Promotion of shoot regeneration of Swertia chirata by biosynthesized silver nanoparticles and their involvement in ethylene interceptions and activation of antioxidant activity

Original Article
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Abstract

Chemically synthesized silver nanoparticles (AgNP) have been assessed on plant tissue cultures but the impact of biologically synthesized AgNP fabricated by different phytoconstituents has not been sufficiently investigated. In this study biogenic AgNP prepared from leaf extract of Swertia chirata was utilized to address the problems associated with conservation of endangered medicinal plant through plant tissue culture. Endangered as well as economically important medicinal plant considered for this analysis was the same plant S. chirata itself. Excessive deforestation and exploration of this plant had led to complete eradication of the natural reservoir. Thus in vitro plant tissue propagation had attained much importance for maintaining the plant species. Nano-sized particles of diameter 20 nm encapped by different phytochemicals applied on regenerating shoot cultures showed enhanced shoot induction and proliferation. Other forms of silver like AgNO3 (SN) and Ag2S2O3 (STS) showed improved regeneration in comparison to control samples. Ethylene precursors like 1-aminocyclopropane-1-carboxylic acid (ACC) and 2-chloroethylphosphonic acid (CEPA) downregulated the regeneration process considerably. Reactive oxygen species (ROS) status of the treated cultures were evaluated considering hydrogen peroxide and malondialdehyde (MDA) content in treated cells as wells as their antioxidant enzyme activity. Combined manipulation and coordination of ethylene evolution, maintenance of ROS balance and better bio-acceptance of AgNP were responsible for improvement in shoot regeneration of the plant. Phytoencapsulated and nano-dimensioned Ag was capable of changing the chemical reactions of different regulating players of plant regeneration. These findings will facilitate the understanding and future utilization of biofabricated AgNP in agriculture and plant sciences.

Keywords

Silver nanoparticles Plant tissue culture Shoot regeneration Ethylene Antioxidant activity 

Abbreviations

AgNP

Silver nanoparticles

SN

Silver nitrate

STS

Silver thiosulphate

ACC

1-Aminocyclopropane-1-carboxylic acid

CEPA

2-Chloroethylphosphonic acid

ROS

Reactive oxygen species

MDA

Malondialdehyde

SPR

Surface plasmon resonance

MS

Murashige and Skoog medium

BAP

6-Benzylaminopurine

CC

Cobalt chloride

NaTS

Sodium thiosulphate

TCA

Trichloroacetic acid

TBA

Thiobarbituric acid

SOD

Superoxide dismutase

CAT

Catalase

POX

Peroxidase

APX

Ascorbate peroxidase

GR

Glutathione reductase

PMSF

Phenylmethylsulfonyl fluoride

PVP

Polyvinylpyrrolidone

EDTA

Ethylene diaminetetraacetic acid

BSA

Bovine serum albumin

NBT

Nitro blue tetrazolium chloride

GSSG

Oxidized glutathione

NADPH

Nicotinamide adenine dinucleotide phosphate reduced

ANOVA

One-way analysis of variance

DMRT

Duncan’s multiple range test

LSD

Least significant difference

TEM

Transmission electron microscopy

FTIR

Fourier-transform infrared spectroscopy.

Notes

Acknowledgements

We gratefully acknowledge the financial support from the Department of Agriculture and Food Engineering, Indian Institute of Technology, Kharagpur.

Author contributions

All authors listed, have made considerable, direct and intellectual contribution to the work, and approved it for publication.

Compliance with ethical standards

Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Supplementary material

11240_2018_1423_MOESM1_ESM.docx (18 kb)
Supplementary material 1 (DOCX 17 KB)

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© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Agriculture and Food EngineeringIndian Institute of TechnologyKharagpurIndia

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