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Russian Journal of Genetics

, Volume 40, Issue 12, pp 1426–1429 | Cite as

Distribution of transposons Tn5044 and Tn5070 with noncanonical mer operons in environmental bacterial populations

  • Zh. M. Gorlenko
  • E. S. Kalyaeva
  • I. A. Bass
  • M. A. Petrova
  • S. Z. Mindlin
Short Communications
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Abstract

The distribution of noncanonical mercury resistance transposons, Tn5044 and Tn5070 , was examined. A characteristic feature of Tn5044 is temperature sensitivity of its mercury operon and the presence in the mer operon of the gene homologous to RNA polymerase ∑ subunit. Structural organization of mercury operon Tn5070 , containing minimum gene set (merRTPA), differs from mer operons of both Gram-negative and Gram-positive bacteria. None of more than two thousand environmental bacterial strains displaying mercury resistance and isolated from the samples selected from different geographical regions hybridized to Tn5044- and Tn5070-specific probes. A concept on the existence of cosmopolite, endemic, and rare transposons in environmental bacterial populations was formulated.

Keywords

Mercury Bacterial Strain Characteristic Feature Geographical Region Structural Organization 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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REFERENCES

  1. 1.
    Osborn, A.M., Bruce, K.D., Strike, P., Ritchie, D.A. 1997Distribution, Diversity and Evolution of the Bacterial Mercury Resistance (mer) OperonFEMS Microbiol. Rev.19239262Google Scholar
  2. 2.
    Hobman, J.L., Brown, N.L. 1997Bacterial Mercury Resistance GenesSigel, H.Sigel, A. eds. Metal Ions in Biological SystemsMarcel DekkerNew York527567Google Scholar
  3. 3.
    Barkay, T., Miller, S.M., Summers, A.O. 2003Bacterial Mercury Resistance from Atoms to EcosystemsFEMS Microbiol. Rev.27355384Google Scholar
  4. 4.
    Pearson, A.J., Bruce, K.D., Osborn, A.M.,  et al. 1996Distribution of Class II Transposase and Resolvase Genes in Soil Bacteria and Their Association with mer GenesAppl. Environ. Microbiol.6229612965Google Scholar
  5. 5.
    Liebert, C.A., Wireman, J., Smith, T., Summers, A.O. 1997Phylogeny of Mercury Resistance (mer) Operons of Gram-Negative Bacteria Isolated from the Fecal Flora of PrimatesAppl. Environ. Microbiol.6310661076Google Scholar
  6. 6.
    Yurieva, O., Kholodii, G., Minakhin, L.,  et al. 1997Intercontinental Spread of Promiscuous Mercury Resistance Operons in Environmental BacteriaMol. Microbiol.24321329Google Scholar
  7. 7.
    Bogdanova, E.S., Bass, I.A., Minakhin, L.S.,  et al. 1998Horizontal Spread of mer Operons among Gram-Positive Bacteria in Natural EnvironmentsMicrobiology144609620Google Scholar
  8. 8.
    Liebert, C.A., Hall, R.M., Summers, A.O. 1999Transposon Tn21, Flagship of the Floating GenomeMicrobiol. Mol. Biol. Rev.63507522Google Scholar
  9. 9.
    Minakhina, S., Kholodii, G., Mindlin, S.Z.,  et al. 1999Tn5053 Family Transposons Are res Sites Hunters Sensing Plasmidal res Sites Occupied by Cognate ResolvaseMol. Microbiol.3310591068Google Scholar
  10. 10.
    Bogdanova, E., Minakhin, L., Bass, I.,  et al. 2001Class II Broad-Spectrum Mercury Resistance Transposons in Gram-Positive Bacteria from Natural EnvironmentsRes. Microbiol.152503514Google Scholar
  11. 11.
    Mindlin, S., Kholodii, G., Gorlenko, Zh.,  et al. 2001Mercury Resistance Transposons of Gram-Negative Environmental Bacteria and Their ClassificationRes. Microbiol.152811822Google Scholar
  12. 12.
    Mindlin, S.Z., Bass, I.A., Bogdanova, E.S.,  et al. 2002Horizontal Transfer of Mercury Resistance Genes in Environmental Bacterial PopulationsMol. Biol.36216227(Moscow)Google Scholar
  13. 13.
    Kholodii, G.Ya., Mindlin, S.Z., Bass, I.A.,  et al. 1995Four Genes, Two Ends, and a res Region Are Involved in Transposition of Tn5053: A Paradigm for a Novel Family of Transposons Carrying Either a mer Operon or an IntegronMol. Microbiol.1711891200Google Scholar
  14. 14.
    Kholodii, G.Ya., Yurieva, O.V., Gorlenko, Zh.M.,  et al. 1997Tn5041: A Chimeric Mercury Resistance Transposon Closely Related to a Toluene Degradative Transposon Tn4651Microbiology14325492556Google Scholar
  15. 15.
    Kholodii, G., Gorlenko, Zh., Mindlin, S.,  et al. 2002Tn5041-Like Transposons: Molecular Diversity, Evolutionary Relationships and Distribution of Distinct Variants in Environmental BacteriaMicrobiology14835693582Google Scholar
  16. 16.
    Kholodii, G., Yurieva, O., Mindlin, S.,  et al. 2000Tn5044, a Novel Tn3 Family Transposon Coding for Temperature-Sensitive Mercury ResistanceRes. Microbiol.151291302Google Scholar
  17. 17.
    Kholodii, G., Bogdanova, E. 2002Tn5044-Conferred Mercury Resistance Depends on Temperature: The Complexity of the Character of ThermosensitivityGenetics115233241Google Scholar
  18. 18.
    Helmann, J.D., Wang, Y., Mahler, I., Walsh, C.T. 1989Homologous Metalloregulatory Proteins from Both Gram-Positive and Gram-Negative Bacteria Control Transcription of Mercury Resistance OperonsJ. Bacteriol.171222229Google Scholar
  19. 19.
    Brown, N.L., Stoyanov, J.V., Kidd, S.P., Hobman, J.L. 2003The MerR Family of Transcriptional RegulatorsFEMS Microbiol. Rev.27145163Google Scholar
  20. 20.
    Osborn, A.M., Bruce, K.D., Rithie, D.A., Strike, P. 1996The Mercury Resistance Operon of the IncJ Plasmid pMERPH Exhibits Structural and Regulatory Divergence from Other Gram-Negative mer OperonsMicrobiology142337345Google Scholar
  21. 21.
    Iohara, K., Iiyama, R., Nakamura, K.,  et al. 2001The mer Operon of a Mercury-Resistant Pseudoalteromonas haloplanktis Strain Isolated from Minamata Bay, JapanAppl. Microbiol. Biotechnol.56736741Google Scholar

Copyright information

© MAIK “Nauka/Interperiodica” 2004

Authors and Affiliations

  • Zh. M. Gorlenko
    • 1
  • E. S. Kalyaeva
    • 1
  • I. A. Bass
    • 1
  • M. A. Petrova
    • 1
  • S. Z. Mindlin
    • 1
  1. 1.Institute of Molecular GeneticsRussian Academy of SciencesMoscowRussia

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