Virus Genes

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Gene expression and population polymorphism of maize Iranian mosaic virus in Zea mays, and intracellular localization and interactions of viral N, P, and M proteins in Nicotiana benthamiana

  • Abozar Ghorbani
  • Keramatollah Izadpanah
  • Ralf G. Dietzgen
Article
  • 24 Downloads

Abstract

Maize Iranian mosaic virus (MIMV; Mononegavirales, Rhabdoviridae, Nucleorhabdovirus) infects maize and several other poaceous plants. MIMV encodes six proteins, i.e., nucleocapsid protein (N), polymerase cofactor phosphoprotein (P), putative movement protein (P3), matrix protein (M), glycoprotein (G), and large RNA-dependent RNA polymerase (L). In the present study, MIMV gene expression and genetic polymorphism of an MIMV population in maize were determined. N, P, P3, and M protein genes were more highly expressed than the 5′ terminal G and L genes. Twelve single nucleotide polymorphisms were identified across the genome within a MIMV population in maize from RNA-Seq read data pooled from three infected plants indicating genomic variations of potential importance to evolution of the virus. MIMV N, P, and M proteins that are known to be involved in rhabdovirus replication and transcription were characterized as to their intracellular localization and interactions. N protein accumulated exclusively in the nucleus and interacted with itself and with P protein. P protein accumulated in both the nucleus and cell periphery and interacted with itself, N and M proteins in the nucleus. M protein was localized in the cell periphery and on endomembranes, and interacted with P protein in the nucleus. MIMV proteins show a distinctive combination of intracellular localizations and interactions.

Keywords

Maize rhabdovirus Protein–protein interactions Population polymorphism Viral gene expression 

Notes

Acknowledgements

This research was jointly supported by the Queensland Department of Agriculture and Fisheries and the University of Queensland through the Queensland Alliance for Agriculture and Food Innovation. AG was supported by a scholarship from Shiraz University. Funding for RNA-Seq analysis was provided by Plant Virology Research Center of Shiraz University. We thank Kerry Vinall (University of Queensland) for training AG and expert advice in confocal microscopy.

Author contributions

AG, KI, and RGD designed the study; AG performed the experiments; AG and RGD analyzed the data; AG drafted the manuscript, and all authors edited and approved the final version.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11262_2018_1540_MOESM1_ESM.docx (1.4 mb)
Supplementary material 1 (DOCX 1387 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Abozar Ghorbani
    • 1
    • 2
  • Keramatollah Izadpanah
    • 1
  • Ralf G. Dietzgen
    • 2
  1. 1.College of AgriculturePlant Virology Research Center, Shiraz UniversityShirazIran
  2. 2.Queensland Alliance for Agriculture and Food InnovationThe University of QueenslandSt. LuciaAustralia

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