Characterization of bacterial composition and diversity in a long-term petroleum contaminated soil and isolation of high-efficiency alkane-degrading strains using an improved medium

  • Jun Zheng
  • Jun-Qiao Feng
  • Lei Zhou
  • Serge Maurice Mbadinga
  • Ji-Dong Gu
  • Bo-Zhong Mu
Original Paper

Abstract

Bacterial community and diversity in a long-term petroleum-contaminated soil of an oilfield were characterized using 16S rRNA gene-based Illumina MiSeq high-throughput sequencing. Results indicated that Proteobacteria (49.11%) and Actinobacteria (24.24%) were the most dominant phyla, and the most abundant genera were Pseudoxanthomonas (8.47%), Luteimonas (3.64%), Alkanindiges (9.76%), Acinetobacter (5.26%) and Agromyces (8.56%) in the soil. Meanwhile a series of cultivations were carried out for isolation of alkane degraders from petroleum-contaminated soil with gellan gum and agar as gelling agents. And the isolates were classified by their 16S rRNA genes. Nine of the isolates including Enterobacter, Pseudomonas,Acinetobacter, Rhizobium, Bacillus, Sphingomonas, Paenibacillus, Variovorax and Rhodococcus showed strong biodegradability of alkane mixture (C9–C30) in a wide range of chain-length, which could be potentially applied in enhancement of bioremediation.

Keywords

Microbial community Long-term petroleum-contaminated soil Hydrocarbon degradation Gellan gum Bioremediation 

Notes

Acknowledgements

This work was supported by the National Science Foundation of China (No. 41530318), the Research Foundation of Shanghai (No. 15JC1401400), and the Fundamental Research Funds for the Central Universities of China (No. 222201717017).

Supplementary material

11274_2018_2417_MOESM1_ESM.docx (282 kb)
Supplementary material 1 (DOCX 282 KB)

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Jun Zheng
    • 1
  • Jun-Qiao Feng
    • 1
  • Lei Zhou
    • 1
  • Serge Maurice Mbadinga
    • 1
    • 3
  • Ji-Dong Gu
    • 2
  • Bo-Zhong Mu
    • 1
    • 3
  1. 1.State Key Laboratory of Bioreactor Engineering and Institute of Applied ChemistryEast China University of Science and TechnologyShanghaiPeople’s Republic of China
  2. 2.School of Biological SciencesThe University of Hong KongHong KongPeople’s Republic of China
  3. 3.Shanghai Collaborative Innovation Center for Biomanufacturing TechnologyShanghaiPeople’s Republic of China

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