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The dds20 + Gene Controls a Novel Rad51Sp-Dependent Pathway of Recombinational Repair in Schizosaccharomyces pombe

  • Molecular Genetics
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Abstract

Repair of DNA double-strand break (DSB) is an evolutionary conserved Rad51-mediated mechanism. In yeasts, Rad51 paralogs, Saccharomyces cerevisiae Rad55-Rad57 and Schizosaccharomyces pombe Rhp55-Rhp57 are mediators of the nucleoprotein Rad51 filament formation. As shown in this work, a novel Rad51Sp-dependent pathway of DSB repair acts in S. pombe parallel to the pathway mediated by Rad51 paralogs. A new gene dds20 + that controls this pathway was identified. The overexpression of dds20 + partially suppresses defects of mutant rhp55Δ in DNA repair. Cells of dds20Δ manifest hypersensitivity to a variety of genotoxins. Epistatic analysis revealed that dds20 + is a gene of the recombinational repair group. The role of Dds20 in repair of spontaneous damages occurring in the process of replication and mating-type switching remains unclear. The results obtained suggest that Dds20 has functions beyond the mitotic S phase. The Dds20 protein physically interacts with Rhp51(Rad51Sp). Dds20 is assumed to operate at early recombinational stages and to play a specific role in the Rad51 protein filament assembly differing from that of Rad51 paralogs.

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Authors and Affiliations

  1. Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334, Russia

    A. F. Salakhova, G. V. Savchenko, F. K. Khasanov & V. I. Bashkirov

  2. Konstantinov Institute of Nuclear Physics, Russian Academy of Sciences, Gatchina, Leningrad oblast, 188300, Russia

    O. V. Chepurnaya & V. G. Korolev

Authors
  1. A. F. Salakhova
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  2. G. V. Savchenko
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  3. F. K. Khasanov
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  4. O. V. Chepurnaya
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  5. V. G. Korolev
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  6. V. I. Bashkirov
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__________

Translated from Genetika, Vol. 41, No. 6, 2005, pp. 736–745.

Original Russian Text Copyright © 2005 by Salakhova, Savchenko, Khasanov, Chepurnaya, Korolev, Bashkirov.

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Salakhova, A.F., Savchenko, G.V., Khasanov, F.K. et al. The dds20 + Gene Controls a Novel Rad51Sp-Dependent Pathway of Recombinational Repair in Schizosaccharomyces pombe . Russ J Genet 41, 593–601 (2005). https://doi.org/10.1007/s11177-005-0132-7

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  • DOI: https://doi.org/10.1007/s11177-005-0132-7

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