Abstract
The variability in breeding program leads to rapid loss of genetic potential for which National Bureau of Animal Genetic Resources is emphasized to conserve the indigenous breeds. The variation in myostatin (MSTN) gene and its association with growth traits will throw light on its potential use as marker in selection. Hence, the study was conducted to detect polymorphism in exon 3 of MSTN, one of the most important growth regulatory gene and its association with growth in Nilagiri sheep breed. Blood samples were collected from Nilagiri sheep (n = 103) of South India and growth data up to 1 year of age was recorded. Genomic DNA was isolated and amplified for part of MSTN gene; PCR products were genotyped by restriction digestion (MspI) and confirmed by sequencing. Restriction digestion has revealed a single nucleotide polymorphism at locus G5622C in exon 3 which was confirmed by sequencing. The wild-type DNA molecule (MM) cleaved by MspI produced 301-bp and 314-bp fragments and those with mutation (mm) would remain undigested. The genotypic frequencies were MM (0.689) and Mm (0.311) with complete absence of mm genotype; and allelic frequencies were M (0.8445) and m (0.1555). The locus was in Hardy-Weinberg equilibrium. The association analysis revealed that there was no significant difference in mean birth, weaning, 6-, 9-, and 12-month weight between MM and Mm genotypes at g.5622G>C locus of exon 3 of MSTN gene. This is the first report of mutation in exon 3 of MSTN gene. The non-significant effect and absence of mm genotype at this locus needs further studies based on large population size and haplotype analysis.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
19th Livestock Census, 2012. Department of Animal Husbandry and Dairying, Ministry of Agriculture, Government of India, New Delhi.
Archibald, A.L., Cockett, N.E., Dalrymple, B.P., Faraut, T., Kijas, J.W., Maddox, J.F., McEwan, J.C., Hutton, Oddy V., Raadsma, H.W., Wade, C., Wang, J., Wang, W., Xun, X., 2010. The sheep genome reference sequence: a work in progress. Animal Genetics, 41, 449–453.
Bellinge, R.H.S., Liberles, D.A., Iaschi, S.P.A., Obrien, A., Tay, G.K., 2005. Myostatin and its implications on animal breeding: a review. Animal Genetics, 36, 1–6.
Bhatia, S., Arora, R. 2005. Biodiversity and conservation of Indian sheep genetic resources – an overview. Asian-Australasian Journal of Animal Sciences, 18, 1387–1402.
Broad, T.E., Glass, B.C., Greer, G.J., Robertson, T.M., Bain, W.E., Lord, E.A., McEwan, J.C., 2000. Search for a locus near to myostatin that increases muscling in Texel sheep in New Zealand. Proceedings of New Zealand Society of Animal Production, 60, 110–112.
Dehnavi, E., Azari, M.A., Hasani, S., Nassiry, M.R., Mohajer, M., Ahmadi, A.K., Shahmohamadi, L., Yousefi, S., 2012. Polymorphism of myostatin gene in Intron 1 and 2 and Exon 3, and their associations with yearling weight, using PCR-RFLP and PCR-SSCP techniques in Zel sheep. Biotechnology Research International. Article ID 472307, 5 pages. https://doi.org/10.1155/2012/472307.
Falconer, D.S., Mackay, T.F.C., 1996. Introduction to Quantitative Genetics (4th Ed.). Longman Group Limited, England.
FAOSTAT, 2014. http://www.faostat3.fao.org.
Gan, S.Q., Du, Z., Liu, S.R., Yang, Y.L., Shen, M., Wang, X.H., Yin, J.L., Hu, X.X., Fei, J., Fan, J.J., Wang, J.H, He, Q.H., Zhang, Y.S., Li, N., 2008. Association of SNP haplotypes at the myostatin gene with muscular hypertrophy in sheep. Asian-Australasian Journal of Animal Sciences, 21, 928–935.
Ganesakale, D., Rathnasabapathy, V., 1973. Sheep breeds of Tamil Nadu. Cheiron, 2, 146–155.
Girish, H., Sivaselvam, S.N., Karthickeyan, S.M.K. Saravanan, R., 2007. Molecular Characterisation of Nilagiri Sheep (Ovis aries) of South India Based on Microsatellites. Asian-Australasian Journal of Animal Sciences, 20 (5), 633–637.
