The aim was to evaluate the effect of bee pollen (BP) on performance, blood parameters, and metabolic hormones in New Zealand White (NZW) and Rex (RX) rabbits. A total of 60 six-week-old rabbits were allotted into a completely randomized design in a 2 × 3 factorial arrangement (two breeds and three levels of BP: 0, 250, and 350 mg/kg body weight). The effects of breed × BP supplementation level were non-significant for all growth performance and carcass traits, as well as the concentrations of metabolic hormones in the plasma. Meanwhile, some blood parameters (hemoglobin, packed cell volume, alanine aminotransferase, aspartate aminotransferase, and urea) were significantly affected (P < 0.05). Regardless of the effect of breed, the supplementation of BP at a dose of 350 mg/kg BW increased the final body weight (P = 0.008), average daily gain (P < 0.001), and net profits (P = 0.006). However, the rabbits treated with BP at a dose of 250 mg/kg BW showed lower average daily feed intake (− 14.45 g/d; P = 0.049) and feed to gain ratio (− 0.48; P = 0.039). In both rabbit breeds, BP at a dose of 350 mg/kg increased the concentrations of insulin-like growth factor-1 (302.62 ng/ml, P < 0.001), insulin (16.63 ng/ml, P = 0.050), and triiodothyronine (158.50 ng/dl, P < 0.001) in plasma compared with other groups. It may be concluded that intake of BP supplementation can improve the feed to gain ratios, blood indices, and metabolic hormones in different rabbit breeds. However, the appropriate dosage and the route of supplementation of BP in broiler rabbits need further research.
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Abdel-Azeem, A.S., Abdel-Azim, A.M., Darwish, A.A., Omar, E.M., 2010. Haematological and biochemical observations in four pure breeds of rabbits and their crosses under Egyptian environmental conditions. World Rabbit Sci, 18, 103–110.
Abdel-Hamid, T.M., Farahat, M.H., 2016. Effect of dietary mannan-oligosaccharides on some blood biochemical, haematological parameters and carcass traits in purebred New Zealand White and crossbred rabbits. Anim. Prod. Sci, 56, 2133–2139.
Abreu, M., 1992. El polen como alimento en la nutricion humana. In alimentaria., 235, 45–46.
Agarwal, S.K., Cogburn, L.A., Burnside, J., 1994. Dysfunctional growth hormone receptor in a strain of sex-linked dwarf chickens: evidence for a mutation in the intracellular domain. Journal of Endocrinology, 142, 427-434.
Al-Dobaib, S.N., Khalil, M.H., Hashad, M., Al-Saef, A.M., 2007. Growth, carcass and caecal traits in V-line and crossbred rabbits fed diets containing discarded dates. World Rabbit Sci, 15, 81-90.
Anitha, K., 2007. Genetic studies on the performance of certain broiler rabbit breeds. Master of Veterinary Science, Faculty of Veterinary Science, Animal Genetics and Breeding Department, Sri Venkateswara Veterinary University, Rajendranagar, Hyderabad-500 030.
AOAC. 1990. Association of Official Agricultural Chemists. Official methods of analysis. Washington DC.
Attia, Y.A., Abd El Hamid, A.E., Bovera, F., El-Sayed, M.I., 2009. Reproductive and productive performance of rabbit does submitted to an oral glucose supplementation. Animal, 3, 1401–1407.
Attia, Y.A., Al-Hanoun, A., El-Din, A.E., Bovera, F., Shewika, Y.E., 2011a. Effect of bee pollen levels on productive, reproductive and blood traits of NZW rabbits. J. Anim. Physiol. Anim. Nutr, 95, 294-303.
Attia, Y.A., Al-Hanoun, A., Bovera, F., 2011b. Effect of different levels of bee pollen on performance and blood profile of New Zealand White bucks and growth performance of their offspring during summer and wither months. J. Anim. Physiol. Anim. Nutr, 95, 17-26.
Attia, Y.A., El-Hanoun, A.M., Bovera, F., Monastra, G., El-Tahawy, W.S., Habiba, H.I., 2014. Growth performance, carcass quality, biochemical and haematological traits and immune response of growing rabbits as affected by different growth promoters. J. Anim. Physiol. Anim. Nutr, 98, 128-139.
Attia, Y., Bovera, F., El-Tahawy, W., El-Hanoun, A., Al-Harthi, M., Habiba, H., 2015. Productive and reproductive performance of rabbits does as affected by bee pollen and/or propolis, inulin and/or mannan-oligosaccharides. World Rabbit Sci, 23, 273–282.
Bell, R.R., Thornber, E.J., Seet, J.L., Groves, M.T., Ho, N.P., Bell, D.T., 1983. Composition and protein quality of honeybee-collected pollen of Eucalyptus marginata and Eucalyptus calophylla. The Journal of nutrition, 113, 2479-2484.
