Promotion of shoot regeneration of Swertia chirata by biosynthesized silver nanoparticles and their involvement in ethylene interceptions and activation of antioxidant activity
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Chemically synthesized silver nanoparticles (AgNP) have been assessed on plant tissue cultures but the impact of biologically synthesized AgNP fabricated by different phytoconstituents has not been sufficiently investigated. In this study biogenic AgNP prepared from leaf extract of Swertia chirata was utilized to address the problems associated with conservation of endangered medicinal plant through plant tissue culture. Endangered as well as economically important medicinal plant considered for this analysis was the same plant S. chirata itself. Excessive deforestation and exploration of this plant had led to complete eradication of the natural reservoir. Thus in vitro plant tissue propagation had attained much importance for maintaining the plant species. Nano-sized particles of diameter 20 nm encapped by different phytochemicals applied on regenerating shoot cultures showed enhanced shoot induction and proliferation. Other forms of silver like AgNO3 (SN) and Ag2S2O3 (STS) showed improved regeneration in comparison to control samples. Ethylene precursors like 1-aminocyclopropane-1-carboxylic acid (ACC) and 2-chloroethylphosphonic acid (CEPA) downregulated the regeneration process considerably. Reactive oxygen species (ROS) status of the treated cultures were evaluated considering hydrogen peroxide and malondialdehyde (MDA) content in treated cells as wells as their antioxidant enzyme activity. Combined manipulation and coordination of ethylene evolution, maintenance of ROS balance and better bio-acceptance of AgNP were responsible for improvement in shoot regeneration of the plant. Phytoencapsulated and nano-dimensioned Ag was capable of changing the chemical reactions of different regulating players of plant regeneration. These findings will facilitate the understanding and future utilization of biofabricated AgNP in agriculture and plant sciences.
KeywordsSilver nanoparticles Plant tissue culture Shoot regeneration Ethylene Antioxidant activity
Reactive oxygen species
Surface plasmon resonance
Murashige and Skoog medium
Ethylene diaminetetraacetic acid
Bovine serum albumin
Nitro blue tetrazolium chloride
Nicotinamide adenine dinucleotide phosphate reduced
One-way analysis of variance
Duncan’s multiple range test
Least significant difference
Transmission electron microscopy
Fourier-transform infrared spectroscopy.
We gratefully acknowledge the financial support from the Department of Agriculture and Food Engineering, Indian Institute of Technology, Kharagpur.
All authors listed, have made considerable, direct and intellectual contribution to the work, and approved it for publication.
Compliance with ethical standards
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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