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Russian Journal of Plant Physiology

, Volume 52, Issue 1, pp 35–42 | Cite as

Changes in the content of individual lipid classes of a lichen Peltigera aphthosa during dehydration and subsequent rehydration

  • E. R. Kotlova
  • N. F. Sinyutina
Article

Abstract

A lichen Peltigera aphthosa (L.) Willd. was subjected to a short-term (7 days) or a long-term (180 and 540 days) dehydration followed by rehydration. Then the composition and content of lipids, as well as the rate of their metabolism (the rate of sodium 2-14C-acetate incorporation) were investigated. The long-term dehydration resulted in a dramatic decrease in the content (per dry wt) of major extrachloroplastic phospholipids, mainly phosphatidylcholines and phosphatidylethanolamines. The rehydration of lichen thalli after a short-term and long-term dehydration also resulted in an enhanced breakdown of these lipid molecules; however, it was accompanied by their rather intense in vivo synthesis, which was decreased after long-term dehydration. In contrast to phospholipids, the betaine lipids, diacylglyceroltrimethylhomoserines (DGTSs), were involved in metabolic processes to a far lesser extent. In the course of rehydration, their content was virtually unchanged and decreased only after 540 days of dehydration. The rate of incorporation of sodium 2-14C-acetate into the DGTS molecules was moderate and did not change even after long-term dehydration. Glycolipids were characterized by a fair tolerance to hydrolytic processes and by an increase in the rate of their synthesis after 540 days of the lichen dehydration. Responses of neutral lipids to dehydration turned out to be different. The long-term dehydration (for 540 days) was accompanied by a decrease in the contents of free sterols and sterol esters, whereas the contents of di- and triacylglycerols remained unchanged. Rehydration resulted in a decrease in diacylglycerol and sterol ester contents. All neutral lipids were characterized by a dramatic decrease in the rate of de novo synthesis after long-term dehydration. It was suggested that the tolerance of lichen to long-term dehydration was appreciably determined by the tolerance of its phycobiont, in this case, a green alga Coccomyxa sp.; the bulk of its lipids was characterized by a minimum rate of breakdown and, at the same time, by a stable synthesis.

Key words

Peltigera aphthosa lichens lipids dehydration rehydration 

Abbreviations

DAG

diacylglycerol

DGDG

digalactosyldiacylglycerol

DGTS

diacylglyceroltrimethylhomoserine

FA

fatty acid

MGDG

monogalactosyldiacylglycerol

PC

phosphatidylcholine

PE

phosphatidylethanolamine

PG

phosphatidylglycerol

PI

phosphatidylinositol

SQDG

sulfoquinovosyldiacylglycerol

TAG

triacylglycerol

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Copyright information

© MAIK “Nauka/Interperiodica” 2005

Authors and Affiliations

  • E. R. Kotlova
    • 1
  • N. F. Sinyutina
    • 2
  1. 1.Komarov Botanical InstituteRussian Academy of SciencesSt. PetersburgRussia
  2. 2.Biological Research InstituteSt. PetersburgRussia

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