Russian Journal of Bioorganic Chemistry

, Volume 31, Issue 6, pp 543–548 | Cite as

L-Glutamic Acid Modulates the Cytotoxic Effect of Tumor Necrosis Factor on the HL-60 Cell Line

  • N. V. Gibanova
  • T. V. Rakitina
  • S. S. Zhokhov
  • N. M. Pustoshilova
  • V. M. Lipkin
  • I. A. Kostanyan


L-Glutamic acid was shown to increase the stability of cells of the HL-60 line of human promyelocyte leukemia to the cytotoxic action of tumor necrosis factor alpha (TNF-α) due to the inhibition of apoptotic and NF-κB-activating cascades induced by this cytokine. At the same time, L-glutamic acid increases the TNF-α-mediated differentiating signal and the accompanying enhancement of the phosphatidylinositol-specific phospholipase C activity. Therefore, it is a promising agent for the reduction of total toxicity and inflammatory processes during treatment with TNF-α.

Key words

apoptosis cellular differentiation L-glutamic acid HL-60 cells NF-κB transcription factor phosphatidylinositol-specific phospholipase C tumor necrosis factor alpha 



Fas-Associated Death Domain Protein


fluorescein isothiocyanate


inositol mono-, di-, and triphosphates


phosphate-buffered saline


phosphatidylinositol-specific phospholipase C


phosphatidylinositol 4,5-bisphosphate




TNF-α-Receptor Interacting Protein


Tumor Necrosis Factor alpha


receptor of TNFα


TNF-R1-Associated Death Domain Protein


TNF-Receptor-Associated Factor


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Copyright information

© MAIK "Nauka/Interperiodica" 2005

Authors and Affiliations

  • N. V. Gibanova
    • 1
  • T. V. Rakitina
    • 1
  • S. S. Zhokhov
    • 1
  • N. M. Pustoshilova
    • 2
  • V. M. Lipkin
    • 1
  • I. A. Kostanyan
    • 1
  1. 1.Shemyakin-Ovchinnikov Institute of Bioorganic ChemistryRussian Academy of SciencesMoscowRussia
  2. 2.Research and Technology Institute of Biologically Active SubstancesVector State Scientific Center for Virology and BiotechnologyBerdsk, Novosibirsk oblastRussia

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