World Journal of Microbiology and Biotechnology

, Volume 28, Issue 5, pp 2047–2055

Process optimization of batch biosorption of lead using Lactobacillius bulgaricus in an aqueous phase system using response surface methodology

  • Mehdi Sedighi
  • Mostafa Ghasemi
  • Sedky H. A. Hassan
  • Wan Ramli Wan Daud
  • Manal Ismail
  • Elgorban Abdallah
Original Paper


Response surface methodology (RSM) based on central composite rotatable design was used to investigate the effects of operating variable, mainly, pH, weight of biomass, and initial lead ion concentration on the lead adsorption capacity at ambient temperature using dried cells of Lactobacillius bulgaricus. Using RSM, quadratic polynomial equation was obtained for predicting the percent of lead ion removal. Analysis of variance showed that the effects of pH and weight of dried biomass were concluded to be the key factors influencing the capacity of lead ion removal. At pH lower than 2 (high acidic condition) and in alkaline condition, there is no significant biosorption. The optimum percent of lead ion removal was found at pH of 6.78, biomass concentration of 6.58 g/l and initial lead concentration 36.22 ppm. In this condition, percent of lead ion removal was 86.21%. This study showed RSM effectiveness for modeling of biosorption process.


Lactobacillus bulgaricus Biosorption Lead pH Response surface methodology 


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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Mehdi Sedighi
    • 1
  • Mostafa Ghasemi
    • 2
    • 3
  • Sedky H. A. Hassan
    • 4
  • Wan Ramli Wan Daud
    • 2
    • 3
  • Manal Ismail
    • 2
    • 3
  • Elgorban Abdallah
    • 5
  1. 1.Department of Chemical EngineeringTarbiat Modares UniversityTehranIran
  2. 2.Fuel Cell InstituteUniversity Kebangsaan MalaysiaUKM BangiMalaysia
  3. 3.Department of Chemical and Process Engineering, Faculty of Engineering and Built EnvironmentUniversity Kebangsaan MalaysiaBangiMalaysia
  4. 4.Department of Biological EnvironmentKangwon National UniversityChuncheonSouth Korea
  5. 5.Center of Excellence of Biotechnology ResearchKing Saud UniversityRiyadhSaudi Arabia

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