Abstract
A new method for studying lipid-protein interactions in vitro is developed. It enables the study of the transporting activity of a protein toward a lipid ligand, including the case of an unknown lipid type. The method can be considered as a variant of partition three-phase chromatography with two stationary (donor and acceptor) phases and one mobile phase. The protein under study is dissolved in an aqueous mobile phase and induces a specific delivery of a lipid to the acceptor lipid layer. The transported lipid is identified in Folch lipid extracts from the acceptor layer and aqueous phase. The secretory protein with M 45 kDa from the rat olfactory epithelium is shown to be a carrier of phosphatidylinositol 3,4,5-triphosphate. Our approach opens up new possibilities in the study of lipid-protein interactions in vitro and has a number of advantages over the methods now used for these purposes.
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Abbreviations
- BSA:
-
bovine serum albumin
- LTP:
-
lipid-transporting protein
- PAAG:
-
polyacrylamide gel
- PEG:
-
polyethylene glycol
- PtdCho:
-
phosphatidylcholine
- PtdIns(3,4,5)P 3 :
-
phosphatidylinositol 3,4,5-triphosphate
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Translated from Bioorganicheskaya Khimiya, Vol. 31, No. 4, 2005, pp. 445–448.
Original Russian Text Copyright ¢ 2005 by Radchenko, Shuvaeva, Il’nitskaya, Tret’yakov, Lipkin.
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Radchenko, V.V., Shuvaeva, T.M., Il’nitskaya, E.V. et al. A New Method for Studying in vitro Lipid Transport: The 45-kDa Protein from Rat Olfactory Epithelium Is a Specific Carrier of Phosphatidylinositol 3,4,5-Triphosphate. Russ J Bioorg Chem 31, 401–404 (2005). https://doi.org/10.1007/s11171-005-0056-y
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DOI: https://doi.org/10.1007/s11171-005-0056-y