Grobet, L., Pncelet, D., Royo, L.J., Brouwers, B., Pirottin, D., Michau, C., Menissier, F., Zanotti, M., Dunner, S., George, M., 1998. Molecular definition of an allelic series of mutations disrupting the myostatin function and causing double-muscling. Mammalian Genome, 9, 210–213.
Hadjipavlou, G., Matika, O., Clop, A., Bishop, S.C., 2008. Two single nucleotide polymorphisms in the myostatin (GDF8) gene have significant association with muscle depth of commercial Charollais sheep. International Society for Animal Genetics. Animal Genetics, 39, 346–353.
Harvey, W.R., 1990. Mixed Model Least-squares and Maximum Likelihood Computer Programme.PC-2 version. Ohio State University, Colombus.
Kambadur, R., Sharma, M., Smith, T.P.L., Bass, J.J., 1997. Mutations in myostatin (GDF8) in double-muscled Belgian Blue and Piedmontese cattle. Genome Research, 7, 910–916.
Li, XL, Wu, ZL, Gong, YF, Liu, YQ, Liu, ZZ, Wang, XJ, Xin, TR, Ji, Q., 2006. Single nucleotide polymorphism identification in the caprine myostatin gene. Journal of Animal Breeding and Genetics, 123, 141–144.
Littlewood, R.W., 1936. Livestock of Southern India. Government of Madras, Madras, pp. 202–216.
Masoudi, A., Hemrani, H., Abbasi, A., 2005. Using PCR-SSCP techniques to study polymorphism of myostatin gene and its association with production traits in Baluchi sheep. Proceedings of 4th National Biotechnology Congress, Iran, Kerman, Iran.
McPherron, A.C., Lee, S.J., 1997. Double muscling in cattle due to mutations in the myostatin gene. Proceedings of the National Academy of Sciences, USA, 94, 12457–12461.
Rao, K.M.V., Schinckel, P.G., Clarke, W.H., 1960. The wool follicle population of some Indian breeds of sheep. Australian Journal of Agricultural Research, 11, 99–104.
Report, 2008. Sheep Breeding Research Station, Sandynallah. TANUVAS, Chennai, (available at http://www.tanuvas.ac.in/SBRS.html).
Sahu, A.R., Jeichitra, V., Rajendran, R., Raja, A., 2017. Polymorphism in exon 3 of myostatin (MSTN) gene and its association with growth traits in Indian sheep breeds. Small Ruminant Research, 149, 81–84.
Sambrook, J.E., Fritsch, F., Maniatis, T., 1989. Molecular Cloning: A Laboratory Manual, 2nd Ed. Cold spring Harbor Laboratory Press, New York, USA.
Singh, V.K., Mishra, A.K., Kumar, S., 2005. Genetic improvement of sheep for wool and mutton production in India: A Review. Indian Journal of Animal Sciences, 75 (3), 356–364.
Sudhakar, A. Rajendran, A., Rahumathulla, P.S., 2013. Detection of Booroola (FecB) mutation in Indian sheep – Nilagiri. Small Ruminant Research, 113, 55–57.
Task Force Report, 1996. Report on the Task Force on sheep, goat and rabbit production, Ministry of Agriculture, Department of Animal Husbandry & Dairying, Govt. of India.
Venkataramanan, R., Subramanian, A., Sivaselvam, S.N., Sivakumar, T., Sreekumar, C., Anilkumar, R., Iyue, M., 2013. Pedigree analysis of the Nilagiri sheep of South India. Animal Genetic Resources, 53, 11–18.
Zhou, H., Hickford, J.G.H., Fang, Q., 2008. Variation in the coding region of the myostatin (GDF8) gene in sheep. Molecular and Cellular Probes, 22, 67–68.
Acknowledgments
The authors are thankful to the Sheep Breeding Research Station, Sandynallah, TANUVAS, for the necessary support to carry out the research work.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Additional information
Publisher’s note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Sahu, A.R., V., J., R., R. et al. Novel report on mutation in exon 3 of myostatin (MSTN) gene in Nilagiri sheep: an endangered breed of South India. Trop Anim Health Prod 51, 1817–1822 (2019). https://doi.org/10.1007/s11250-019-01873-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11250-019-01873-7