Skip to main content
SpringerLink
Log in

The ecdysoneless 1 Gene Regulates Metabolism of the Juvenile Hormone and Dopamine in Drosophila melanogaster

  • General Genetics
  • Published:
Russian Journal of Genetics Aims and scope Submit manuscript

Abstract

The dopamine (DA) content and the level of juvenile hormone (JH) degradation were studied in females of the wild-type Canton S strain and the ecdysoneless 1 (ecd 1) mutant, which does not produce ecdysone at a restrictive temperature (29°C). Exposure at the restrictive temperature considerably increased the JH-hydrolyzing activity and the DA content in five-day ecd 1 females compared with flies of both strains growing at 19°C and Canton S females exposed at 29°C. In one-day ecd 1 females, the level of JH degradation also increased at the restrictive temperature, but the DA content was low. The effect of ecdysone deficiency on the stress reaction in Drosophila melanogaster females was studied using changes in DA content and JH degradation as the reaction indicators. The ecd 1 mutation did not prevent the initiation of the stress reaction in females exposed at the restrictive temperature, but changed its intensity (stress reactivity). The interaction of 20-hydroxyecdysone with JH and DA in regulating Drosophila reproduction under normal conditions and in stress is discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  1. Garen, A., Kauvar, L., and Lepesant, J.-A., Roles of Ecdysone in Drosophila Development, Proc. Natl. Acad. Sci. USA, 1977, vol. 74, pp. 5099–5103.

    CAS  PubMed  Google Scholar 

  2. Redfern, C.P.F. and Bownes, M., Pleiotropic Effects of the ecdysoneless-1 Mutation of Drosophila melanogaster, Mol. Gen. Genet., 1983, vol. 189, pp. 432–440.

    Article  CAS  Google Scholar 

  3. Henrich, V.C., Tucker, R.L., Maroni, G., and Gilbert, L.I., The ecdysoneless (ecd1 ts) Mutation Disrupts Ecdysteroid Synthesis Autonomously in the Ring Gland of Drosophila melanogaster, Dev. Biol., 1987, vol. 120, pp. 50–55.

    Article  PubMed  CAS  Google Scholar 

  4. Dai, J.D., Henrich, V.C., and Gilbert, L.I., An Ultrastructural Analysis of the ecdysoneless (l(3)ecd1 ts) Ring Gland during the Third Larval Instar of Drosophila melanogaster, Cell Tissue Res., 1991, vol. 265, pp. 435–445.

    Article  PubMed  CAS  Google Scholar 

  5. Warren, J.T., Bachmann, J.S., Dai, J.D., and Gilbert, L.I., Differential Incorporation of Cholesterol and Cholesterol Derivatives into Ecdysteroids by the Larval Ring Glands and Adult Ovaries of Drosophila melanogaster: A Putative Explanation for the l(3)ecd1 1 Mutation, Insect Biochem. Mol. Biol., 1996, vol. 26, pp. 931–943.

    PubMed  CAS  Google Scholar 

  6. Audit-Lamour, C. and Busson, D., Oogenesis Defects in the ecd-1 Mutant of Drosophila melanogaster, Deficient in Ecdysteroid at High Temperature, J. Insect Physiol., 1981, vol. 27, pp. 829–837.

    Article  CAS  Google Scholar 

  7. Koeppe, J.K., Fuchs, M., Chen, T.T., et al., The Role of Juvenile Hormone in Reproduction, Comprehensive Insect Physiology, Biochemistry and Pharmacology, Kerkut, G.A. and Gilbert, L.I., Eds., New York: Pergamon, 1985, vol. 8, pp. 165–203.

    Google Scholar 

  8. Kelly, T.J., Endocrinology of Vitellogenesis in Drosophila melanogaster, Perspectives in Comparative Endocrynology, Davey, K.J., Tobe, S.S., and Peters, R.P., Eds., Ottawa: National Research Council of Canada, 1994, pp. 282–290.

    Google Scholar 

  9. Bownes, M., Dubendorfer, A., and Smith, T., Ecdysteroids in Adult Males and Females of Drosophila melanogaster, J. Insect Physiol., 1984, vol. 30, pp. 823–830.

    Article  CAS  Google Scholar 

  10. Richard, D.S., Watkins, N.L., Serafin, R.B., and Gilbert, L.I., Ecdysteroids Regulate Yolk Protein Uptake by Drosophila melanogaster Oocytes, J. Insect Physiol., 1998, vol. 44, pp. 637–644.

    Article  PubMed  CAS  Google Scholar 

  11. Richard, D.S., Jones, J.M., Barbarito, M.R., et al., Vitellogenesis in Diapausing and Mutant Drosophila melanogaster: Further Evidence for the Relative Roles of Ecdysteroids and Juvenile Hormones, J. Insect Physiol., 2001, vol. 47, pp. 905–913.

    CAS  Google Scholar 

  12. Soller, M., Bownes, M., and Kubli, E., Control of Oocyte Maturation in Sexually Mature Drosophila Females, Dev. Biol., 1999, vol. 208, pp. 337–351.

