Bioactive compounds of Aspergillus terreus—F7, an endophytic fungus from Hyptis suaveolens (L.) Poit

  • Igor Pereira da Silva
  • Elson Brissow
  • Luis Claudio Kellner Filho
  • Jaqueline Senabio
  • Kátia Aparecida de Siqueira
  • Samuel Vandresen Filho
  • Jaqueline Lopes Damasceno
  • Suzana Amorim Mendes
  • Denise Crispim Tavares
  • Lizandra Guidi Magalhães
  • Policarpo Ademar Sales Junior
  • Ana Helena Januário
  • Marcos Antônio Soares
Original Paper

Abstract

The compounds terrein (1), butyrolactone I (2), and butyrolactone V (3) were isolated from the ethyl acetate extract (EtOAc) of the endophytic fungus Aspergillus terreus—F7 obtained from Hyptis suaveolens (L.) Poit. The extract and the compounds presented schistosomicidal activity against Schistosoma mansoni; at 100 µg/mL for EtOAc extract, 1297.3 µM for compound 1, 235.6 µM for compound 2, and 454.1 µM for compound 3, they killed 100% of the parasites after 72 h of treatment. Compounds 1, 2, and 3 exerted moderate leishmanicidal activity against Leishmania amazonensis (IC50 ranged from 23.7 to 78.6 µM). At 235.6 and 227.0 µM, compounds 2 and 3, respectively, scavenged 95.92 and 95.12% of the DPPH radical (2,2-diphenyl-1-picryl-hydrazyl), respectively. Regarding the cytotoxicity against the breast tumor cell lines MDA-MB-231 and MCF-7, compound 2 gave IC50 of 34.4 and 17.4 µM, respectively, while compound 3 afforded IC50 of 22.2 and 31.9 µM, respectively. At 117.6 µM, compound 2 inhibited the growth of and killed the pathogen Escherichia coli (ATCC 25922). Compounds 1, 2, and 3 displayed low toxicity against the normal line of human lung fibroblasts (GM07492A cells), with IC50 of 15.3 × 103, 3.4 × 103, and 5.8 × 103 µM, respectively. This is the first report on (i) the in vitro schistosomicidal and leishmanicidal activities of the EtOAc extract of A. terreus—F7 and compounds 1, 2, and 3; and (ii) the antitumor activity of compounds 2 and 3 against MDA-MB-231 and MCF-7 cells.

Keywords

Antioxidant Antitumor Butyrolactones Leishmanicidal Schistosomicidal Terrein 

Notes

Acknowledgements

The authors thank to the National Council for Scientific and Technological Development (CNPq) (Grant No. 552240/2011-7), Coordination for the Improvement of Higher Education Personnel (CAPES), and National Institute of Science and Technology in Wetlands (INAU) for institutional and financial support.

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Copyright information

© Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  • Igor Pereira da Silva
    • 1
  • Elson Brissow
    • 1
  • Luis Claudio Kellner Filho
    • 2
  • Jaqueline Senabio
    • 3
  • Kátia Aparecida de Siqueira
    • 3
  • Samuel Vandresen Filho
    • 4
  • Jaqueline Lopes Damasceno
    • 2
  • Suzana Amorim Mendes
    • 2
  • Denise Crispim Tavares
    • 2
  • Lizandra Guidi Magalhães
    • 2
  • Policarpo Ademar Sales Junior
    • 5
  • Ana Helena Januário
    • 2
  • Marcos Antônio Soares
    • 1
  1. 1.Department of Botany and Ecology, Institute of BiosciencesFederal University of Mato GrossoCuiabáBrazil
  2. 2.Natural Products Research Group, Center for Research in Mathematical Sciences and TechnologyUniversity of FrancaFrancaBrazil
  3. 3.Department of Biological SciencesUniversity of Santa CruzIlheusBrazil
  4. 4.Department of Basic Sciences in Health, School of MedicineFederal University of Mato GrossoCuiabáBrazil
  5. 5.Research Center René Rachou, Oswaldo Cruz FoundationBelo HorizonteBrazil

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