23025).

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  1. Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources, Jiangxi Agricultural University, Nanchang, 330045, China

    Yong Yang, Shuai-wen Zhang & Kun-tai Li

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  1. Yong Yang
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  2. Shuai-wen Zhang
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  3. Kun-tai Li
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Correspondence to Kun-tai Li.

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Yang, Y., Zhang, Sw. & Li, Kt. Antagonistic activity and mechanism of an isolated Streptomyces corchorusii stain AUH-1 against phytopathogenic fungi. World J Microbiol Biotechnol 35, 145 (2019). https://doi.org/10.1007/s11274-019-2720-z

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