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Tropical Animal Health and Production

, Volume 50, Issue 4, pp 907–910 | Cite as

Rapid diagnosis of tuberculosis in dromedary camel (Camelus dromedarius) using lateral flow assay-based kit

  • Rakesh Ranjan
  • Shirish D. Narnaware
  • Kashi Nath
  • R. K. Sawal
  • N. V. Patil
Short Communications

Abstract

Accurate early antemortem diagnosis of tuberculosis in dromedary camels is difficult due to the lack of reliable diagnostic test. The present study aimed to evaluate a lateral flow assay-based kit (rapid assay kit) in tuberculosis diagnosis that employs immuno-chromatographic detection of antibodies in serum, plasma, or whole blood. In a dromedary camel herd comprising 337 animals located at Bikaner, Rajasthan, India, 50 adult weak camels (11 males and 39 females) were tested by applying a single intradermal tuberculin test (SIDT) and rapid assay kit. A total of 14 animals (2 males, 12 females) were found positive in rapid assay. In SIDT, four animals revealed a positive reaction in the neck region and seven animals in the tail base. Another male animal was found SIDT positive but negative in rapid assay; it died after 12 months. Nine rapid assay positive animals died asymptomatically in 1- to 11-month period revealing postmortem tuberculosis lesions that were confirmed by Ziehl-Neelsen staining and histopathology. No tuberculous lesion was evident in the animal found positive in SIDT alone. Results of the present study indicated that serological tests like rapid assay kit can serve as a reliable test for antemortem diagnosis of tuberculosis in dromedary camel.

Keywords

Camel Diagnosis Intradermal tuberculin test Lateral flow assay Tuberculosis 

Notes

Acknowledgements

Authors are thankful to Bobbie Coco, Chembio Diagnostic Systems, Inc. for providing DPP Vet TB assay kits.

Compliance with ethical standards

Statement of animal rights

The manuscript does not contain any experimental trial.

Conflict of interest

The authors declare that they have no competing interest.

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2017

Authors and Affiliations

  1. 1.ICAR-National Research Centre on CamelBikanerIndia

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