Russian Journal of Plant Physiology

, Volume 52, Issue 2, pp 205–208 | Cite as

Antioxidant capacity of Brassica juncea plants exposed to elevated levels of copper

  • S. R. Devi
  • M. N. V. Prasad


Brassica juncea L. eight-day-old seedlings treated with various concentrations (50–200 µM) of copper for 48 h accumulated Cu more in the roots than in leaves. Accumulation of copper resulted in more active lipid peroxidation and depletion of glutathione (GSH) pools in both roots and shoots, which was attributed to copper-induced additional oxidative stress. Activities of ascorbate peroxidase and superoxide dismutase were higher in both roots and shoots while catalase activity increased in leaves but remained unchanged in roots in response to copper accumulation. Changes in lipid peroxidation, GSH content, and antioxidant enzyme activities suggest that oxidative damage may be involved in copper toxicity.

Key words

Brassica juncea antioxidants copper lipid peroxidation oxidative stress 



ascorbate peroxidase




reduced glutathione


oxidized glutathione




nitro blue tetrazolium


reactive oxygen species


superoxide dismutase


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Copyright information

© MAIK “Nauka/Interperiodica” 2005

Authors and Affiliations

  • S. R. Devi
    • 1
  • M. N. V. Prasad
    • 2
  1. 1.Department of BotanyIowa State UniversityIowaUSA
  2. 2.Department of Plant Sciences, School of Life SciencesUniversity of HyderabadHyderabadIndia

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