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A third species of Nothoaspis Keirans & Clifford, 1975 (Acari: Argasidae): Nothoaspis setosus (Kohls, Clifford & Jones, 1969) n. comb

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Abstract

In South America, early descriptions of soft tick species were based on examination of the external morphology of the larval stages. In many cases, specimens were collected only once as it is the case of some bat-associated Ornithodoros spp. If we are to understand the systematic scenario of South American soft ticks, these species become axial questions to be re-studied from a morphological and molecular point of view. The objective of this study was to assess the taxonomic identity of soft tick larvae collected on bats inhabiting crevices of a large rock in the Rondônia State (RO), Brazilian Amazon. After a detailed morphological analysis using light microscopy, three large engorged larvae sharing the same phenotype were identified as Ornithodoros setosus Kohls, Clifford & Jones, 1969, a species collected in 1964 on bats in RO. Remarkably, maximum parsimony and Bayesian phylogenetic analyses based on tick 16S rRNA mitochondrial gene sequences obtained from two of these specimens showed that O. setosus indeed corresponds to a species of Nothoaspis Keirans & Clifford, 1975. Therefore, a new combination, Nothoaspis setosus (Kohls, Clifford & Jones, 1969), is herein proposed. While an elongated triangular dorsal plate with a curvy-notched posterior margin, and bulges in the lateral margins of basis capitulum correspond to common characters in larvae of the genus Nothoaspis, polymorphic traits are represented by minute cornua in the basis of the capitulum, the dentition of the hypostomal tip, triangular spurs on coxae I, and the number of dorsal and circumanal setae.

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Acknowledgements

We thank Simone Delgado Tojal and Ademir da Silva Quadros, for their valuable help during field work.

Funding

SML was funded by the “Fundação de Amparo à Pesquisa do Estado de São Paulo” (FAPESP), Grant #2018/02521-1. Laboratory work was supported by the “Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brasil” (CAPES), finance Code 001.

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Authors and Affiliations

  1. Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Orlando Marques de Paiva, 87, Cidade Universitária, São Paulo, SP, 05508-270, Brazil

    Sebastián Muñoz-Leal, Glauber M. B. Oliveira & Marcelo B. Labruna

  2. São Lucas Educacional, Rua Alexandre Guimarães, No. 1927 Bairro, Areal, Porto Velho, RO, Brazil

    Flavio A. Terassini & Luís Marcelo A. Camargo

  3. Mestrado em Medicina e Bem estar Animal, Universidade de Santo Amaro, São Paulo, SP, Brazil

    Arlei Marcili

  4. Instituto de Ciências Biomédicas 5, Universidade de São Paulo, Monte Negro, RO, Brazil

    Luís Marcelo A. Camargo

  5. Centro de Pesquisa em Medicina Tropical/SESAU, Porto Velho, RO, Brazil

    Luís Marcelo A. Camargo

  6. Instituto Nacional de Pesquisa e Tecnologia de Epidemiologia da Amazônia Ocidental (INCT/EpiAmo), Rio de Janeiro, RJ, Brazil

    Luís Marcelo A. Camargo

Authors
  1. Sebastián Muñoz-Leal
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  2. Flavio A. Terassini
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  3. Arlei Marcili
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  4. Glauber M. B. Oliveira
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  5. Luís Marcelo A. Camargo
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  6. Marcelo B. Labruna
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Corresponding author

Correspondence to Sebastián Muñoz-Leal.

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The authors declare that they have no conflict of interest.

Ethical approval

Animal captures, handling, and the collection of biological samples was approved by the “Instituto Chico Mendes da Conservação da Biodiversidade (ICMBio)” of the Brazilian government with the licenses 66376-1 and 31954 for collection of ticks and bats, respectively. All applicable international, national, and institutional guidelines for the care and use of animals were followed.

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Muñoz-Leal, S., Terassini, F.A., Marcili, A. et al. A third species of Nothoaspis Keirans & Clifford, 1975 (Acari: Argasidae): Nothoaspis setosus (Kohls, Clifford & Jones, 1969) n. comb. Syst Parasitol 96, 595–602 (2019). https://doi.org/10.1007/s11230-019-09873-9

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  • DOI: https://doi.org/10.1007/s11230-019-09873-9

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