Plant Ecology

, Volume 218, Issue 4, pp 407–415 | Cite as

Intraspecific variability of ecophysiological traits of four Magnoliaceae species growing in two climatic regions in China

  • Q. Y. Xu
  • H. Liu
  • Q. Ye


Plants may change their ecophysiological traits to adapt to new environments, but the responses strongly depend on species and environmental conditions. Magnoliaceae species are of great scientific importance but are extremely endangered, therefore, it is crucial to study their ecophysiological adaptations for ex situ conservation. Here, we chose four common Magnoliaceae species growing in two botanical gardens located in south and north subtropical monsoon regions, and measured hydraulic and photosynthetic traits in both wet and dry seasons. We found that plants growing in north region showed significant lower leaf water potential at predawn and midday than those in south region, indicating that species suffered more severe drought stress in north region. As a result, species in north region had lower stomatal conductance and photosynthetic rates, as well as smaller stomatal pore index. In addition, significantly lower stem hydraulic conductivity of the two deciduous species in north region were observed compared with species in south region, while the two evergreen species at both regions showed similar values of stem hydraulic conductivity. Non-significant differences in leaf turgor loss points, leaf conductance, specific leaf area, and wood density were found when comparing species from the north and south regions. Our results suggested that the adjustment of plant hydraulics to local climatic conditions of Magnoliaceae species occurs primarily through changes in stomatal morphology and function, whereas the contribution of intraspecific variation in leaf hydraulic traits appears to be limited.


Climatic regions Hydraulic conductivity Leaf habit Local adaptation Phenotypic plasticity Stomatal regulation 



We are grateful to Mr. Yang Keming from the Horticulture Centre of South China Botanical Garden, and Drs. Quanfa Zhang, Qi Deng, and Longxing Hu from Wuhan Botanical Garden for their assistance in species identification and sampling. This work was funded by the National Natural Science Foundation of China (31670411).

Supplementary material

11258_2017_699_MOESM1_ESM.docx (12 kb)
Supplementary material 1 (DOCX 11 kb)


