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Cloning and characterization of DoMYC2 from Dendrobium officinale

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Abstract

MYC proteins, a group of transcription factors belonging to bHLH super family, play important roles in regulation of metabolism and plant growth. In this study, a novel MYC gene, DoMYC2 (GeneBank accession KJ649471), was isolated from Dendrobium officinale. The full length of DoMYC2 cDNA contains 2351 bp with an open reading frame of 1971 bp encoding 656 amino acids. BlastP analyses showed that its amino acid sequence was homologous with typical MYC proteins in other plant species. Furthermore, expression of DoMYC2 could be induced by MeJA and chitosan, respectively. Overexpression of DoMYC2 led to a decrease in transcript levels of AtHMGR2, AtFPS1 and AtFPS2 in Arabidopsis. Moreover, DoMYC2 was able to bind to the E-box cis-elements in yeast. These results suggest that DoMYC2 participate in terpenoid indole alkaloid synthesis through regulating the expression of key enzymes in MVA pathway.

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Abbreviations

TIA:

Terpenoid indole alkaloid

IPP:

Isopentenyl diphosphate

DMAPP:

Dimethylallyl diphosphate

MVA:

Mevalonate

MEP:

Methylerythritol phosphate

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Acknowlegements

The present investigation is financially supported by Scientific Research Project of Anhui Provincial Department of Education (KJ2015A005) and The Biology Key Subject Construction of Anhui, Collaborative Innovation Center of Agri-forestry Industry in Dabieshan Area, Anhui Agricultural University.

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    Authors and Affiliations

    1. School of Life Sciences, Anhui Agricultural University, Hefei, 230036, People’s Republic of China

      Yanfang Zhu, Cancan Meng, Liqin Zhu, Dahui Li, Qing Jin, Cheng Song, Yongping Cai, Honghong Fan & Yi Lin

    2. School of Life Sciences, Huaibei Normal University, Huaibei, 235000, People’s Republic of China

      Yanfang Zhu

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    1. Yanfang Zhu
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    Correspondence to Honghong Fan or Yi Lin.

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    Yanfang Zhu and Cancan Meng have contributed equally to the work.

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    Zhu, Y., Meng, C., Zhu, L. et al. Cloning and characterization of DoMYC2 from Dendrobium officinale . Plant Cell Tiss Organ Cult 129, 533–541 (2017). https://doi.org/10.1007/s11240-017-1198-3

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    • DOI: https://doi.org/10.1007/s11240-017-1198-3

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