Abstract
Indigestible oligosaccharides have been shown to normalize blood glucose and insulin concentration thereby promoting good health and preventing diseases, such as diabetes. Transglucosidase (TG, α-glucosidase, enzyme code (EC) 3.2.1.20) is an enzyme capable of converting starch to oligosaccharides, such as iso-malto-oligosaccharides from maltose, via the action of amylase. The aim of this study was to evaluate whether oral administration of TG with maltose or dextrin is capable of reducing post-prandial serum glucose concentration in experimentally streptozotocin (STZ)-induced diabetic dogs fed on a high-fiber diet. Five healthy and five STZ-induced diabetic dogs were employed in this study. TG supplementation with dextrin or maltose had no detrimental effect in healthy dogs. In fact, TG and dextrin exhibited a flatlined serum glucose pattern, while reducing mean post-prandial serum insulin and glucose concentration as compared to control diet alone. When TG supplementation was tested in STZ-induced diabetic dogs under the context of a high fiber diet, a 13.8% and 23.9% reduction in mean glucose concentration for TG with maltose and dextrin, respectively was observed. Moreover, TG with dextrin resulted in a 13% lower mean post-prandial glucose concentration than TG with maltose, suggesting that dextrin may be a more efficient substrate than maltose when used at the same concentration (1 g/kg). Our results indicate that TG supplementation with diet can lead to lower postprandial glucose levels versus diet alone. However, the efficacy of TG supplementation may depend on the type of diet it is supplemented with. As such, TG administration may be useful for preventing the progression of diabetes mellitus and in its management in dogs.
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Abbreviations
- BW:
-
body weight
- DM:
-
diabetes mellitus
- IMO:
-
iso-malto- oligosaccharides
- RER:
-
resting energy requirement
- TG:
-
transglucosidase
References
Anderson, J.W. and Akanji, A.O., 1991. Dietary fiber - an overview. Diabetes Care, 14, 1126–1131
Arai, T., Nakamura, M., Magori, E., Fukuda, H., Mizutani, H., Kawakami, E. and Sako, T., 2002. Changes in activities of enzymes related to energy metabolism in peripheral leukocytes of diabetic dogs with glycemic control by intensive insulin treatment. Research in Veterinary Science, 73, 183-186
Boucher, J., Daviaud, D., Simeon-Remaud, M., Carpene, C., Saulnier-Blache, J.S., Monsan, P. and Valet, P., 2003. Effect of non-digestible gluco-oligosaccharides on glucose sensitivity in high fat diet fed mice. Journal of Physiology and Biochemistry, 59, 169–173
Chiba, S., 1997. Molecular mechanism in alpha-glucosidase and glucoamylase. Bioscience, Biotechnology, and Biochemistry, 61, 1233–1239
Chiba, S., Hiromi, K., Minamiura, N., Ohnishi, M., Shimomura, T., Suga, K., Suganuma, T., Tanaka, A., Tomioka, S. and Yamamoto, T., 1979. Quantitative study on anomeric forms of glucose produced by alpha-glucosidases. Journal of Biochemistry, 85, 1135–1141
Duan, K.J., Wang, S.S., Huang, S.F. and Sheu, D.C., 1993. Kinetic studies and mathematical model for enzymatic production of isomaltooligosaccharides from maltose. Report to National Science Council. R.O.C.
