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Russian Journal of Genetics

, Volume 41, Issue 6, pp 593–601 | Cite as

The dds20+ Gene Controls a Novel Rad51Sp-Dependent Pathway of Recombinational Repair in Schizosaccharomyces pombe

  • A. F. Salakhova
  • G. V. Savchenko
  • F. K. Khasanov
  • O. V. Chepurnaya
  • V. G. Korolev
  • V. I. Bashkirov
Molecular Genetics

Abstract

Repair of DNA double-strand break (DSB) is an evolutionary conserved Rad51-mediated mechanism. In yeasts, Rad51 paralogs, Saccharomyces cerevisiae Rad55-Rad57 and Schizosaccharomyces pombe Rhp55-Rhp57 are mediators of the nucleoprotein Rad51 filament formation. As shown in this work, a novel Rad51Sp-dependent pathway of DSB repair acts in S. pombe parallel to the pathway mediated by Rad51 paralogs. A new gene dds20+ that controls this pathway was identified. The overexpression of dds20+ partially suppresses defects of mutant rhp55Δ in DNA repair. Cells of dds20Δ manifest hypersensitivity to a variety of genotoxins. Epistatic analysis revealed that dds20+ is a gene of the recombinational repair group. The role of Dds20 in repair of spontaneous damages occurring in the process of replication and mating-type switching remains unclear. The results obtained suggest that Dds20 has functions beyond the mitotic S phase. The Dds20 protein physically interacts with Rhp51(Rad51Sp). Dds20 is assumed to operate at early recombinational stages and to play a specific role in the Rad51 protein filament assembly differing from that of Rad51 paralogs.

Keywords

Saccharomyces Cerevisiae Gene Control Filament Formation Repair Group Recombinational Repair 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© MAIK “Nauka/Interperiodica” 2005

Authors and Affiliations

  • A. F. Salakhova
    • 1
  • G. V. Savchenko
    • 1
  • F. K. Khasanov
    • 1
  • O. V. Chepurnaya
    • 2
  • V. G. Korolev
    • 2
  • V. I. Bashkirov
    • 1
  1. 1.Institute of Gene BiologyRussian Academy of SciencesMoscowRussia
  2. 2.Konstantinov Institute of Nuclear PhysicsRussian Academy of SciencesGatchina, Leningrad oblastRussia

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