    Article  PubMed  CAS  Google Scholar 

  13. Gruntenko, N.E., Bownes, M., Terashima, J., et al., Heat Stress Affects Oogenesis Differently in Wild-Type Drosophila virilis and a Mutant with Altered Juvenile Hormone and 20-Hydroxyecdysone Levels, Insect Mol. Biol., 2003, vol. 12, pp. 393–404.

    PubMed  CAS  Google Scholar 

  14. Rauschenbach, I.Yu., Gruntenko, N.E., Bownes, M., et al., The Role of Juvenile Hormone in the Control of Reproductive Function in Drosophila virilis under Nutritional Stress, J. Insect Physiol., 2004, vol. 50, pp. 323–330.

    Google Scholar 

  15. Rauschenbach, I.Yu., Gruntenko, N.E., Chentsova, N.A., et al., Regulation of Reproductive Function in Drosophila Female due to Hormonal Interaction under Stress Is Genetically Determined, Rus. J. Genet., 2001, vol. 37, no.9, pp. 1041–1047.

    Article  CAS  Google Scholar 

  16. Rauschenbach, I.Yu., Gruntenko, N.E., Karpova, E.K., et al., 20-Hydroxyecdysone Interacts with Juvenile Hormone and Dopamine in Controlling Fertility Drosophila virilis, Dokl. RAN, 2005, vol. 400, no.6, pp. 847–849.

    Google Scholar 

  17. Gruntenko, N.E. and Rauschenbach, I.Yu., Adaptive Value of Genes Controlling the Level of Biogenic Amines in Drosophila, Rus. J. Genet., 2004, vol. 40, no.7, pp. 703–709.

    Article  CAS  Google Scholar 

  18. Rauschenbach, I.Yu., Serova, L.I., Timochina, I.S., et al., Analysis of Differences in Dopamine Content between Two Lines of Drosophila virilis in Response to Heat Stress, J. Insect Physiol., 1993, vol. 39, no.9, pp. 761–767.

    Article  CAS  Google Scholar 

  19. Rauschenbach, I.Y., Khlebodarova, T.M., Chentsova, N.A., et al., Metabolism of the Juvenile Hormone in Drosophila Adults under Normal Conditions and Heat Stress, J. Insect Physiol., 1995, vol. 41, pp. 179–189.

    CAS  Google Scholar 

  20. Rauschenbach, I.Yu., Gruntenko, N.E., Khlebodarova, T.M., et al., The Role of the Degradation System of the Juvenile Hormone in the Reproduction of Drosophila under Stress, J. Insect Physiol., 1996, vol. 42, pp. 735–742.

    Article  CAS  Google Scholar 

  21. Gruntenko, N.E., Khlebodarova, T.M., Sukhanova, M.Jh., et al., Prolonged Negative Selection of Drosophila melanogaster for a Character of Adaptive Significance Disturbs Stress Reactivity, Insect Biochem. Mol. Biol., 1999, vol. 29, pp. 445–452.

    PubMed  CAS  Google Scholar 

  22. Rauschenbach, I.Yu., Gruntenko, N.E., Vasenkova, I.A., et al., On Some Regularities of Genetic Control of Drosophila Stress Reaction, Rus. J. Genet., 2001, vol. 37, no.1, pp. 64–70.

    Article  CAS  Google Scholar 

  23. Hirashima, A., Sukhanova, M.Jh., Kuwano, E., and Rauschenbach, I.Yu., Alteration of Biogenic Amines in Drosophila virilis under Stress, Dros. Inf. Serv., 1999, vol. 82, pp. 30–31.

    Google Scholar 

  24. Gruntenko, N.E., Chentsova, N.A., Bogomolova, E.V., et al., The Effect of Mutations Altering Biogenic Amine Metabolism in Drosophila on Viability and the Response to Environmental Stresses, Arch. Ins. Biochem. Physiol., 2004, vol. 55, pp. 55–67.

    Article  CAS  Google Scholar 

  25. Hammock, B.D. and Sparks, T.C., A Rapid Assay for Insect Juvenile Hormone Esterase Activity, Anal. Biochem., 1977, vol. 82, pp. 573–579.

    Article  PubMed  CAS  Google Scholar 

  26. Maikel, P., Cox, R.H., Saillant, J., and Miller, F.P., A Method for the Determination of Serotonin and Noradrenaline in Discrete Areas of Rat Brain, Int. J. Neuropharmacol., 1968, vol. 7, pp. 275–278.

    Google Scholar 

  27. Gruntenko, N.E., Monastirioti, M., and Rauschenbach, I.Yu., Biogenic Amines Control Metabolism of the Juvenile Hormone in Drosophila melanogaster Adults, Dokl. Ros. Akad. Nauk, 2001, vol. 376, pp. 427–429.

    CAS  Google Scholar 

  28. Postlethwait, J.H. and Handler, A.M., Nonvitellogenic Female-Sterile Mutants and the Regulation of Vitellogenesis in Drosophila melanogaster, Dev. Biol., 1978, vol. 67, pp. 202–213.

    Article  PubMed  CAS  Google Scholar 

  29. Postlethwait, J.H. and Parker, J., Regulation of Vitellogenesis in Drosophila, Molecular Biology of Invertebrate Development, O'Connor, J.D., Ed., New York: Liss, 1987, pp. 29–42.

