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Stable, fertile somatic hybrids between Sinapis alba and Brassica juncea show resistance to Alternaria brassicae and heat stress

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Abstract

Wild relatives of Brassica are a rich reservoir of genes that are invaluable for the improvement of cultivated species. Sinapis alba is a close relative of crop Brassicas that possesses several desirable traits such as tolerance to Alternaria black spot disease, heat stress, insect pests and nematodes. This study is aimed at developing and characterizing hybrids between Brassica juncea and S. alba with the ultimate goal of transferring genes for tolerance to Alternaria brassicae and heat stress, the traits that are lacking in cultivated Brassica. We generated three hybrids between B. juncea and S. alba through protoplast fusion. The hybridity was confirmed through cytology and molecular markers. While two of the hybrids were symmetric, the third one was asymmetric and had greater resemblance to B. juncea. Hybrids showed some characteristic features of the parents and were fully male and female fertile and also set seeds upon back crossing with the parent species. In vitro leaf assay and field inoculation studies revealed that the hybrids are highly resistant to A. brassicae. Besides, hybrids set seeds at temperature of > 38 °C when parents failed to produce seeds indicating that hybrids possess heat tolerance. These stable hybrids provide a reliable genetic resource for transfer of genes from S. alba into cultivated Brassica species.

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Acknowledgements

P Kumari is supported by a financial assistance from the Science and Engineering Research Board, Govt. of India. YSS (SR/LS/068/2010).

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Authors and Affiliations

  1. ICAR-National Research Centre on Plant Biotechnology, Pusa Campus, New Delhi, 110012, India

    Preetesh Kumari, Deepak Singh Bisht & Shripad R. Bhat

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  1. Preetesh Kumari
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  2. Deepak Singh Bisht
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  3. Shripad R. Bhat
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Contributions

PK- conduct of experiments, collection of data and analysis, preparation of draft manuscript; DSB- molecular analysis; SRB- planning and conduct of experiments, analysis and interpretation of data, manuscript writing and finalization.

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Correspondence to Shripad R. Bhat.

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Communicated by T. Winkelmann.

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Kumari, P., Bisht, D.S. & Bhat, S.R. Stable, fertile somatic hybrids between Sinapis alba and Brassica juncea show resistance to Alternaria brassicae and heat stress. Plant Cell Tiss Organ Cult 133, 77–86 (2018). https://doi.org/10.1007/s11240-017-1362-9

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  • DOI: https://doi.org/10.1007/s11240-017-1362-9

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