Skip to main content
SpringerLink
Log in

Molecular cloning of Ve promoters from Gossypium barbadense and G. hirsutum and functional analysis in Verticillium wilt resistance

  • Original Article
  • Published:
Plant Cell, Tissue and Organ Culture (PCTOC) Aims and scope Submit manuscript

Abstract

Verticillium wilt caused by Verticillium dahliae is the most serious disease in cotton. Ve gene isolated from Gossypium barbadense is one of the resistant genes related to Verticillium wilt. In this study, we analyzed Ve expression pattern in different resistant cotton cultivars under V. dahliae stress, and isolated the upstream promoters of Ve from G. barbadense and G. hirsutum. We found that the expression of Ve gene in upland cottons increased significantly at 6 hpi and decreased at 12 hpi, however, continued to increase in Sea Island cotton under V. dahliae stress. Through detecting the GUS activity in the transgenic pVe::GUS Arabidopsis, we discovered that the upstream from − 979 to − 1 bp was the important region of Ve gene promoter. The activity of Ve gene promoters was higher in the roots than that in other tissues in Arabidopsis, and the promoters could be induced by V. dahliae. Moreover, the activity of promoter in G. barbadense was significantly higher than that in G. hirsutum, and the promoter of Ve in G. barbadense contained regulatory elements S1FBOXSORPS1L21 and S1FSORPL21 compared to that in G. hirsutum. We inferred that the regulatory elements S1FBOXSORPS1L21 and S1FSORPL21 were related to the Ve gene expression. This provides new insights into dissecting the defense mechanisms of Ve against V. dahliae in cotton.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  Google Scholar 

  • Chen XL, Song RT, Yu MY, Sui JM, Wang JS, Qiao LX (2015) Cloning and functional analysis of the chitinase gene promoter in peanut. Genet Mol Res 14:12710–12722

    Article  CAS  Google Scholar 

  • Chen T, Kan J, Yang Y, Ling X, Chang Y, Zhang B (2016) A Ve homologous gene from Gossypium barbadense, Gbvdr3, enhances the defense response against Verticillium dahliae. Plant Physiol Biochem 98:101–111

    Article  CAS  Google Scholar 

  • Clough SJ, Bent AF (1998) Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J 16(6):735–743

    Article  CAS  Google Scholar 

  • Covey SN, Lomonossoff GP, Hull R (1981) Characterisation of cauliflower mosaic virus DNA sequences which encode major polyadenylated transcripts. Nucleic Acids Res 9:6735–6748

    Article  CAS  Google Scholar 

  • Edwards JW, Walker EL, Coruzzi GM (1990) Cell-specific expression in transgenic plants reveals nonoverlapping roles for chloroplast and cytosolic glutamine synthetase. Proc Natl Acad Sci USA 87:3459–3463

    Article  CAS  Google Scholar 

  • Fradin EF, Zhang Z, Juarez Ayala JC, Castroverde CD, Nazar RN, Robb J, Liu CM, Thomma BP (2009) Genetic dissection of Verticillium wilt resistance mediated by tomato Ve. Plant Physiol 150:320–332

    Article  CAS  Google Scholar 

  • Fradin EF, Abd-El-Haliem A, Masini L, van den Berg GC, Joosten MH, Thomma BP (2011) Interfamily transfer of tomato Ve1 mediates Verticillium resistance in Arabidopsis. Plant Physiol 156:2255–2265

    Article  CAS  Google Scholar 

  • Gururani MA, Venkatesh J, Upadhyaya CP, Nookaraju A, Pandey SK, Park SW (2012) Plant disease resistance genes: current status and future directions. Physiol Mol Plant Pathol 78:51–65

    Article  CAS  Google Scholar 

  • Kawchuk LM, Hachey J, Lynch DR, Kulcsar F, van Rooijen G, Waterer DR, Robertson A, Kokko E, Byers R, Howard RJ, Fischer R, Prufer D (2001) Tomato Ve disease resistance genes encode cell surface-like receptors. Proc Natl Acad Sci USA 98:6511–6515

