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Occurrence and antimicrobial resistance of E. coli non-O157 isolated from beef in Mato Grosso, Brazil

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Abstract

Shiga toxin–producing Escherichia coli (STEC) is an important public health concern pathogen, as it produces two toxins, Stx1 and Stx2, with cytotoxic capacity. In addition, STEC strains are frequently involved in food outbreaks worldwide, leading to public health challenges and economic losses. In this context, the occurrence and antimicrobial resistance profile of the STEC isolated from fresh beef produced in Mato Grosso, Brazil, were estimated. One hundred seven retail beef under vacuum-packaged produced by 13 different slaughterhouses were submitted to microbiological, molecular, and antimicrobial resistance analyses. STEC occurrence in beef was of 4.67%, and five strains presented the stx2 gene. The O111 serogroup, reported in several outbreak cases worldwide, was detected, and other serotypes (O8:H20, O22:H16, and O141:H49) were also isolated. All isolated strains displayed sensitivity to 12 antibiotics, except for two strains, which where streptomycin-resistant. The presence of STEC in retail beef samples indicates public health risks with significant economic impact throughout the retail beef chain.

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Acknowledgments

The authors would like to thank Dr. Rachel Ann Hauser-Davis (Fiocruz) for manuscript revision. The Fiocruz Enterobacteria Laboratory (LABENT), Fundação de Amparo à Pesquisa do Estado de Mato Grosso—FAPEMAT, and the Coordenação de Aperfeiçoamento de Pessoal do Ensino Superior (CAPES).

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Authors and Affiliations

  1. Institute of Chemistry, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-909, Brazil

    Vinicius Silva Castro & Carlos Adam Conte-Junior

  2. Department of Food and Nutrition, Universidade Federal de Mato Grosso, Mato Grosso, 78060-900, Brazil

    Larrayane Albuês Carvalho Teixeira & Eduardo Eustáquio de Souza Figueiredo

  3. National Reference Laboratory for Diagnosis of Enteric Bacteria, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil

    Dália dos Prazeres Rodrigues

  4. Center for Enteric Diseases and Special Infections by Pathogens, Instituto Adolf Lutz, São Paulo, Brazil

    Luis Fernando dos Santos

  5. National Institute of Health Quality Control, Fundação Oswaldo Cruz, Rio de Janeiro, Rio de Janeiro, 21040-900, Brazil

    Carlos Adam Conte-Junior

  6. Department of Food Technology, Faculty of Veterinary Medicine, Universidade Federal Fluminense, Rio de Janeiro, 24230-340, Brazil

    Carlos Adam Conte-Junior

  7. Department of Agronomy and Zootechny, Universidade Federal de Mato Grosso, Mato Grosso, 78060-900, Brazil

    Eduardo Eustáquio de Souza Figueiredo

  8. Federal University of Mato Grosso, Fernando Correa da Costa Avenue, Boa Esperança, Campus Cuiabá, Mato Grosso, Brazil

    Eduardo Eustáquio de Souza Figueiredo

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  1. Vinicius Silva Castro
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Correspondence to Eduardo Eustáquio de Souza Figueiredo.

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Castro, V.S., Teixeira, L.A.C., Rodrigues, D.d. et al. Occurrence and antimicrobial resistance of E. coli non-O157 isolated from beef in Mato Grosso, Brazil. Trop Anim Health Prod 51, 1117–1123 (2019). https://doi.org/10.1007/s11250-018-01792-z

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