Molecular cloning of Ve promoters from Gossypium barbadense and G. hirsutum and functional analysis in Verticillium wilt resistance
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Verticillium wilt caused by Verticillium dahliae is the most serious disease in cotton. Ve gene isolated from Gossypium barbadense is one of the resistant genes related to Verticillium wilt. In this study, we analyzed Ve expression pattern in different resistant cotton cultivars under V. dahliae stress, and isolated the upstream promoters of Ve from G. barbadense and G. hirsutum. We found that the expression of Ve gene in upland cottons increased significantly at 6 hpi and decreased at 12 hpi, however, continued to increase in Sea Island cotton under V. dahliae stress. Through detecting the GUS activity in the transgenic pVe::GUS Arabidopsis, we discovered that the upstream from − 979 to − 1 bp was the important region of Ve gene promoter. The activity of Ve gene promoters was higher in the roots than that in other tissues in Arabidopsis, and the promoters could be induced by V. dahliae. Moreover, the activity of promoter in G. barbadense was significantly higher than that in G. hirsutum, and the promoter of Ve in G. barbadense contained regulatory elements S1FBOXSORPS1L21 and S1FSORPL21 compared to that in G. hirsutum. We inferred that the regulatory elements S1FBOXSORPS1L21 and S1FSORPL21 were related to the Ve gene expression. This provides new insights into dissecting the defense mechanisms of Ve against V. dahliae in cotton.
KeywordsPromoter Ve gene Verticillium wilt G. barbadense G. hirsutum
This work was supported by the National Natural Science Foundation of China (No. 31171597) and Hundreds of Innovative Talents Project of Hebei Province (14226308D).
XW designed the research. LL and WW performed the experiments. LL, JY, YZ and GZ performed the data analysis. LL prepared the manuscript. XW and ZM revised the manuscript. All authors approved the final version of the manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Long L, Zhao JR, Xu FC, Yang WW, Liao P, Gao Y, Gao W, Song CP (2018) Silencing of GbANS reduces cotton resistance to Vercillium dahliae through decreased ROS scavenging during the pathogen invasion process. Plant Cell Tissue Organ Cult. https://doi.org/10.1007/s11240-018-1457-y CrossRefGoogle Scholar
- Qi WY, Zhang YJ, Zhang TZ, Chen JY, Dai XF (2012) Studies on the methods for cotton resistant breeding to Verticillium wilt by the screen of disease nursery and molecular marker-assisted selection. Mol Plant Breed 5:607–612 (in Chinese)Google Scholar
- Wang XF, Ma ZY (2002) A new method for identification of cotton Verticillium wilt resistance. Cotton Sci 14:231–233 (in Chinese)Google Scholar
- Wang GN, Zhao GY, Yue XW, Li ZK, Zhang Y, Zhang GY, Wu LQ, Wang XF, Ma ZY (2012) Pathogenicity and ISSR genetic differentiation of Verticillium dahliae isolates from cotton growing areas of Hebei province. Cotton Sci 24:348–357 (in Chinese)Google Scholar
- Yang Y, Ling X, Chen T, Cai L, Liu T, Wang J, Fan X, Ren Y, Yuan H, Zhu W, Zhang B, Ma D-P (2014) A cotton Gbvdr5 gene encoding a leucine-rich-repeat receptor-like protein confers resistance to Verticillium dahliae in transgenic Arabidopsis and upland cotton. Plant Mol Biol Rep 33:987–1001CrossRefGoogle Scholar
- Zhang X, Zhang S, Li Q, Chen B, Chen J, Yao Y (1981) Identification of Verticillium wilt pathogen of cotton in China. Acta Phytopathol Sin 11:149–156 (in Chinese)Google Scholar
- Zhang CY, Wang XF, Zhang GY, Wu LQ, Chi JN, Li ZK, Ma ZY (2010) ESTs analysis of suppression subtractive hybridization library from upland cotton resistant cultivar infected by Verticillium dahliae. Cotton Sci 22:17–22 (in Chinese)Google Scholar
- Zhang BJ, Zhang HP, Chen QZ, Tang N, Wang LK, Wang RF, Zhang BL (2016) Molecular cloning and analysis of a receptor-like promoter of Gbvdr3 gene in sea island cotton. Genet Mol Res 15(2):gmr.15028636Google Scholar