    Article  CAS  Google Scholar 

  • Long L, Zhao JR, Xu FC, Yang WW, Liao P, Gao Y, Gao W, Song CP (2018) Silencing of GbANS reduces cotton resistance to Vercillium dahliae through decreased ROS scavenging during the pathogen invasion process. Plant Cell Tissue Organ Cult. https://doi.org/10.1007/s11240-018-1457-y

    Article  Google Scholar 

  • Luo H, Song F, Goodman RM, Zheng Z (2005) Up-regulation of OsBIHD1, a rice gene encoding BELL homeodomain transcriptional factor, in disease resistance responses. Plant Biol 7:459–468

    Article  CAS  Google Scholar 

  • Porebski S, Bailey LG, Baum BR (1997) Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Mol Biol Rep 15:8–15

    Article  CAS  Google Scholar 

  • Qi WY, Zhang YJ, Zhang TZ, Chen JY, Dai XF (2012) Studies on the methods for cotton resistant breeding to Verticillium wilt by the screen of disease nursery and molecular marker-assisted selection. Mol Plant Breed 5:607–612 (in Chinese)

    Google Scholar 

  • Redman J, Whitcraft J, Johnson C, Arias J (2002) Abiotic and biotic stress differentially stimulate as-1 element activity in Arabidopsis. Plant Cell Rep 21:180–185

    Article  CAS  Google Scholar 

  • Shi W, Liu D, Hao L, Wu CA, Guo X, Li H (2014) GhWRKY39, a member of the WRKY transcription factor family in cotton, has a positive role in disease resistance and salt stress tolerance. Plant Cell Tissue Organ Cult 118:17–32

    Article  CAS  Google Scholar 

  • Wang XF, Ma ZY (2002) A new method for identification of cotton Verticillium wilt resistance. Cotton Sci 14:231–233 (in Chinese)

    Google Scholar 

  • Wang GN, Zhao GY, Yue XW, Li ZK, Zhang Y, Zhang GY, Wu LQ, Wang XF, Ma ZY (2012) Pathogenicity and ISSR genetic differentiation of Verticillium dahliae isolates from cotton growing areas of Hebei province. Cotton Sci 24:348–357 (in Chinese)

    CAS  Google Scholar 

  • Wu LZ, Wang XF, Zhang Y, Li XH, Zhang GY, Wu LQ, Li ZK, Ma ZY (2014) Function of acid insoluble lignin and GhLaccase in cotton resistance to Verticillium wilt. Acta Agron Sin 40:1157–1163 (in Chinese)

    Article  CAS  Google Scholar 

  • Wu JD, Jiang CP, Zhu HS, Jiang HY, Cheng BJ, Zhu SW (2015) Cloning and functional analysis of the promoter of a maize starch synthase III gene (ZmDULL1). Genet Mol Res 14:5468–5479

    Article  CAS  Google Scholar 

  • Xiao K, Liu J, Dewbre G, Harrison M, Wang ZY (2006) Isolation and characterization of root-specific phosphate transporter promoters from Medicago truncatula. Plant Biol 8:439–449

    Article  CAS  Google Scholar 

  • Yang Y, Ling X, Chen T, Cai L, Liu T, Wang J, Fan X, Ren Y, Yuan H, Zhu W, Zhang B, Ma D-P (2014) A cotton Gbvdr5 gene encoding a leucine-rich-repeat receptor-like protein confers resistance to Verticillium dahliae in transgenic Arabidopsis and upland cotton. Plant Mol Biol Rep 33:987–1001

    Article  Google Scholar 

  • Yang Y, Chen T, Ling X, Ma Z (2017) Gbvdr6, a gene encoding a receptor-like protein of cotton (Gossypium barbadense), confers resistance to Verticillium Wilt in Arabidopsis and upland cotton. Front Plant Sci 8:2272

    Article  Google Scholar 

  • Zhang X, Zhang S, Li Q, Chen B, Chen J, Yao Y (1981) Identification of Verticillium wilt pathogen of cotton in China. Acta Phytopathol Sin 11:149–156 (in Chinese)