Blasco, A., Ouhayoun, J., 1996. Harmonization of criteria and terminology in rabbit meat research: revised proposal. World Rabbit Sci, 4, 93–99.
Broadhurst, C.L., 1999. Bee products: medicine from the hive. Issue of Nutrition Science News, August.
Burnside, J., Liou, S.S., Cogburn, L.A., 1991. Molecular cloning of the chicken growth hormone receptor complementary deoxyribonucleic acid: mutation of the gene in sex-linked dwarf chickens. Endocrinology, 128, 3183-3192.
Cline, M.J., Hutton, J.J., 1983. Hematology and oncology. In: Stein J.H. (ed). Internal Medicine, Boston, Little, Brown.
Coles, E.H., 1986. Veterinary Clinical Pathology. WB Saunders, Philadelphia, 4th ed.
Crane E. 1990. Bees and Beekeeping, Science. Practice and World Resources. Cornstock Publ., Ithaca., 593–594.
Dias, D.M.B., Oliveira, M.C., Silva, D.M., Bonifácio, N.P., Claro, D.C., Marchesin, W.A. 2013. Bee pollen supplementation in diets for rabbit does and growing rabbits. Acta Sci. Anim. Sci, 35, 425-430.
El-Neney, B.A.M., El-Kholy, K.H., 2014. Effect of natural additive (bee pollen) on immunity and productive and reproductive performances in rabbits. 1- Growth performance, digestive and immune responses in growing rabbits. Egypt. Poult. Sci, 34, 579-606.
Elsaesser, F., Pfaffl, M.W., Meyer, H.H.D., Serpek, B., Sauerwein, H., 2002. Differences in the somatotropic axis, in blood cortisol, insulin and thyroid hormone concentrations between two pig genotypes with markedly divergent growth rates and the effects of growth hormone treatment. Animal Science, 74, 423-430.
El-Sheikh, T.M., Mona Ghaly, M., Selem, T.S.T., 2011. Comparative studies on some productive capabilities among imported, endogenous and native rabbit breeds under Egyptian environmental conditions. Seria Zootehnie, 56, 364–369.
Fathi, M., Abdelsalam, M., Al-Homidan, I., Ebeid, T., El-Zarei, M., Abou-Emera, O., 2017. Effect of probiotic supplementation and genotype on growth performance, carcass traits, hematological parameters and immunity of growing rabbits under hot environmental conditions. Animal Science Journal, 88, 1644-1650.
Fazayeli-Rad, A.R., Afzali, N., Farhangfar, H., Asghari, M.R., 2015. Effect of bee pollen on growth performance, intestinal morphometry and immune status of broiler chicks. European Poultry Science, 79, pp 1-9.
Jerry Kaneko, J., 2008. Clinical Biochemistry of Domestic Animals (Sixth Edition), Chapter 20-Thyroid Function, 623-634.
Leja, M., Mareczek, A., Wyzgolik, G., Klepacz-Baniak, J., Czekon´ska, K., 2007. Antioxidative properties of beepollen in selected plant species. Food Chem, 100, 237–240.
Lucky, Z., 1977. Methods for the diagnosis of fish diseases, Ameruno Publishing Co, PVT, LTd. New York.
Maj, D., Bieniek, J., Łapa, P., Ina, S., 2009. The effect of crossing New Zealand White with Californian rabbits on growth and slaughter traits. Arch. Tierz, 52, 205–211.
Markham, K.R., Campos, M., 1996. 7- and 8-0-methyl-herbacetin-3-0-sophorosides from bee pollens and some structure/activity observations. Phytochemistry, 43, 763–767.
McNitt J.I., Lukefahr S.D., Cheeke P.R., Patton N.M. 2013. Rabbit production. British Library, London, UK, Chaper1 (Introduction), Ninth ed., pp 4.
Nasr, M.A.F., Abd-Elhamid, T., Hussein, M.A., 2017. Growth performance, carcass characteristics, meat quality and muscle amino-acid profile of different rabbits breeds and their crosses. Meat Sci, 134, 150–157.
Ng’ambi, J.W., Maoba, S.M., Norris, D., Malatje, M.S., Mbajiorgu, C.A., 2009. Effect of dietary lysine to crude protein ratio on performance of male Ross 308 broiler chickens. Tropical Animal Health and Production, 41, 11-16.
Ortiz-Hernandez, J.A., Rubio-Lozano, M.S., 2001. Effect of breed and sex on rabbit carcass yield and meat quality. World Rabbit Sci, 9, 51–56.
