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Novel anelloviruses identified in buccal swabs of Antarctic fur seals

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Abstract

Viral diversity associated with Antarctic wildlife remains poorly studied. Nonetheless, over the past 5 years, there has been a concerted effort using viral metagenomics approaches to identify and characterize viruses associated with Antarctic pinniped and avian species. Here we used a viral metagenomics approach to identify circular DNA viruses in buccal swab samples from Antarctic fur seals (Arctocephalus gazella) breeding on Livingston Island, Antarctica during the 2016/2017 field season. We identified two new lineages of anelloviruses, torque teno Arctocephalus gazella virus (TTAgV)-1 (2083 nts) and -2 (2127–2143 nts), which are most closely related to and cluster with a previously identified anellovirus associated with California sea lions (Zalophus californianus) sharing ~ 60% genome-wide pairwise identity. The ORF1 of TTAgVs share 26–41% amino acid similarity to the ORF1 of other previously identified pinniped-associated anelloviruses.

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Fig. 1
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Data availability

All sequence data reported in this study has been deposited in GenBank (https://www.ncbi.nlm.nih.gov/genbank/) under Accession #s MG837569–MG837573.

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Acknowledgements

The molecular work described in this study is supported by Center of Evolution and Medicine Venture Fund (Center of Evolution and Medicine, Arizona State University, USA) grant awarded to AS and AV.

Author information

Authors and Affiliations

  1. School of Life Sciences, Arizona State University, Tempe, AZ, 85287-5001, USA

    Adele Crane

  2. Antarctic Ecosystem Research Division, NOAA/NMFS/Southwest Fisheries Science Center, 8901 La Jolla Shores Drive, La Jolla, CA, 92037-1508, USA

    Michael E. Goebel

  3. The Biodesign Center for Fundamental and Applied Microbiomics, Arizona State University, Tempe, AZ, 85287-5001, USA

    Simona Kraberger & Arvind Varsani

  4. School of Human Evolution and Social Change, Arizona State University, Tempe, AZ, 85287-5001, USA

    Anne C. Stone

  5. Center for Evolution and Medicine, Arizona State University, Tempe, AZ, 85287-5001, USA

    Anne C. Stone & Arvind Varsani

  6. Structural Biology Research Unit, Department of Clinical Laboratory Sciences, University of Cape Town, Observatory, Cape Town, South Africa

    Arvind Varsani

Authors
  1. Adele Crane
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  2. Michael E. Goebel
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  4. Anne C. Stone
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  5. Arvind Varsani
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Contributions

MEG collected the samples. AC and SK undertook all the lab work. AV and AC undertook all the bioinformatic analyses. AC, MEG, SK, ACS, and AV wrote the manuscript.

Corresponding author

Correspondence to Arvind Varsani.

Ethics declarations

Conflict of interest

The authors declare that they have no conflicts of interest.

Ethical approval

Antarctic fur seal samples were collected under National Marine Fisheries Service Marine Mammal permit #20599, Antarctic Conservation Act permit #2017-012. The data used for the analyses in this manuscript are publicly available in GenBank.

Additional information

Edited by Zhen F. Fu.

GenBank Accession #: MG837569–MG837573.

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Crane, A., Goebel, M.E., Kraberger, S. et al. Novel anelloviruses identified in buccal swabs of Antarctic fur seals. Virus Genes 54, 719–723 (2018). https://doi.org/10.1007/s11262-018-1585-9

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  • DOI: https://doi.org/10.1007/s11262-018-1585-9

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