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Russian Journal of Genetics

, Volume 41, Issue 9, pp 1008–1013 | Cite as

Mitogenic and Mutagenic Effects of Ionized Air on Allium fistulosum L.

  • V. A. Trofimov
  • T. A. Pyansina
Plant Genetics
  • 26 Downloads

Abstract

In the apical meristem of Allium fistulosum, the relationship between peroxide lipid oxidation, antioxidant activity, proliferative processes, the yield of chromosomal aberrations and duration the exposure to ionized air was studied. Under the influence of air oxygen ions, superoxide dismutase and catalase activities increased, proliferative processes were stimulated, and shifts occurred in the process of lipid peroxidation in cells of A. fistulosum. When these cells were treated with air oxygen for 40 min, hydrogen peroxide and iron sulfate (II) enhanced oxygen biostimulating effect via stimulation of antioxidant enzyme activity and inhibition of lipid peroxidation. Under these conditions, cell proliferation was intensified and the yield of chromosomal aberrations was reduced in A. fistulosum rootlets. When the time of seed treatment with ionized air was increased to 80 min, lipid peroxidation was activated, antioxidant enzyme activity was inhibited, and the yield of chromosomal aberration increased in seedlings. It was concluded that the biostimulating activity of ionized air was mediated by active oxygen species generated in the cell. The accumulation of TBA(thiobarbituric acid)-reactive products was shown to be related to a decrease in antioxidant enzyme activity and an increase in the yield of chromosomal aberrations. It is emphasized that the mutagenic effect of ionized air is associated with generating conditions that support Fenton reaction and OH-radical formation in the cell.

Keywords

Lipid Peroxidation Catalase Superoxide Dismutase Lipid Oxidation Catalase Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Gus'kov, E.P., Varduni, T.V., Shkurat, T.P., et al., Free Radical Processes and the Chromosome Aberration Frequency in Tree Leaves As Tests for Genotoxicity of the Urban Environment, Ekologiya, 2000, no. 4, pp. 270–275.Google Scholar
  2. 2.
    Imlay, J.A. and Linn, S., DNA Damage and Oxygen Radical Toxity, Science, 1988, vol. 240, pp. 1302–1309.PubMedGoogle Scholar
  3. 3.
    Breen, A.P. and Murphy, J.A., Reaction of Oxyl Radicals with DNA, Free Radical Biol. Med., 1995, vol. 18, pp. 1033–1077.CrossRefGoogle Scholar
  4. 4.
    Bilski, P., Burkhart, J.G., and Chignell, C.F., Photochemical Characterization of Water Samples from Minnesota and Vermont Sites with Malformed Frogs: Potential Influence of Photosensitization by Singlet Molecular Oxygen (1O2) and Free Radicals on Aquatic Toxicity, Aquat. Toxicol., 2003, vol. 65, pp. 229–241.CrossRefPubMedGoogle Scholar
  5. 5.
    Brown, K. and Fridovich, I., DNA Strand Session by Enzymatic Ally Generated Oxygen Radicals, Arch. Biochem. Biophys., 1981, vol. 206, pp. 414–419.CrossRefPubMedGoogle Scholar
  6. 6.
    Benov, L. and Beema, A., Superoxide Dependence of the Short Chain Sugars-Induced Mutagenesis, Free Radical Biol. Med., 2002, vol. 34, no.4, pp. 429–433.CrossRefGoogle Scholar
  7. 7.
    Burbon, R.N., Free Radicals and Cell Proliferation, New Comp. Biochem., 1994, vol. 28, pp. 155–185.Google Scholar
  8. 8.
    Trofimov, V.A., Ashirov, R.Z., and Vlasov, A.P., Biokhimicheskie metody issledovaniya lipidov v klinike (Biochemical Methods of Lipid Studies in Clinics), Saransk: Mordovsk. Gos. Univ., 2001, pp. 62–64.Google Scholar
  9. 9.
    Korolyuk, M.A., Catalase Activity Assay, Lab. Delo, 1988, no. 1, pp. 18–19.Google Scholar
  10. 10.
    Burlakova, E.B., Role of Antioxidants in Physicochemical Processes Regulating Cell Proliferation, Byull. Mosk. O-va Ispyt. Prir., Otd. Biofiz., 1968, vol. 28, pp. 15–23.Google Scholar
  11. 11.
    Shkurat, T.P., Dynamics of Lipid Oxidation and Chromosome Rearrangements in Various Mouse Tissues after Repetitive Hyperbaric Exposure, Fiziol. Zh., 1993, no. 1, pp. 91–98.Google Scholar
  12. 12.
    Saprin, A.N. and Kalinina, E.V., Oxidative Stress and Its Role in Mechanisms of Apoptosis and Pathological Processes, Usp. Biol. Khim., 1999, vol. 39, pp. 289–326.Google Scholar

Copyright information

© MAIK "Nauka/Interperiodica" 2005

Authors and Affiliations

  • V. A. Trofimov
    • 1
  • T. A. Pyansina
    • 1
  1. 1.Department of GeneticsOgarev Mordovian State UniversitySaranskRussia

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