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Agrobacterium-mediated transformation of the ascomycete mushroom Morchella importuna using polyubiquitin and glyceraldehyde-3-phosphate dehydrogenase promoter-based binary vectors

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Abstract

Morchella importuna is a worldwide distributed edible mushroom with high ecological and economic values, but the molecular and genetic research about this mushroom has been hindered due to lack of an efficient transformation method. Here, we report for the first time the successful transformation of M. importuna by using a hypervirulent Agrobacterium tumefaciens strain bearing the constructed binary plasmid p1391-U-GUS. The selectable markers used were the genes for hygromycin resistance under the control of the polyubquitin promoter from M. importuna. The reporter genes were those for enhanced green fluorescent protein (EGFP) and the β-Glucuronidase (GUS) under the control of glyceraldehyde-3-phosphate dehydrogenase promoter and polyubquitin promoter respectively. The presence of the reporter gene EGFP in the transformants was confirmed by the fluorescence and confocal microscope and molecular analysis and that of the reporter gene GUS was verified by enzyme activity and molecular analysis. The analysis results of both reporter genes indicated that Agrobacterium-mediated transformation was successfully performed in M. importuna.

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Acknowledgements

This work was supported by the Fundamental Research Funds for the Central Universities (Grant Number 2662015PY058); Huazhong Agricultural University Scientific & Technological Self-innovation Foundation (Grant Number 2662014BQ016).

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Authors and Affiliations

  1. Institute of Applied Mycology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China

    Shouyun Lv, Xin Chen, Chunye Mou, Shenghong Dai, Yinbing Bian & Heng Kang

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  1. Shouyun Lv
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  2. Xin Chen
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  3. Chunye Mou
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  4. Shenghong Dai
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  5. Yinbing Bian
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  6. Heng Kang
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Correspondence to Heng Kang.

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Lv, S., Chen, X., Mou, C. et al. Agrobacterium-mediated transformation of the ascomycete mushroom Morchella importuna using polyubiquitin and glyceraldehyde-3-phosphate dehydrogenase promoter-based binary vectors. World J Microbiol Biotechnol 34, 148 (2018). https://doi.org/10.1007/s11274-018-2529-1

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