In vitro propagation of an endemic and endangered medicinal plant Notopterygium incisum
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An efficient system in vitro propagation for Notopterygium incisum Ting ex H. T. Chang, an endemic and endangered medicinal plant, was established to address increased demand and germplasm conservation goals. Optimum response in callus induction (CI) was observed on Murashige and Skoog (MS) medium supplemented with 1.5 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.2 mg/l 6-benzylaminopurine (BAP), which the induction rate and growth of callus were 84.44% and 0.67 g respectively. The highest shoot regeneration frequency (76.97%) and maximum number of shoots (3.6 shoots per callus) were achieved on MS medium with 1.5 mg/l BAP and 0.2 mg/l naphthalene acetic acid (NAA). Half-strength MS medium supplemented with 0.6 mg/l indole-3-butyric acid (IBA) was determined to be the best rooting medium, resulting in the maximum number of roots (18.6 roots per shoot) and the highest rooting frequency (92.28%). An approximate 83.8% survival rate among the regenerated plantlets was recorded after they were transplanted in the field at an altitude of 3200 m. An HPLC analysis showed that the content of two main chemical constituents, notopterol and isoimperatorin, in the rhizomes of 3-year-old regenerated plantlets was higher (3.84 mg/g and 4.05 mg/g, respectively) than that in commercially marketed crude drugs. This first report of complete regeneration in vitro could provide an alternative method for the rapid, large-scale production and conservation of this valuable, rare, and endangered medicinal plant.
KeywordsNotopterygium incisum Ting ex H. T. Chang In vitro Plantlet production HPLC
High performance liquid chromatography
Naphthalene acetic acid
Plant growth regulators
This work was supported by the National Natural Science Foundation of China (Grant No. 81473311) and National Key Research and Development Program of China (Grant No. 2017YFC1700705).
SYJ conceived and designed the study and in compiling the manuscript. WTZ, XJC and ZY carried out the in vitro propagation research work and wrote the manuscript. JZD and HBS analyzed the main chemical constituents of rhizomes in vitro. HS and HLW carried out data extraction and analysis. All authors read and approved the final manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Ashwini AM, Ramakrishnaiah H, Manohar SH, Majumdar M (2015) An efficient multiple shoot induction and genetic fidelity assessment of Exacum bicolor Roxb., an endemic and endangered medicinal plant. In Vitro Cell Dev Biol Plant 51:659–668. https://doi.org/10.1007/s11627-015-9726-5 CrossRefGoogle Scholar
- Bose B, Kumaria S, Choudhury H, Tandon P (2016) Assessment of genetic homogeneity and analysis of phytomedicinal potential in micropropagated plants of Nardostachys jatamansi, a critically endangered, medicinal plant of alpine Himalayas. Plant Cell, Tissue Organ Cult (PCTOC) 124(2):331–349CrossRefGoogle Scholar
- Chinese Pharmacopoeia Commission (2015) In: Pharmacopoeia of the People’s Republic of China, vol I. Medical Science Publishing House of China, BeijingGoogle Scholar
- Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x CrossRefGoogle Scholar
- Paramageetham Ch, Prasad BG, Rao JVS (2004) Somatic embryogenesis in Centella asiatica L. an important medicinal and neutraceutical plant of India. Plant Cell Tissue Organ Cult 79:19–24. https://doi.org/10.1023/B:TICU.0000049427.27937.d2 CrossRefGoogle Scholar
- Pu FT, Wang PL, Zheng ZH, Wang YP (2000) A reclassification of Notopterygium Boissieu. Acta Phytotaxonom Sinica 38(5):430–436Google Scholar
- Shi J, Jiang SY, Ma XJ, Sun H, Zhou Y (2007) Studies on seeds germination and seedlings growth of Notopterygium incisum. China J Chin Mater Med 32:1841–1844Google Scholar
- Wang S, Xie Y (2004) In: China special red list, vol I. China Higher Education Press, BeijingGoogle Scholar
- Zhou Y, Jiang SY, Sun H et al (2007) Quantitative analysis of volatile oils and isoimperatorin in rhizoma et Radix notopterygii. China J Chin Mater Med 32(7):566–569Google Scholar