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Veterinary Research Communications

, Volume 42, Issue 2, pp 95–100 | Cite as

Clinical utility of urine kidney injury molecule-1 (KIM-1) and gamma-glutamyl transferase (GGT) in the diagnosis of canine acute kidney injury

  • Ilaria Lippi
  • F. Perondi
  • V. Meucci
  • B. Bruno
  • V. Gazzano
  • G. Guidi
Original Article

Abstract

The aim of the present study was to evaluate the sensitivity and specificity of urine KIM-1 and urine GGT for the detection of naturally-occurring AKI, compared to healthy control dogs, dogs with stable chronic kidney disease (CKD), and dogs with lower urinary tract disorders (LUTD). The study included AKI grade 1 (n = 21), AKI grade 2 to 5 (n = 11), stable CKD (n = 11), LUTD (n = 15), and healthy dogs (n = 37). Urine KIM-1 (ng/mg) and GGT (U/l) were normalized to urine creatinine (uCr). Statistically significant difference in KIM/uCr (p = 0.0007) and GGT/uCr (p < 0.0001) was found among the study groups. Area under the curve (AUC) for KIM-1/uCr and GGT/uCr as predictors of AKI was 0.81 and 0.91 respectively. Values of KIM-1/uCr of 0.73 ng/mg and of GGT/uCr of 54.33 showed the best combination of sensitivity and specificity (75% and 75.6%; 85.7% and 89.1% respectively). A significant positive correlation (p < 0.0001) between KIM-1/uCr and GGT/uCr was found. Both urine KIM-1/uCr and GGT/uCr seemed to be potentially good markers for the diagnosis of AKI. Dogs with AKI showed significantly higher levels of urine KIM-1/uCr and urine GGT/uCr, compared with healthy dogs. Caution should be used in the evaluation of elevated urine KIM-1/uCr and GGT/uCr in dogs with pre-existing CKD and/or LUTD. Urine KIM-1/uCr and GGT/uCr might have a significant clinical utility, as complementary test, particularly in diagnosis early, non-azotemic stages of AKI.

Keywords

KIM-1 GGT AKI Dog 

Notes

Compliance with ethical standards

Conflict of interest

The Authors declare no conflict of interest. This paper was not supported by grants.

References

  1. Bruchim Y, Avital Y, Horoluitz M, Mazaki-Tovi M, Aroch I, Segev G (2017) Urinary heat shock protein 72 as a biomarker of acute kidney injury in dogs. Vet J 225:32–33CrossRefPubMedGoogle Scholar
  2. Clemo FA (1998) Urinary enzyme evaluation of nephrotoxicity in the dog. Toxicol Pathol 26:29–32CrossRefPubMedGoogle Scholar
  3. Cobrin AR, Blois SL, Kruth SA, Abrams-Ogg AC, Dewey C (2013) Biomarkers in the assessment of acute and chronic kidney diseases in the dog and the cat. J Small Anim Pract 54:647–655CrossRefPubMedGoogle Scholar
  4. Huang Y, Wauchope ACD (2011) The clinical utility of kidney injury molecule 1 in the prediction, diagnosis, and prognosis of acute kidney injury: a systematic review. Inflamm Allergy Drug Targets 10: 260–271CrossRefPubMedGoogle Scholar
  5. Ibba F, Mangiagalli G, Paltrinieri S (2016) Urinary gamma glutamyl transferase as a marker of tubular proteinuria in dogs with canine leishmaniasis, using sodium dodecylsulphate (SDS) electrophoresis as a reference method. Vet J 210:89–91CrossRefPubMedGoogle Scholar
  6. Jin Y, Shao X, Sun B, Miao C, Li Z, Shi Y (2017) Urinary kidney injury molecule as an early diagnostic biomarker of obstructive acute kidney injury and development of a rapid detection method. Mol Med Rep 15(3):1229–1235CrossRefPubMedPubMedCentralGoogle Scholar
  7. Lee YJ, Hu YY, Lin YS, Chang CT, Lin FY, Wong ML, Hsuan HK, Hsu WL (2012) Urine neutrophil gelatinase-associated lipogalin (NGAL) as a biomarker for acute canine kidney injury. BMC Vet Res 8:248CrossRefPubMedPubMedCentralGoogle Scholar
  8. Lianghos O, Tighiouart H, Perianayagam M, Koliada A, Han W, Wald R, Bonventre JV, Jaber BL (2009) Comparative analysis of urinary biomarkers for early detection of acute kidney injury following cardiopulmonary bypass. Biomarkers 14:423–443CrossRefGoogle Scholar
  9. Malyusz M, Braun D (1981) Enzymuria (the output of γ –glutamyl transpeptidase and of N-Acetyl β D glucosaminidase) in the course of experimental renovascular hypertension. Enzyme 26:32–42CrossRefPubMedGoogle Scholar
  10. Mancinelli E, Shaw DJ, Meredith AL (2012) Υ-glutamyl-transferase (GGT) activity in the urine of clinically healthy domestic rabbits (Oryctolagos Cuniculus). Vet Rec 171(19):475CrossRefPubMedGoogle Scholar
  11. Nivy R, Avital Y, Aroch I, Segev G (2017) Utility of urinary alkaline phosphatese and γ–glutamyl transpeptidase in diagnosing acute kidney injury in dogs. Vet J 220:43–47CrossRefPubMedGoogle Scholar
  12. Palm CA, Segev G, Cowgill LD, Le Roy BE, Kowalkowski KL, Kanakubo K, Westropp JL (2016) Urinary neutrophil gelatinase-associated lipocalin as a marker for identification of acute kidney injury and recovery in dogs with gentamicin-induced nephrotoxicity. J Vet Intern Med 30(1):200–205CrossRefPubMedPubMedCentralGoogle Scholar
  13. Rivers BJ, Walter PA, O’Brien TD, King VL, Polzin DJ (1996) Evaluation of urine gamma-glutamyl transpeptidase to creatinine ratio as a diagnostic tool in an experimental model of aminoglycoside-induced acute renal failure in the dog. J Am Anim Hosp Assoc 32(4):323–336CrossRefPubMedGoogle Scholar
  14. Waikar SS, Sabbisetti V, Arnlov J, Carlsson AC, Coresh J, Feldman HI, Foster MC, Fufaa GD, Helmersson-Karlqvist JH, Hsu CY, Kimmel PL, Larsson A, Liu Y, Lind L, Liu KD, Mifflin TE, Nelson RG, Riservs U, Vasan RS, Xie D, Zhang X, Bonventre JV (2016) Relationship of proximal tubular injury to chronic kidney disease as assessed by urinary kidney injury molecule-1 in five cohort studies. Nephrol Dial Transplant 31:1460–1470CrossRefPubMedPubMedCentralGoogle Scholar
  15. Yerramilli M, Farace G, Quinn J, Yerramilli M (2016) Kidney disease and the nexus of chronic kidney disease and acute kidney injury. The role of novel biomarkers and early and accurate diagnostics. Vet Clin North Am Small Anim Pract 46(6):961–963CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Dipartimento di Scienze VeterinarieUniversità di PisaPisaItaly
  2. 2.Dipartimento di Scienze VeterinarieUniversità di TorinoTorinoItaly

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