  1. Alder NN, Sperry JS, Pockman WT (1996) Root and stem xylem embolism, stomatal conductance, and leaf turgor in Acer grandidentatum populations along a soil moisture gradient. Oecologia 105:293–301. doi: 10.1007/BF00328731 CrossRefGoogle Scholar
  2. Aranda I et al. (2015) Variation in photosynthetic performance and hydraulic architecture across European beech (Fagus sylvatica L.) populations supports the case for local adaptation to water stress. Tree Physiol 35:34–46. doi: 10.1093/treephys/tpu101 CrossRefPubMedGoogle Scholar
  3. Arango-Velez A, Zwiazek JJ, Thomas BR, Tyree MT (2011) Stomatal factors and vulnerability of stem xylem to cavitation in poplars. Physiol Plant 143:154–165. doi: 10.1111/j.1399-3054.2011.01489.x CrossRefPubMedGoogle Scholar
  4. Bartlett MK, Scoffoni C, Sack L (2012) The determinants of leaf turgor loss point and prediction of drought tolerance of species and biomes: a global meta-analysis. Ecol Lett 15:393–405. doi: 10.1111/j.1461-0248.2012.01751.x CrossRefPubMedGoogle Scholar
  5. Brodribb TJ, Feild TS (2010) Leaf hydraulic evolution led a surge in leaf photosynthetic capacity during early angiosperm diversification. Ecol Lett 13:175–183. doi: 10.1111/j.1461-0248.2009.01410.x CrossRefPubMedGoogle Scholar
  6. Brodribb TJ, Holbrook NM, Edwards EJ, GutiÉRrez MV (2003) Relations between stomatal closure, leaf turgor and xylem vulnerability in eight tropical dry forest trees. Plant Cell Environ 26:443–450. doi: 10.1046/j.1365-3040.2003.00975.x CrossRefGoogle Scholar
  7. Bucci SJ, Scholz FG, Goldstein G, Meinzer FC, Hinojosa JA, Hoffmann WA, Franco AC (2004) Processes preventing nocturnal equilibration between leaf and soil water potential in tropical savanna woody species. Tree Physiol 24:1119–1127. doi: 10.1093/treephys/24.10.1119 CrossRefPubMedGoogle Scholar
  8. Bütof A, von Riedmatten LR, Dormann CF, Scherer-Lorenzen M, Welk E, Bruelheide H (2012) The responses of grassland plants to experimentally simulated climate change depend on land use and region. Glob Change Biol 18:127–137. doi: 10.1111/j.1365-2486.2011.02539.x CrossRefGoogle Scholar
  9. Choat B, Sack L, Holbrook NM (2007) Diversity of hydraulic traits in nine Cordia species growing in tropical forests with contrasting precipitation. New Phytol 175:686–698. doi: 10.1111/j.1469-8137.2007.02137.x CrossRefPubMedGoogle Scholar
  10. Cicuzza D, Newton A, Oldfield S, Botanic Gardens Conservation International (BGCI), Fauna & Flora International (FFI) (2007) The red list of Magnoliaceae. Fauna & Flora International, CambridgeGoogle Scholar
  11. Dandy JE (1927) The genera of Magnoliaceae. Kew Bull 7:257–264Google Scholar
  12. Davidson AM, Jennions M, Nicotra AB (2011) Do invasive species show higher phenotypic plasticity than native species and if so, is it adaptive? A meta-analysis. Ecol Lett 14:419–431. doi: 10.1111/j.1461-0248.2011.01596.x CrossRefPubMedGoogle Scholar
  13. Duputié A, Rutschmann A, Ronce O, Chuine I (2015) Phenological plasticity will not help all species adapt to climate change. Glob Change Biol. doi: 10.1111/gcb.12914 Google Scholar
  14. Franks PJ (2006) Higher rates of leaf gas exchange are associated with higher leaf hydrodynamic pressure gradients. Plant Cell Environ 29:584–592. doi: 10.1111/j.1365-3040.2005.01434.x CrossRefPubMedGoogle Scholar
  15. Hacke UG, Sperry JS (2001) Functional and ecological xylem anatomy. Perspect Plant Ecol 4:97–115. doi: 10.1078/1433-8319-00017 CrossRefGoogle Scholar
  16. Hao GY et al. (2008) Stem and leaf hydraulics of congeneric tree species from adjacent tropical savanna and forest ecosystems. Oecologia 155:405–415. doi: 10.1007/s00442-007-0918-5 CrossRefPubMedGoogle Scholar
  17. Holland N, Richardson AD, Goodale UM, Marshall P (2009) Stomatal length correlates with elevation of growth in four temperate species. J Sustain For 28:63–73. doi: 10.1080/10549810802626142 CrossRefGoogle Scholar
  18. IUCN (2001) IUCN Red List categories and criteria: version 3.1. IUCN Species Survival CommissionGoogle Scholar
  19. Jacobsen AL, Agenbag L, Esler KJ, Pratt RB, Ewers FW, Davis SD (2007a) Xylem density, biomechanics and anatomical traits correlate with water stress in 17 evergreen shrub species of the Mediterranean-type climate region of South Africa. J Ecol 95:171–183. doi: 10.1111/j.1365-2745.2006.01186.x CrossRefGoogle Scholar
  20. Jacobsen AL, Pratt RB, Davis SD, Ewers FW (2007b) Cavitation resistance and seasonal hydraulics differ among three arid Californian plant communities. Plant Cell Environ 30:1599–1609. doi: 10.1111/j.1365-3040.2007.01729.x CrossRefPubMedGoogle Scholar
  21. Kim S, Suh Y (2013) Phylogeny of Magnoliaceae based on ten chloroplast DNA regions. J Plant Biol 56:290–305. doi: 10.1007/s12374-013-0111-9 CrossRefGoogle Scholar
  22. Kreyling J, Thiel D, Simmnacher K, Willner E, Jentsch A, Beierkuhnlein C (2012) Geographic origin and past climatic experience influence the response to late spring frost in four common grass species in central Europe. Ecography 35:268–275. doi: 10.1111/j.1600-0587.2011.07173.x CrossRefGoogle Scholar
  23. Kröber W, Heklau H, Bruelheide H (2015) Leaf morphology of 40 evergreen and deciduous broadleaved subtropical tree species and relationships to functional ecophysiological traits. Plant Biol 17:373–383. doi: 10.1111/plb.12250 CrossRefPubMedGoogle Scholar
  24. Ladjal M, Huc R, Ducrey M (2005) Drought effects on hydraulic conductivity and xylem vulnerability to embolism in diverse species and provenances of Mediterranean cedars. Tree Physiol 25:1109–1117. doi: 10.1093/treephys/25.9.1109 CrossRefPubMedGoogle Scholar
