Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 135, Issue 3, pp 559–563 | Cite as

In vitro propagation of an endemic and endangered medicinal plant Notopterygium incisum

  • Wen-Tao Zhu
  • Jiu-Zhen Du
  • Hong-Bing Sun
  • Shun-Yuan JiangEmail author
  • Xue-Jun Chen
  • Hui Sun
  • Yi Zhou
  • Hong-Lan Wang
Research Note


An efficient system in vitro propagation for Notopterygium incisum Ting ex H. T. Chang, an endemic and endangered medicinal plant, was established to address increased demand and germplasm conservation goals. Optimum response in callus induction (CI) was observed on Murashige and Skoog (MS) medium supplemented with 1.5 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.2 mg/l 6-benzylaminopurine (BAP), which the induction rate and growth of callus were 84.44% and 0.67 g respectively. The highest shoot regeneration frequency (76.97%) and maximum number of shoots (3.6 shoots per callus) were achieved on MS medium with 1.5 mg/l BAP and 0.2 mg/l naphthalene acetic acid (NAA). Half-strength MS medium supplemented with 0.6 mg/l indole-3-butyric acid (IBA) was determined to be the best rooting medium, resulting in the maximum number of roots (18.6 roots per shoot) and the highest rooting frequency (92.28%). An approximate 83.8% survival rate among the regenerated plantlets was recorded after they were transplanted in the field at an altitude of 3200 m. An HPLC analysis showed that the content of two main chemical constituents, notopterol and isoimperatorin, in the rhizomes of 3-year-old regenerated plantlets was higher (3.84 mg/g and 4.05 mg/g, respectively) than that in commercially marketed crude drugs. This first report of complete regeneration in vitro could provide an alternative method for the rapid, large-scale production and conservation of this valuable, rare, and endangered medicinal plant.


Notopterygium incisum Ting ex H. T. Chang In vitro Plantlet production HPLC 



2,4-Dichlorophenoxyacetic acid




Callus induction


Fresh weight


High performance liquid chromatography


Indole-3-butyric acid




Naphthalene acetic acid


Plant growth regulators



This work was supported by the National Natural Science Foundation of China (Grant No. 81473311) and National Key Research and Development Program of China (Grant No. 2017YFC1700705).

Author contributions

SYJ conceived and designed the study and in compiling the manuscript. WTZ, XJC and ZY carried out the in vitro propagation research work and wrote the manuscript. JZD and HBS analyzed the main chemical constituents of rhizomes in vitro. HS and HLW carried out data extraction and analysis. All authors read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Wen-Tao Zhu
    • 1
  • Jiu-Zhen Du
    • 1
  • Hong-Bing Sun
    • 1
  • Shun-Yuan Jiang
    • 1
    Email author
  • Xue-Jun Chen
    • 2
  • Hui Sun
    • 3
  • Yi Zhou
    • 1
  • Hong-Lan Wang
    • 1
  1. 1.Sichuan Academy of Chinese Medical SciencesChengduChina
  2. 2.YATAS, YNTCKunmingChina
  3. 3.Sichuan UniversityChengduChina

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