Evaluation of a cattle rapid test for early pregnancy diagnosis in sheep

Abstract

The early pregnancy diagnosis allows optimizing production and timely management correction, with a greater reproductive output of livestock. The Idexx Rapid Visual Pregnancy Test® consists of an ELISA for visual reading which does not require the use of readers in the laboratory, with satisfactory pregnancy diagnoses at 28 days of pregnancy in cattle. Therefore, the aim of this study was to evaluate this rapid test and to verify the most appropriate day for the diagnosis of pregnancy in the ovine species. For this purpose, 98 serum samples from pregnant sheep and 36 from non-pregnant were used, with duplicate samples, and diagnosis was confirmed by ultrasound examination, used as the gold standard. The numbers of positive samples obtained at 26, 28, and 30 days of pregnancy were 26, 27, and 45, respectively. The Rapid Visual Pregnancy Test correctly identified 100% of the samples as positive at pregnancy of days 26, 28, and 30. The sensitivity and specificity of the test were also 100%. The Idexx Rapid Visual Pregnancy Test, initially indicated for cattle, is effective for the detection of pregnancy in the ovine species, enabling diagnosis of pregnant sheep from the 26th day of pregnancy.

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Acknowledgments

Camila de Miranda e Silva Chaves received a scholarship financed by CAPES, which is gratefully acknowledged.

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Correspondence to Camila de Miranda e Silva Chaves.

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This study was approved by the Instituto de Zootecnia’s Ethics Committee (Protocol CEUA/IZ No.200/2014).

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de Miranda e Silva Chaves, C., da Costa, R.L.D., Duarte, K.M.R. et al. Evaluation of a cattle rapid test for early pregnancy diagnosis in sheep. Trop Anim Health Prod 52, 1345–1349 (2020). https://doi.org/10.1007/s11250-019-02130-7

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Keywords

  • Pregnancy-associated glycoprotein
  • Elisa
  • Visual test
  • Gestation diagnosis