  25. Lens F, Sperry JS, Christman MA, Choat B, Rabaey D, Jansen S (2011) Testing hypotheses that link wood anatomy to cavitation resistance and hydraulic conductivity in the genus Acer. New Phytol 190:709–723. doi: 10.1111/j.1469-8137.2010.03518.x CrossRefPubMedGoogle Scholar
  26. Liu H, Xu Q, He P, Santiago LS, Yang K, Ye Q (2015) Strong phylogenetic signals and phylogenetic niche conservatism in ecophysiological traits across divergent lineages of Magnoliaceae. Sci Rep 5:12246. doi: 10.1038/srep12246 CrossRefPubMedPubMedCentralGoogle Scholar
  27. Macel M et al. (2007) Climate vs. soil factors in local adaptation of two common plant species. Ecology 88:424–433. doi: 10.1890/0012-9658(2007)88[424:CVSFIL]2.0.CO;2 CrossRefPubMedGoogle Scholar
  28. Nardini A, Gortan E, Salleo S (2005) Hydraulic efficiency of the leaf venation system in sun- and shade-adapted species. Funct Plant Biol 32:953–961. doi: 10.1071/FP05100 CrossRefGoogle Scholar
  29. Nicotra AB et al. (2010) Plant phenotypic plasticity in a changing climate. Trends Plant Sci 15:684–692. doi: 10.1016/j.tplants.2010.09.008 CrossRefPubMedGoogle Scholar
  30. Nobel P (1991) Physiochemical and environmental plant physiology. Academic Press, pp 99–170Google Scholar
  31. R Development Core Team (2013) R: a language and environment for statistical computing. R Foundation for Statistical Computing, ViennaGoogle Scholar
  32. Robson TM, Sánchez-Gómez D, Cano FJ, Aranda I (2012) Variation in functional leaf traits among beech provenances during a Spanish summer reflects the differences in their origin. Tree Genet Genome 8:1111–1121. doi: 10.1007/s11295-012-0496-5 CrossRefGoogle Scholar
  33. Robson TM, Rasztovits E, Aphalo PJ, Alia R, Aranda I (2013) Flushing phenology and fitness of European beech (Fagus sylvatica L.) provenances from a trial in La Rioja, Spain, segregate according to their climate of origin. Agr For Meteorol 180:76–85. doi: 10.1016/j.agrformet.2013.05.008 CrossRefGoogle Scholar
  34. Sack L, Frole K (2006) Leaf structural diversity is related to hydraulic capacity in tropical rain forest trees. Ecology 87:483–491. doi: 10.1890/05-0710 CrossRefPubMedGoogle Scholar
  35. Sack L, Holbrook NM (2006) Leaf hydraulics. Annu Rev Plant Biol 57:361–381. doi: 10.1146/annurev.arplant.56.032604.144141 CrossRefPubMedGoogle Scholar
  36. Sack L, Cowan P, Jaikumar N, Holbrook N (2003) The ‘hydrology’ of leaves: co-ordination of structure and function in temperate woody species. Plant Cell Environ 26:1343–1356. doi: 10.1046/j.0016-8025.2003.01058.x CrossRefGoogle Scholar
  37. Sánchez-Gómez D, Robson TM, Gascó A, Gil-Pelegrín E, Aranda I (2013) Differences in the leaf functional traits of six beech (Fagus sylvatica L.) populations are reflected in their response to water limitation. Environ Exp Bot 87:110–119. doi: 10.1016/j.envexpbot.2012.09.011 CrossRefGoogle Scholar
  38. Santiago LS, Goldstein G, Meinzer FC, Fisher JB, Machado K, Woodruff D, Jones T (2004) Leaf photosynthetic traits scale with hydraulic conductivity and wood density in Panamanian forest canopy trees. Oecologia 140:543–550. doi: 10.1007/s00442-004-1624-1 CrossRefPubMedGoogle Scholar
  39. Schulte P, Hinckley T (1985) A comparison of pressure-volume curve data analysis techniques. J Exp Bot 36:1590–1602. doi: 10.1093/jxb/36.10.1590 CrossRefGoogle Scholar
  40. Sperry JS, Donnelly JR, Tyree MT (1988) A method for measuring hydraulic conductivity and embolism in xylem. Plant Cell Environ 11:35–40. doi: 10.1111/j.1365-3040.1988.tb01774.x CrossRefGoogle Scholar
  41. Sultan SE (2000) Phenotypic plasticity for plant development, function and life history. Trends Plant Sci 5:537–542. doi: 10.1016/S1360-1385(00)01797-0 CrossRefPubMedGoogle Scholar
  42. Tyree MT, Hammel HT (1972) The measurement of the turgor pressure and the water relations of plants by the pressure-bomb technique. J Exp Bot 23:267–282. doi: 10.1093/jxb/23.1.267 CrossRefGoogle Scholar
  43. Villar-Salvador P, Castro-Díez P, Pérez-Rontomé C, Montserrat-Martí G (1997) Stem xylem features in three Quercus (Fagaceae) species along a climatic gradient in NE Spain. Trees 12:90–96. doi: 10.1007/PL00009701 Google Scholar
  44. Willson CJ, Manos PS, Jackson RB (2008) Hydraulic traits are influenced by phylogenetic history in the drought-resistant, invasive genus Juniperus (Cupressaceae). Am J Bot 95:299–314. doi: 10.3732/ajb.95.3.299 CrossRefPubMedGoogle Scholar
  45. Zhang YJ, Holbrook NM, Cao KF (2014) Seasonal dynamics in photosynthesis of woody plants at the northern limit of Asian tropics: potential role of fog in maintaining tropical rainforests and agriculture in Southwest China. Tree Physiol. doi: 10.1093/treephys/tpu083 Google Scholar
  46. Zhu SD, Song JJ, Li RH, Ye Q (2013) Plant hydraulics and photosynthesis of 34 woody species from different successional stages of subtropical forests. Plant Cell Environ 36:879–891. doi: 10.1111/pce.12024 CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  1. 1.Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems, South China Botanical GardenChinese Academy of SciencesGuangzhouChina
  2. 2.University of Chinese Academy of SciencesBeijingChina
  3. 3.Guangdong Provincial Key Laboratory of Applied BotanySouth China Botanical Garden, Chinese Academy of SciencesGuangzhouChina

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