Investigating peste des petits ruminants (PPR) in naturally infected goats and sheep in Anseba Region, Eritrea, by reverse transcription polymerase chain reaction (RT-PCR)
The impact of peste des petits ruminants (PPR) virus was investigated by reverse transcription polymerase chain reaction (RT-PCR) on different samples obtained from non-vaccinated diseased and necropsied sheep and goats showing PPR-like symptoms. The disease picture was typical and sheep were observed to be less susceptible. Nasal and rectal swabs, whole blood and pathological tissue samples from the lungs, intestine, and mesenteric lymph nodes were used for this study. The results of RT-PCR indicated that from a total of 32 samples collected, 12 (41%) were positive by this method. Out of those collected samples, 29 were from goats and 3 were from sheep. Nasal and rectal swabs and blood samples were superior in detection of the PPR virus compared to other tissue samples.
KeywordsPeste des petits ruminants virus Reverse transcriptase polymerase chain reaction Anseba Region Eritrea
The authors are thankful to Mr. Semere Amlesom, Dean of Hamelmalo Agricultural College (HAC), for his encouragement to accomplish this study. We are also thankful to Mr. Efrem Ghebremeskel, Director of the National Animal and Plant Health Laboratory (NAPHL), Asmara, for allowing us to use the laboratory facilities for analyzing and completion of this study. Special thanks to Dr. Sobhi Ahmed Mohammed Khair for his valuable comments and suggestions, and Yodahi Peteros and Yohana Simon for their technical support. Grateful thanks to International Atomic Energy Agency (IAEA) for providing as the necessary diagnostic kits. The authors want also to express their gratitude to the veterinary staff in Anseba Region for their help in the collection of the clinical samples.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
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