Insights into phytase-containing transgenic Lemna minor (L.) as a novel feed additive
- 46 Downloads
This study assessed the effect of supplementation of novel transgenic phytase on growth performance and bone mineralization in Korean native broiler chickens. The experiment was designed using four dietary groups: those with a diet supplemented with (A) recombinant phytase, (B) transgenic phytase from the plant Lemna minor, (C) or wild-type L. minor as well as (D) a control group that was supplemented with commercially available feed. Three hundred 1-day-old Korean native broiler chicks were used and divided into these four dietary treatment groups having three replicates of 25 birds each (n = 75). The results showed increases in growth performance and bone mineralization in Groups B and C; compared with Groups A and D. Hematological analyses revealed notable contrasts in erythrocyte sedimentation rate, red blood cell count, and hemoglobin levels among the experimental groups, whereas no impacts of dietary treatment were observed on total eosinophil, lymphocyte, heterophil, monocyte, and basophil levels. The relative expression profiling of candidate genes showed that the genes involved in growth response, meat quality, and P–Ca metabolism were significantly highly expressed in the phytase-supplemented groups. Hence, it is suggested that dietary supplementation with transgenic phytase plant L. minor for enhancing growth performance is a promising new approach in the broiler feed industry. To the best of our knowledge, we report here the most comprehensive analysis using a broiler model that provides a workable platform for further research on the cost-effective production of feed with different compositions that might be beneficial in the livestock feed industry.
KeywordsBioavailability Broiler chicken Growth performance Gene expression Transgenic phytase
The authors are thankful for support from the Next-Generation Bio-Green 21 Program (No. PJ01117401), Rural Development Administration, Republic of Korea.
DKJ and SK contributed to the design and conception of the project; MG contributed to the design, analysis, and interpretation of data; NS contributed to the evaluation of intellectual content; NK, MG, and DH contributed to the collection, harvesting tissue samples, and mRNA analyses; SSS and JZ contributed to the collection of field samples; TSM, MBK, VPBR, and DKJ contributed to the revision of the manuscript; all authors read and approved the final manuscript.
Compliance with ethical standards
Conflict of interest
The authors do not have any conflict of interest.
- Abioye S, Ige D, Akinremi O, Nyachoti M, Flaten D (2010) Characterizing fecal and manure phosphorus from pigs fed phytase supplemented diets. J Agric Sci 2:3–12Google Scholar
- Ghahri H, Rostami D, Zandiyeh MA, Abbasi RH (2012) The effects of phytase on performance, serum mineral levels, enzyme activities and immune function of broilers fed nutritionally marginal diets. Middle East J Sci Res 11:1481–1490Google Scholar
- Ghosh M, Huynh D, Singh Sodhi S, Sharma N, Kim JH, Kim N, Mongre RK, Park WP, Shin HS, Ko S, Oh S, Choi CW, Oh SJ, Jeong DK (2015a) Impact of a novel phytase derived from Aspergillus nidulans and expressed in transgenic Lemna minor on the performance, mineralization in bone and phosphorous excretion in laying hens. Pak Vet J 35:360–364Google Scholar
- Ghosh M, Sodhi SS, Song KD, Kim JH, Mongre RK, Sharma N, Singh NK, Kim SW, Lee HK, Jeong DK (2015b) Evaluation of body growth and immunity-related differentially expressed genes through deep RNA sequencing in the piglets of Jeju native pig and Berkshire. Anim Genet 46:255–264CrossRefPubMedGoogle Scholar
- Gontia I, Tantwai K, Rajput LPS, Tiwari S (2012) Transgenic plants expressing phytase gene of microbial origin and their prospective application as feed. Food Technol Biotechnol 50:3–10Google Scholar
- Jozefiak D, Ptak A, Kaczmarek S, Mackowiak P, Sassek M, Slominski BA (2010) Multi-carbohydrase and phytase supplementation improves growth performance and liver insulin receptor sensitivity in broiler chickens fed diets containing full-fat rapeseed. Poult Sci 89:1939–1946CrossRefPubMedGoogle Scholar
- Kabir J, Islam MA, Ahammad MU, Howlider MAR (2005) Use of duckweed (Lemna minor) in the diet of broiler. Indian J Anim Res 39:31–35Google Scholar
- McCormick K, Walk CL, Wyatt CL, Adeola O (2017) Phosphorus utilization response of pigs and broiler chickens to diets supplemented with antimicrobials and phytase. Anim Nutri 16:1–8Google Scholar
- Mwale M, Gwaze FR (2013) Characteristics of duckweed and its potential as feed source for chickens reared for meat production: a review. Sci Res Essays 8:689–697Google Scholar