    Google Scholar 

  30. Postlethwait, J.H. and Shirk, P.D., Genetic and Endocrine Regulation of Vitellogenesis in Drosophila, Am. Zool., 1981, vol. 21, pp. 687–700.

    CAS  Google Scholar 

  31. Jowett, T. and Postlethwait, J.H., Hormonal Regulation of Synthesis of Yolk Proteins and a Larval Serum Protein (LSP2) in Drosophila, Nature, 1981, vol. 292, pp. 633–635.

    Article  PubMed  CAS  ISI  Google Scholar 

  32. Bownes, M., Expression of Genes Coding for Vitellogenin (Yolk Protein), Ann. Rev. Entomol., 1986, vol. 31, pp. 507–531.

    Article  CAS  Google Scholar 

  33. Richard, D.S., Gilbert, M., Crum, B., et al., Yolk Protein Endocytosis in Drosophia melanogaster: Immunofluorescent Localization of Clathrin, Adaptin and Yolk Protein Receptor, J. Insect Physiol., 2001, vol. 47, pp. 715–723.

    PubMed  CAS  Google Scholar 

  34. Altaratz, M., Applebaum, Sh.W., Richard, D.S., et al., Regulation of Juvenile Hormone Synthesis in Wild-Type and apterous Mutant Drosophila, Mol. Cell. Endocrinol., 1991, vol. 81, pp. 205–216.

    Article  PubMed  CAS  Google Scholar 

  35. Gruntenko, N.E., Chentsova, N.A., Andreenkova, E.V., et al., Stress Reaction in a Juvenile Hormone-Deficient Drosophila melanogaster Mutant apterous 56f, Insect Mol. Biol., 2003, vol. 12, pp. 353–363.

    PubMed  CAS  Google Scholar 

  36. Thompson, C.S., Yagi, K.J., Chen, Z.F., and Tobe, S.S., The Effects of Octopamine on Juvenile Hormone Biosynthesis, Electrophysiology, and cAMP Content of the corpora allata of the Cockroach Diploptera punctata, J. Comp. Physiol. B, 1990, vol. 160, pp. 241–249.

    Article  PubMed  CAS  Google Scholar 

  37. Pastor, D., Piulachs, M.D., Cassier, P., et al., In Vivo and in Vitro Study of the Action of Dopamine on Oocyte Growth and Juvenile Hormone Production in Blattella germanica, CR Acad. Sci. III, 1991, vol. 313, pp. 207–212.

    CAS  Google Scholar 

  38. Woodring, J. and Hoffman, K.H., The Effects of Octopamine, Dopamine and Serotonin on Juvenile Hormone Synthesis, in Vitro, in the Cricket, Gryllus bimaculatus, J. Insect Physiol., 1994, vol. 40, pp. 797–802.

    CAS  Google Scholar 

  39. Granger, N.A., Sturgis, S.L., Ebersohl, R., et al., Dopaminergic Control of corpora allata Activity in the Larval Tobacco Hornworm, Manduca sexta, Arch. Insect Biochem. Physiol., 1996, vol. 32, pp. 449–466.

    Article  PubMed  CAS  Google Scholar 

  40. Hirashima, A., Suetsugu, E., Hirokado, S., et al., Effect of Octopamine on the Activity of Juvenile-Hormone Esterase in the Silkworm Bombyx mori and the Red Flour Beetle Tribolium freemani, Gen. Comp. Endocrinol., 1999, vol. 116, pp. 373–381.

    Article  PubMed  CAS  Google Scholar 

  41. Rauschenbach, I.Yu., Adon'eva, N.V., Gruntenko, N.E., et al., Synthesis and Degradation of the Juvenile Hormone Are under a Common Control in Drosophila, Dokl. Ros. Akad. Nauk, 2002, vol. 386, pp. 136–138.

    Google Scholar 

  42. Rauschenbach, I.Yu., Gruntenko, N.E., Chentsova, N.A., et al., A Feedback in Regulation of the Juvenile Hormone Titer by Biogenic Amines in Drosophilidae, Dokl. Ros. Akad. Nauk, 2004, vol. 397, pp. 427–429.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Cytology and Genetics, Russian Academy of Sciences, Novosibirsk, 630090, Russia

    E. K. Karpova, N. E. Gruntenko & I. Yu. Rauschenbach

Authors
  1. E. K. Karpova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. N. E. Gruntenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. I. Yu. Rauschenbach
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

__________

Translated from Genetika, Vol. 41, No. 11, 2005, pp. 1480–1486.

Original Russian Text Copyright © 2005 by Karpova, Gruntenko, Rauschenbach.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Karpova, E.K., Gruntenko, N.E. & Rauschenbach, I.Y. The ecdysoneless 1 Gene Regulates Metabolism of the Juvenile Hormone and Dopamine in Drosophila melanogaster . Russ J Genet 41, 1217–1222 (2005). https://doi.org/10.1007/s11177-005-0222-6

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11177-005-0222-6

Keywords

Search

Navigation