Seroepidemiological study of Caseous lymphadenitis in sheep from the Northeast region of Brazil using an indirect ELISA

Abstract

The objective of this study was to determine the frequency of seropositivity Corynebacterium pseudotuberculosis in sheep in five states of northeastern Brazil, using an indirect enzyme-linked immunosorbent assay (i-ELISA). Young and adult sheep of both sexes were used. Blood samples were collected from 2638 sheep from 223 herds across all states. For the i-ELISA, antigens produced from the strain of C. pseudotuberculosis BRM 029971, a bacterial isolate from the Northeast region of Brazil, were used. Sensitivity and specificity indexes were calculated for the validation of the test, using as reference 49 and 134 serum samples from sheep known to be positive and negative, respectively. The i-ELISA presented four false-negative and four false-positive results, showing a specificity of 97.01%, a sensitivity of 91.84%, and an accuracy of 95.63%. These results were calculated based on an optical density (OD) cutoff point = 0.138. Of the 2638 sheep tested, 996 (37.76%, 95% CI = 35.93–39.62%) were seropositive, and of the 223 evaluated herds, 210 (94.17%, 95% CI = 90.28–96.56%) seropositive. The i-ELISA showed adequate sensitivity and specificity, proving to be a useful tool in the diagnosis of caseous lymphadenitis (CLA) in sheep. Infection by C. pseudotuberculosis, determined by serology, is disseminated in the sheep herds in the states of Northeast Brazil. Thus, there is a need to implement effective control measures that prevent the spread of infectious agents.

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Acknowledgments

The authors thank the Departamento de Sanidade Animal, Embrapa Caprinos e Ovinos, Sobral, CE, for the research opportunity and cooperation. Thanks also to Professor Dr. Ricardo Wagner Dias Portela from the Universidade Federal da Bahia for notes regarding the indirect ELISA test.

Funding

This research is financially supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico/ Ministério da Agricultura Pecuária e Abastecimento through Public Announcement 64/2008.

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Correspondence to José Romero Alexandre Alves.

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This project was submitted to the Comitê de Ética em Pesquisa from Centro de Saúde e Tecnologia Rural da Universidade Federal de Campina Grande and approved according to protocol number 012/2017.

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Alves, J.R.A., de Farias, A.E.M., dos Anjos, D.M. et al. Seroepidemiological study of Caseous lymphadenitis in sheep from the Northeast region of Brazil using an indirect ELISA. Trop Anim Health Prod 52, 1945–1952 (2020). https://doi.org/10.1007/s11250-020-02214-9

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Keywords

  • Brazilian northeast
  • Caseous lymphadenitis
  • Enzyme-linked immunosorbent assay
  • Sheep
  • Serology