Duan, K.J., Sheu, D.C. and Lin, C.T., 1995. Transglucosylation of a fungal alpha-glucosidase. The enzyme properties and correlation of isomaltooligosaccharide production. Annals of the New York Academy of Sciences, 750, 325–328
Feldman, E.C. and Nelson, R.W., 2004. Diabetes mellitus. In: Canine and Feline Endocrinology and Reproduction. 3rd edn., (W.B.Saunders, Philadelphia) 486-538
Hesta, M., Debraekeleer, J., Janssens, G.P. and De Wilde, R., 2001. The effect of a commercial high-fiber diet and an iso-malto-oligosaccharide-supplemented diet on post-prandial glucose concentrations in dogs. Journal of Animal Physiology and Animal Nutrition, 85, 217–221
Hoenig. M., 2002. Comparative aspects of diabetes mellitus in dogs and cats. Molecular and Cellular Endocrinology, 197, 221-229
Kariya, K., Ogawa, T. and Joh, T., 2000. Effect on gut flora of oligosaccharide synthesizing enzymes. Digestion & Absorption, 23, 107–109
Kurimoto, M., Nishimoto, T., Nakada, T., Chaen, H., Fukuda, S. and Tsujisaka, Y., 1997. Synthesis by an alpha-glucosidase of glycosyl-trehaloses with an isomaltosyl residue. Bioscience, Biotechnology, and Biochemistry, 61, 699–703
Like, A.A., Appel, M.C., Williams, R.M., and Rossini, A.A., 1978. Streptozotocin-induced pancreatic insulitis in mice. Morphologic and physiologic studies. Laboratory Investigation, 38, 470-486
Mansford, K.R. and Opie, L., 1968. Comparison of metabolic abnormalities in diabetes mellitus induced by streptozotocin or by alloxan. Lancet, 291, 670–671.
Misawa, K., Ichikawa, K., Ojima, K., Hamano, S., Kitamura, T., and Komatsu, H., 2001. Effect of KAD-1229, a nonsulfonylurea hypoglycemic agent, on plasma glucose and insulin in streptozotocin-induced diabetic dogs. Pharmacology, 62, 65-72
Mori, A., Sako, T., Lee, P., Motoike, T., Iwase, K., Kanaya, Y., Fukuta, H., Mizutani, H., and Arai T. 2008. Comparison of time-action profiles of insulin glargine and NPH insulin in normal and diabetic dogs. Veterinary Research Communications, 32, 563-573
Nelson, R. 1992. Dietary management of diabetes mellitus. The Journal of Small Animal Practice, 33, 213-217
Nelson, R. and Sunvold, G., 1998. Effect of carboxymethylcellulose on postprandial glycaemic response in dogs. In: Recent advances in Canine and Feline Nutrition, Vol. 2; Reinheart, G. and Carrey, D. (Eds) Proc. IAMS Nutrition Symposium, Dayton , OH. Orange Frazer Press, Ohio. 97-102
Nuttal F.Q., 1993. Dietary fiber in the management of diabetes. Diabetes 42, 503-508
Pazur, J.H., Cepure, A., Okada, S. and Forsberg, L.S., 1977. Comparison of the action of glucoamylase and glucosyltransferase on D-glucose, maltose, and malto-oligosaccharides. Carbohydrate Research, 58, 193–202
Rand, J.S., Fleeman, L.M., Farrow ,H.A., Appleton, D.J., and Lederer R., 2004. Canine and feline diabetes mellitus: nature or nurture? The Journal of Nutrition, 134, 2072S-2080S
Sasaki, M., Joh, T., Koikeda, S., Kataoka, H., Tanida, S., Oshima, T., Ogasawara, N., Ohara, H., Nakao, H. and Kamiya, T., 2007. A novel strategy in production of oligosaccharides in digestive tract: prevention of postprandial hyperglycemia and hyperinsulinemia. Journal of Clinical Biochemistry and Nutrition, 41, 191-196
Sebbag, L., Forrat, R., Canet, E., Wiernsperger, N., Delaye, J., Renaud, S. and De Lorgeril, M., 1994. Effect of experimental non-insulin requiring diabetes on myocardial microcirculation during ischaemia in dogs. European Journal of Clinical Investigation, 24 686-690
Tobin, B.L. and Finegood, D.T., 1993. Reduced insulin secretion by repeated low doses of STZ impairs glucose effectiveness but does not induce insulin resistance in dogs. Diabetes, 42, 474-483
Acknowledgments
This work was supported in part by the 2008 Strategic Research Base Development Program for Private Universities from the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT).
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Sako, T., Mori, A., Lee, P. et al. Supplementing transglucosidase with a high-fiber diet for prevention of postprandial hyperglycemia in streptozotocin-induced diabetic dogs. Vet Res Commun 34, 161–172 (2010). https://doi.org/10.1007/s11259-010-9342-0
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DOI: https://doi.org/10.1007/s11259-010-9342-0