Abstract
Chemically synthesized silver nanoparticles (AgNP) have been assessed on plant tissue cultures but the impact of biologically synthesized AgNP fabricated by different phytoconstituents has not been sufficiently investigated. In this study biogenic AgNP prepared from leaf extract of Swertia chirata was utilized to address the problems associated with conservation of endangered medicinal plant through plant tissue culture. Endangered as well as economically important medicinal plant considered for this analysis was the same plant S. chirata itself. Excessive deforestation and exploration of this plant had led to complete eradication of the natural reservoir. Thus in vitro plant tissue propagation had attained much importance for maintaining the plant species. Nano-sized particles of diameter 20 nm encapped by different phytochemicals applied on regenerating shoot cultures showed enhanced shoot induction and proliferation. Other forms of silver like AgNO3 (SN) and Ag2S2O3 (STS) showed improved regeneration in comparison to control samples. Ethylene precursors like 1-aminocyclopropane-1-carboxylic acid (ACC) and 2-chloroethylphosphonic acid (CEPA) downregulated the regeneration process considerably. Reactive oxygen species (ROS) status of the treated cultures were evaluated considering hydrogen peroxide and malondialdehyde (MDA) content in treated cells as wells as their antioxidant enzyme activity. Combined manipulation and coordination of ethylene evolution, maintenance of ROS balance and better bio-acceptance of AgNP were responsible for improvement in shoot regeneration of the plant. Phytoencapsulated and nano-dimensioned Ag was capable of changing the chemical reactions of different regulating players of plant regeneration. These findings will facilitate the understanding and future utilization of biofabricated AgNP in agriculture and plant sciences.
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Abbreviations
- AgNP:
-
Silver nanoparticles
- SN:
-
Silver nitrate
- STS:
-
Silver thiosulphate
- ACC:
-
1-Aminocyclopropane-1-carboxylic acid
- CEPA:
-
2-Chloroethylphosphonic acid
- ROS:
-
Reactive oxygen species
- MDA:
-
Malondialdehyde
- SPR:
-
Surface plasmon resonance
- MS:
-
Murashige and Skoog medium
- BAP:
-
6-Benzylaminopurine
- CC:
-
Cobalt chloride
- NaTS:
-
Sodium thiosulphate
- TCA:
-
Trichloroacetic acid
- TBA:
-
Thiobarbituric acid
- SOD:
-
Superoxide dismutase
- CAT:
-
Catalase
- POX:
-
Peroxidase
- APX:
-
Ascorbate peroxidase
- GR:
-
Glutathione reductase
- PMSF:
-
Phenylmethylsulfonyl fluoride
- PVP:
-
Polyvinylpyrrolidone
- EDTA:
-
Ethylene diaminetetraacetic acid
- BSA:
-
Bovine serum albumin
- NBT:
-
Nitro blue tetrazolium chloride
- GSSG:
-
Oxidized glutathione
- NADPH:
-
Nicotinamide adenine dinucleotide phosphate reduced
- ANOVA:
-
One-way analysis of variance
- DMRT:
-
Duncan’s multiple range test
- LSD:
-
Least significant difference
- TEM:
-
Transmission electron microscopy
- FTIR:
-
Fourier-transform infrared spectroscopy.
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We gratefully acknowledge the financial support from the Department of Agriculture and Food Engineering, Indian Institute of Technology, Kharagpur.
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Saha, N., Dutta Gupta, S. Promotion of shoot regeneration of Swertia chirata by biosynthesized silver nanoparticles and their involvement in ethylene interceptions and activation of antioxidant activity. Plant Cell Tiss Organ Cult 134, 289–300 (2018). https://doi.org/10.1007/s11240-018-1423-8
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DOI: https://doi.org/10.1007/s11240-018-1423-8