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Russian Journal of Plant Physiology

, Volume 52, Issue 6, pp 758–764 | Cite as

Regulation of Water Permeability of Vacuolar Symplast

  • G. A. Velikanov
  • L. P. Belova
Article

Abstract

Effect of exogenous ABA and an inhibitor of energy metabolism NaN3 on water permeability of the desmotubules and tonoplast as the structural elements of vacuolar symplast ensuring water permeability of this transport system was investigated. The methodological approach based on the use of NMR with magnetic field pulse gradient is described in detail. It was shown that ABA affects water permeability of the vacuolar symplast in the root cells of maize (Zea mays L.) seedlings by temporary increase in water permeability of its membrane (tonoplast) and does not modify water permeability of desmotubules. At the same time, the effect of sodium azide is related to the disturbance of water permeability in the latter, and this evidence is corroborated by the additivity in the effects of the two above-mentioned agents on diffusion decay of spin echo produced by vacuolar symplast water molecules. ABA effect was detected only at high exogenous concentrations (10−4 M). The effect of ABA on water permeability of the tonoplast did not depend on or was weakly related to intracellular concentration of ATP, whereas the open state of desmotubules was ATP-dependent. Observations were made on the role of aquaporins in the ABA influence on tonoplast water permeability and the physiological role of high ABA concentrations.

Key words

Zea mays roots plasmodesmata desmotubules vacuolar symplast water permeability NMR 

Abbreviations

DD

diffusion decay of the echo

MFPG NMR

magnetic field pulse gradient NMR

SDC

self-diffusion coefficient

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Copyright information

© MAIK "Nauka/Interperiodica" 2005

Authors and Affiliations

  • G. A. Velikanov
    • 1
  • L. P. Belova
    • 1
  1. 1.Institute of Biochemistry and Biophysics, Kazan Research CenterRussian Academy of SciencesKazan, TatarstanRussia

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