Statistical optimization for enhanced bacoside A production in plant cell cultures of Bacopa monnieri
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The bioactive compound, bacoside A, has immense importance for the treatment of memory disorders and Alzheimer’s disease. Due to the growing commercial interest in the herb, Bacopa monnieri, it has been listed as highly endangered species. The present study was aimed at enhancing the production of bacoside A using an alternative technology of plant cell suspension culture. Initial experiments of docking simulations using bacoside A showed good inhibition of acetyl cholinesterase (binding energy value of − 20 kcal/mol), when comparison was made with other phytocompounds and the synthetic drug for Alzheimer’s disease. In vitro experiments established that B. monnieri cell suspension culture can be developed in Murashige and Skoog medium containing containing 0.1 mg/L benzylaminopurine and 0.5 mg/L naphthalene acetic acid. Plackett–Burman studies predicted that the most effective factors for maximum biomass production were inoculum size (t-value of 4.87), sucrose concentration (t-value of 0.25) and KH2PO4 concentration (t-value of 0.007). The nitrate to ammonium ratio (t-value of − 0.42) did not have significant effect on the cell suspension biomass. The optimum concentration of the crucial variables obtained from a central composite design were—inoculum size of 2 g/L, sucrose concentration of 30 g/L and KH2PO4 concentration of 1.24 mM in one-sixth strength MS medium. The best model for optimum production of biomass and bacoside A was experimentally verified and the correlation between the predicted and actual values was found to be 99% for biomass and 94% for bacoside A production. The experimental results have been discussed in the present work.
KeywordsBacopa monnieri Benzylaminopurine Naphthalene acetic acid Bacoside A Central composite design
The authors are thankful for the elite plant material supply from CIMAP Lucknow. The financial support by MHRD (India) for pursuing PhD (J. Leonard) and M.Tech (Bishwanath Seth) is acknowledged by two of the authors. The authors also thankfully acknowledge Life Science department (NIT Rourkela) for providing the HPLC facility which was funded by DST (FIST), India [File number: SR/FST/LSI-025/2014].
The docking study, development of cell culture, standardization of HPLC method and PB studies have been performed by JL. BS performed the RSM experiments, experimental validation and analysis of results. BBS and VRS were the collaborators and provided their resources for carrying out this work. NP has been the thesis supervisor for JL and BS in this research work.
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Conflict of interest
The authors declare that they have no conflict of interest.
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