Ouyed, A., Rives, J., Brun, J.M., 2011. Heterosis, direct and maternal additive effects on rabbit growth and carcass traits from a Canadian experiment. World Rabbit Sci, 19, 31–41.
Quian Wei, L., Khan, Z., Watson, D.G., Fearnley, J., 2008. Analysis of sugars in bee pollen and propolis by ligand exchange chromatography in combination with pulsed amperometric detection and mass spectrometry. J. Food Compost. Anal, 21, 78–83.
Rinderknecht, E., Humbel, R.E., 1978. The amino acid sequence of human insulin-like growth factor I and its structural homology with proinsulin. J Biol Chem, 253, 2769–76.
Rosebrough, R.W., McMurtry, J.P., 1993. Protein and energy relationships in the broiler chicken. Effects of protein quantity and quality on metabolism. British Journal of Nutrition, 70, 667–678.
Salah, A.S., El-Tarabany, M.S., Ali, M.A., 2018. Impact of dietary supplementation with a synbiotic, organic acids or their combination on growth performance, carcass traits, economic efficiency, jejunum histomorphometry and some blood indices of broiler chickens. Animal Production Science. https://doi.org/10.1071/AN18156.
Šarić, A., Balog, T., Soboc´anec, S., Kusˇic, B., Sˇverko, V., Rusak, G., Likic, S., Bubalo, D., Pinto, B., Reali, D., Marotti, T., 2009. Antioxidant effects of flavonoid from Croatian Cystus incanus L. rich bee pollen. Food Chem. Toxicol, 47, 547–554.
Scanes, C.G., 2011. Hormones and Metabolism in Poultry, Update on Mechanisms of Hormone Actio-Focus on Metabolism, Growth and Reproduction, Prof. Gianluca Aimaretti (ed), ISBN: 978-953-307-341-5, InTech, Available from: http://www.intechopen.com/books/update-on-mechanisms-of-hormone-action-focuson-metabolism-growth-and-reproduction/hormones-and-metabolism-in-poultry
Scanes, C.G., Marsh, J., Decuypere, E., Rudas, P., 1983. Abnormalities in the plasma concentration of thyroxine, triiodothyronine and growth hormone in sex-linked dwarf and autosomal dwarf white leghorn domestic fowl (Gallus domesticus). Journal of Endocrinology, 97, 127-135.
Scanes, C.G., Proudman, J.A., Radecki, S.V., 1999. Influence of Continuous Growth Hormone or Insulin-Like Growth Factor I Administration in Adult Female Chickens. General and Comparative Endocrinology, 114, 315-323.
Singh, K., 1983. Effect of heat stress on blood constituents in crossbred heifers. Indian J. Anim. Sci, 53, 353–355.
Song, Y.F., Wang, J., Li, S.H., Shang, C.F., 2005. Effect of bee pollen on the development of digestive gland of broilers. Anim. Husb. Vet. Med. 37, 14-17.
Stick, D.A., Davis, M.E., Loerch, S.C., Simmen, R.C.M., 1998. Relationship between blood serum insulin-like growth factor I concentration and postweaning feed efficiency of crossbred cattle at three levels of dietary intake. J. Anim. Sci. 76, 498-505.
Stryer, L., 1995. Biochemistry (Fourth ed.). New York: W.H. Freeman and Company. 773-74.
Voet, D., Voet, J.G., 2011. Biochemistry (4th ed.). New York: Wiley.
Wang, J; Y, Su; M. A. Elzo; X, Jia; S, Chenand, S, Lai 2016. Comparison of carcass and meat quality traits among three rabbit breeds. Korean J. Food Sci. An. 36, 84-89.
Wintrobe, P.M., 1965. Clinical hematology. Lead and Febiger. Philadelphia.
Xu, X., Sun, L., Dong, J., Zhang, H., 2009. Breaking the cells of rape bee pollen and consecutive extraction of functional oil with supercritical carbon oxide. Innov. Food Sci. Emerg. Technol, 10, 42–46.
Zhang, Y.C., Li, F.C., 2010. Effect of dietary methionine on growth performance and insulin-like growth factor-I mRNA expression of growing meat rabbits. Journal of Animal Physiology and Animal Nutrition, 94, 803-809.
The experimental procedures of the current research were in accordance with the guidelines of the Committee of Animal Wealth Development Department, Zagzaig University, Egypt (ANWD 206).
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Abdel-Hamid, T.M., El-Tarabany, M.S. Effect of bee pollen on growth performance, carcass traits, blood parameters, and the levels of metabolic hormones in New Zealand White and Rex rabbits. Trop Anim Health Prod 51, 2421–2429 (2019). https://doi.org/10.1007/s11250-019-01961-8
- Bee pollen
- Blood parameters
- Growth performance