    Google Scholar 

  • Zhang CY, Wang XF, Zhang GY, Wu LQ, Chi JN, Li ZK, Ma ZY (2010) ESTs analysis of suppression subtractive hybridization library from upland cotton resistant cultivar infected by Verticillium dahliae. Cotton Sci 22:17–22 (in Chinese)

    CAS  Google Scholar 

  • Zhang Y, Wang X, Yang S, Chi J, Zhang G, Ma Z (2011) Cloning and characterization of a Verticillium wilt resistance gene from Gossypium barbadense and functional analysis in Arabidopsis thaliana. Plant Cell Rep 30:2085–2096

    Article  CAS  Google Scholar 

  • Zhang B, Yang Y, Chen T, Yu W, Liu T, Li H, Fan X, Ren Y, Shen D, Liu L, Dou D, Chang Y (2012) Island cotton Gbve1 gene encoding a receptor-like protein confers resistance to both defoliating and non-defoliating isolates of Verticillium dahliae. PLoS ONE 7:e51091

    Article  CAS  Google Scholar 

  • Zhang Y, Wang XF, Ding ZG, Ma Q, Zhang GR, Zhang SL, Li ZK, Wu LQ, Zhang GY, Ma ZY (2013) Transcriptome profiling of Gossypium barbadense inoculated with Verticillium dahliae provides a resource for cotton improvement. BMC Genomics 14:637

    Article  CAS  Google Scholar 

  • Zhang JF, Fang H, Zhou HP, Sanogo S, Ma ZY (2014) Genetics, breeding, and marker-assisted selection for Verticillium wilt resistance in cotton. Crop Sci 54:1289–1303

    Article  Google Scholar 

  • Zhang BJ, Zhang HP, Chen QZ, Tang N, Wang LK, Wang RF, Zhang BL (2016) Molecular cloning and analysis of a receptor-like promoter of Gbvdr3 gene in sea island cotton. Genet Mol Res 15(2):gmr.15028636

    Google Scholar 

  • Zhou DX, Li YF, Rocipon M, Mache R (1992) Sequence-specific interaction between S1F, a spinach nuclear factor, and a negative cis-element conserved in plastid-related genes. J Biol Chem 267:23515–23519

    CAS  PubMed  Google Scholar 

  • Zuo JR, Chua NH (2000) Chemical-inducible systems for regulated expression of plant genes. Curr Opin Biotechnol 11:146–151

    Article  CAS  Google Scholar 

  • Zuo KJ, Qin J, Zhao JY, Ling H, Zhang LD, Cao YF, Tang KX (2007) Over-expression GbERF2 transcription factor in tobacco enhances brown spots disease resistance by activating expression of downstream genes. Gene 391:80–90

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 31171597) and Hundreds of Innovative Talents Project of Hebei Province (14226308D).

Author information

Author notes

  1. Lixia Liu and Weiqiao Wang have contributed equally to this work.

Authors and Affiliations

  1. North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Key Laboratory for Crop Germplasm Resources of Hebei, Hebei Agricultural University, Baoding, 071001, China

    Lixia Liu, Weiqiao Wang, Jun Yang, Yan Zhang, Guiyin Zhang, Zhiying Ma & Xingfen Wang

Authors
  1. Lixia Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Weiqiao Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jun Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yan Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Guiyin Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Zhiying Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Xingfen Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

XW designed the research. LL and WW performed the experiments. LL, JY, YZ and GZ performed the data analysis. LL prepared the manuscript. XW and ZM revised the manuscript. All authors approved the final version of the manuscript.

Corresponding author

Correspondence to Xingfen Wang.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Communicated by Maria Margarida Oliveira.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Liu, L., Wang, W., Yang, J. et al. Molecular cloning of Ve promoters from Gossypium barbadense and G. hirsutum and functional analysis in Verticillium wilt resistance. Plant Cell Tiss Organ Cult 135, 535–544 (2018). https://doi.org/10.1007/s11240-018-1485-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11240-018-1485-7

Keywords

Search

Navigation