Tissue-Specific Features of Pigment Biogenesis in Coleoptiles of Greening Etiolated Seedlings of Cereals
- 23 Downloads
Biogenesis of the pigment apparatus was studied in coleoptiles of postetiolated barley seedlings (Hordeum vulgare L.) and triticale (Triticale), differing in chlorophyll content, during growing in a “ light-darkness” regime with a 16-h photoperiod. Photoactive protochlorophyllide with a fluorescence maximum at 655 nm (Pchlide655), which accumulates in coleoptiles of etiolated seedlings, was converted in the light into a chlorophyll pigment with a fluorescence maximum at 690 nm (excitation at 440 nm, temperature −196°C). The spectral transition 690 nm → 675 nm forms was completed in darkness for 15 min illumination. There was almost no resynthesis of new portions of Pchlide655 in coleoptiles under darkness conditions, even after a 5–6-h darkness period after brief illumination of seedlings with flashes of white light. Chlorophyllide (Chlide) formed from Pchlide655 was not esterified and was destroyed both in the light (4 h, 1.0–1.5 klx) and darkness. In coleoptiles of greening etiolated seedlings, chlorophyll formation started only by 24 h of illumination. The instability of the chlorophyll pigment formed after etiolation indicates that plastids of coleoptiles do not contain the system of chlorophyll biosynthesis centers typical of leaves, which are bound to membranes and protect pigment from destruction.
Key wordsHordeum vulgare Triticale coleoptile leaf etiolation greening protochlorophyllide and its forms chlorophyllide
Unable to display preview. Download preview PDF.
- 1.Kabashnikova, L.F., Abramchik, L.M., Zenevich, L.A., Serdyuchenko, E.V., and Savchenko, G.E., Structural and Functional Peculiarities of the Pigment Apparatus of Cereal Coleoptiles, Mater. mezhd. nauch. konf. Molekulyarnye, membrannye i kletochnye osnovy funktsionirovaniya biosistem (Proc. Int. Sci. Conf. Molecular, Membrane, and Cellular Basics for Biosystem Functions), Minsk, 2004, part 1, pp. 57–59.Google Scholar
- 2.Klimovich, A.S., Chaika, M.T., Serdyuchenko, E.V., and Savchenko, G.E., Organ-Specific Regulation of Chlorophyll Accumulation in Coleoptiles of Green Barley (Hordeum vulgare L.) Seedlings, Fiziol. Rast. (Moscow), 1999, vol. 46, pp. 559–566 (Russ. J. Plant Physiol., Engl. Transl., pp. 482–489).Google Scholar
- 3.Savchenko, G.E., Abramchik, L.M., Serdyuchenko, E.V., and Chaika, M.T., Identification of Protochlorophyllide Oxidase in Barley Seedling Coleoptiles, Dokl. Natl. Akad. Nauk Belarusi, 1994, vol. 38, pp. 71–75.Google Scholar
- 4.Savchenko, G.E., Klyuchareva, E.A., and Kabashnikova, L.F., The System of Accumulation and Photoreduction of Protoclrorophyllide in Coleoptile from Etiolated Cereal Seedlings, Dokl. Natl. Akad. Nauk Belarusi, 2004, vol. 48, pp. 81–83.Google Scholar
- 5.Kaler, V.L. and Podchufarova, G.M., Correlation between the Content of Changed Protochlorophyllide and the Rate of Its Resynthesis in Etiolated Cereal Seedlings, Fotosintez i pitanie rastenii (Plant Photosynthesis and Nutrition), Minsk: Nauka i Tekhnika, 1969, pp. 27–31.Google Scholar
- 6.Domanskii, V.P., Samoilenko, A.G., and Lis, P.I., Automatic System Controlled by Micro-ECM, Izv. Akad. Nauk BSSR, Ser. Biol. Nauk, 1986, no. 1, pp. 100–102.Google Scholar
- 7.Shlyk, A.A., Averina, N.G., and Shalygo, N.V., Metabolism and Intermembrane Location of Magnesium-Protoporphyrin IX Monomethyl Ester in Centers of Chlorophyll Biosynthesis, Photochem. Photobiol., 1982, vol. 3, pp. 197–223.Google Scholar
- 8.Shlyk, A.A., Chaika, M.T., Fradkin, L.I., Rudoi, A.B., Averina, N.G., and Savchenko, G.E., Biogenesis of Photosynthetic Apparatus in Etiolated Leaves during Greening, Photochem. Photobiol., 1986, vol. 12, pp. 87–96.Google Scholar
- 9.Savchenko, G.E., Klyuchareva, E.A., Abramchik, L.M., Serdyuchenko, E.V., Minkov, I.N., and Chaika, M.T., Protochlorophyllide Oxidoreductase and Chlorophyll Biosynthesis in Greening Barley Seedlings, Fiziol. Rast. (Moscow), 1995, vol. 42, pp. 559–566 (Russ. J. Plant Physiol., Engl. Transl., pp. 492–499).Google Scholar
- 10.Rudiger, W., Chlorophyll Synthetase and Its Implication for Regulation of Chlorophyll Biosynthesis, Progress in Photosynthesis Research, Biggins, J., Ed., Dordrecht: Martinus Nijhoff, 1987, vol. 4, pp. 461–467.Google Scholar