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Bioactive compounds of Aspergillus terreus—F7, an endophytic fungus from Hyptis suaveolens (L.) Poit

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Abstract

The compounds terrein (1), butyrolactone I (2), and butyrolactone V (3) were isolated from the ethyl acetate extract (EtOAc) of the endophytic fungus Aspergillus terreus—F7 obtained from Hyptis suaveolens (L.) Poit. The extract and the compounds presented schistosomicidal activity against Schistosoma mansoni; at 100 µg/mL for EtOAc extract, 1297.3 µM for compound 1, 235.6 µM for compound 2, and 454.1 µM for compound 3, they killed 100% of the parasites after 72 h of treatment. Compounds 1, 2, and 3 exerted moderate leishmanicidal activity against Leishmania amazonensis (IC50 ranged from 23.7 to 78.6 µM). At 235.6 and 227.0 µM, compounds 2 and 3, respectively, scavenged 95.92 and 95.12% of the DPPH radical (2,2-diphenyl-1-picryl-hydrazyl), respectively. Regarding the cytotoxicity against the breast tumor cell lines MDA-MB-231 and MCF-7, compound 2 gave IC50 of 34.4 and 17.4 µM, respectively, while compound 3 afforded IC50 of 22.2 and 31.9 µM, respectively. At 117.6 µM, compound 2 inhibited the growth of and killed the pathogen Escherichia coli (ATCC 25922). Compounds 1, 2, and 3 displayed low toxicity against the normal line of human lung fibroblasts (GM07492A cells), with IC50 of 15.3 × 103, 3.4 × 103, and 5.8 × 103 µM, respectively. This is the first report on (i) the in vitro schistosomicidal and leishmanicidal activities of the EtOAc extract of A. terreus—F7 and compounds 1, 2, and 3; and (ii) the antitumor activity of compounds 2 and 3 against MDA-MB-231 and MCF-7 cells.

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Acknowledgements

The authors thank to the National Council for Scientific and Technological Development (CNPq) (Grant No. 552240/2011-7), Coordination for the Improvement of Higher Education Personnel (CAPES), and National Institute of Science and Technology in Wetlands (INAU) for institutional and financial support.

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Authors and Affiliations

  1. Department of Botany and Ecology, Institute of Biosciences, Federal University of Mato Grosso, Cuiabá, MT, Brazil

    Igor Pereira da Silva, Elson Brissow & Marcos Antônio Soares

  2. Natural Products Research Group, Center for Research in Mathematical Sciences and Technology, University of Franca, Franca, SP, Brazil

    Luis Claudio Kellner Filho, Jaqueline Lopes Damasceno, Suzana Amorim Mendes, Denise Crispim Tavares, Lizandra Guidi Magalhães & Ana Helena Januário

  3. Department of Biological Sciences, University of Santa Cruz, Ilheus, BA, Brazil

    Jaqueline Senabio & Kátia Aparecida de Siqueira

  4. Department of Basic Sciences in Health, School of Medicine, Federal University of Mato Grosso, Cuiabá, MT, Brazil

    Samuel Vandresen Filho

  5. Research Center René Rachou, Oswaldo Cruz Foundation, Belo Horizonte, MG, Brazil

    Policarpo Ademar Sales Junior

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  1. Igor Pereira da Silva
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  2. Elson Brissow
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Correspondence to Marcos Antônio Soares.

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da Silva, I.P., Brissow, E., Kellner Filho, L.C. et al. Bioactive compounds of Aspergillus terreus—F7, an endophytic fungus from Hyptis suaveolens (L.) Poit. World J Microbiol Biotechnol 33, 62 (2017). https://doi.org/10.1007/s11274-017-2228-3

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