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α-Bisabolol inhibits Aspergillus fumigatus Af239 growth via affecting microsomal ∆24-sterol methyltransferase as a crucial enzyme in ergosterol biosynthesis pathway

  • Zahra Jahanshiri
  • Masoomeh Shams-Ghahfarokhi
  • Farnoush Asghari-Paskiabi
  • Reza Saghiri
  • Mehdi Razzaghi-Abyaneh
Original Paper
  • 341 Downloads

Abstract

Finding new compounds with antifungal properties is an important task due to the side effects of common antifungal drugs and emerging antifungal resistance in fungal strains. ∆24-sterol methyltransferase (24-SMT) is a crucial enzyme that plays important roles in fungal ergosterol biosynthesis pathway and is not found in humans. In the present study, the effects of α-bisabolol on Aspergillus fumigatus Af239 growth and ergosterol synthesis on the base of 24-SMT enzyme activity were studied; in addition, the expression of erg6, the gene encoded 24-SMT, was considered. To our knowledge, this is the first report demonstrating that α-bisabolol inhibits A. fumigatus growth specifically via suppressing fungal 24-SMT. Since this enzyme is a specific fungal enzyme not reported to exist in mammalian cells, α-bisabolol may serve as a lead compound in the development of new antifungal drugs. Fungi were cultured in presence of serial concentrations of α-bisabolol (0.281–9 mM) for 3 days at 35 °C. Mycelia dry weight was determined as an index of fungal growth and ergosterol content was assessed. Microsomal 24-SMT activity was assayed in presence of α-bisabolol as an inhibitor, lanosterol as a substrate and [methyl-H3] AdoMet (S-Adenosyl methionin). In addition, the expression of erg 6 gene (24-SMT encoding gene) was determined after treatments with various concentrations of α-bisabolol. Our results demonstrated that α-bisabolol strongly inhibited A. fumigatus growth (35.53–77.17%) and ergosterol synthesis (26.31–73.77%) dose-dependently and suppressed the expression of erg 6 gene by 76.14% at the highest concentration of 9 mM. α-bisabolol inhibited the activity of 24-SMT by 99% at the concentration of 5 mM. Taken together, these results provides an evidence for the first time that α-bisabolol inhibits A. fumigatus Af239 growth via affecting microsomal ∆24-sterol methyltransferase as a crucial enzyme in ergosterol biosynthetic pathway.

Keywords

Aspergillus fumigatus α-Bisabolol 24-Sterol methyltransferase Ergosterol biosynthesis Antifungal activity 

Notes

Acknowledgements

This work was financially supported by the Pasteur Institute of Iran (Project No. 647).

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Copyright information

© Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  • Zahra Jahanshiri
    • 1
  • Masoomeh Shams-Ghahfarokhi
    • 2
  • Farnoush Asghari-Paskiabi
    • 1
  • Reza Saghiri
    • 3
  • Mehdi Razzaghi-Abyaneh
    • 1
  1. 1.Department of MycologyPasteur Institute of IranTehranIran
  2. 2.Department of Mycology, Faculty of Medical SciencesTarbiat Modares UniversityTehranIran
  3. 3.Department of BiochemistryPasteur Institute of IranTehranIran

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