Russian Journal of Bioorganic Chemistry

, Volume 31, Issue 4, pp 401–404 | Cite as

A New Method for Studying in vitro Lipid Transport: The 45-kDa Protein from Rat Olfactory Epithelium Is a Specific Carrier of Phosphatidylinositol 3,4,5-Triphosphate

  • V. V. Radchenko
  • T. M. Shuvaeva
  • E. V. Il’nitskaya
  • V. E. Tret’yakov
  • V. M. Lipkin
Letters to the Editor


A new method for studying lipid-protein interactions in vitro is developed. It enables the study of the transporting activity of a protein toward a lipid ligand, including the case of an unknown lipid type. The method can be considered as a variant of partition three-phase chromatography with two stationary (donor and acceptor) phases and one mobile phase. The protein under study is dissolved in an aqueous mobile phase and induces a specific delivery of a lipid to the acceptor lipid layer. The transported lipid is identified in Folch lipid extracts from the acceptor layer and aqueous phase. The secretory protein with M 45 kDa from the rat olfactory epithelium is shown to be a carrier of phosphatidylinositol 3,4,5-triphosphate. Our approach opens up new possibilities in the study of lipid-protein interactions in vitro and has a number of advantages over the methods now used for these purposes.

Key words

lipid-protein interactions lipid-transporting proteins phosphatidylinositol 3,4,5-triphosphate secretory protein p45 



bovine serum albumin


lipid-transporting protein


polyacrylamide gel


polyethylene glycol




phosphatidylinositol 3,4,5-triphosphate


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  1. 1.
    Gennis, R.B., Biomembranes: Molecular Structure and Function, New York: Academic, 1989. Translated under the title Biomembrany: Molekulyarnaya struktura i funktsii, Moscow: Mir, 1997.Google Scholar
  2. 2.
    Deak, M., Csamayor, A., Currie, R.A., Downes, C.P., and Alessia, D.R., FEBS Lett., 1999, vol. 451, pp. 220–226.CrossRefPubMedGoogle Scholar
  3. 3.
    Ishii, M., Fujita, S., Yamada, M., Hosaka, Y., and Kurachi, Y., Biochem. J., 2005, vol. 385, pp. 65–73.CrossRefPubMedGoogle Scholar
  4. 4.
    Komatsu, H., Bouma, B., Wirtz, K.W., Taraschi, T.F., and Janes, N., Biochem. J., 2000, vol. 348, pp. 667–673.CrossRefPubMedGoogle Scholar
  5. 5.
    Kasper, A.M. and Helmkamp, Jr., G.M, Biochemistry, 1981, vol. 20, pp. 146–151.CrossRefPubMedGoogle Scholar
  6. 6.
    Demel, R.A., Wirtz, K.W.A., Kamp, H.H., van Kessel, G.W.S.M., and van Deenen, L.L.M., Nature New Biology, 1973, vol. 246, pp. 102–105.PubMedGoogle Scholar
  7. 7.
    Merkulova, M.I., Andreeva, S.G., Shuvaeva, T.M., Novoselov, S.V., Peshenko, I.V., Bystrova, M.F., Novoselov, V.I., Fesenko, E.E., and Lipkin, V.M., FEBS Lett., 1999, vol. 450, pp. 126–130.CrossRefPubMedGoogle Scholar
  8. 8.
    Merkulova, M.M., Radchenko, V.V., Il’nitskaya, E.V., Shuvaeva, T.M., and Lipkin, V.M., Bioorg. Khim., 2005, vol. 31, pp. 280–287.PubMedGoogle Scholar
  9. 9.
    Folch, J., Less, M., and Sloane, G., J. Biol. Chem., 1957, vol. 226, pp. 497–509.PubMedGoogle Scholar

Copyright information

© MAIK “Nauka/Interperiodica” 2005

Authors and Affiliations

  • V. V. Radchenko
    • 1
  • T. M. Shuvaeva
    • 1
  • E. V. Il’nitskaya
    • 1
  • V. E. Tret’yakov
    • 2
  • V. M. Lipkin
    • 1
  1. 1.Shemyakin-Ovchinnikov Institute of Bioorganic ChemistryRussian Academy of SciencesMoscowRussia
  2. 2.Research Institute of Physicochemical MedicineMinistry of Health and Social Development of the Russian FederationMoscowRussia

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