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Russian Journal of Bioorganic Chemistry

, Volume 31, Issue 4, pp 401–404 | Cite as

A New Method for Studying in vitro Lipid Transport: The 45-kDa Protein from Rat Olfactory Epithelium Is a Specific Carrier of Phosphatidylinositol 3,4,5-Triphosphate

  • V. V. Radchenko
  • T. M. Shuvaeva
  • E. V. Il’nitskaya
  • V. E. Tret’yakov
  • V. M. Lipkin
Letters to the Editor

Abstract

A new method for studying lipid-protein interactions in vitro is developed. It enables the study of the transporting activity of a protein toward a lipid ligand, including the case of an unknown lipid type. The method can be considered as a variant of partition three-phase chromatography with two stationary (donor and acceptor) phases and one mobile phase. The protein under study is dissolved in an aqueous mobile phase and induces a specific delivery of a lipid to the acceptor lipid layer. The transported lipid is identified in Folch lipid extracts from the acceptor layer and aqueous phase. The secretory protein with M 45 kDa from the rat olfactory epithelium is shown to be a carrier of phosphatidylinositol 3,4,5-triphosphate. Our approach opens up new possibilities in the study of lipid-protein interactions in vitro and has a number of advantages over the methods now used for these purposes.

Key words

lipid-protein interactions lipid-transporting proteins phosphatidylinositol 3,4,5-triphosphate secretory protein p45 

Abbreviations

BSA

bovine serum albumin

LTP

lipid-transporting protein

PAAG

polyacrylamide gel

PEG

polyethylene glycol

PtdCho

phosphatidylcholine

PtdIns(3,4,5)P3

phosphatidylinositol 3,4,5-triphosphate

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Copyright information

© MAIK “Nauka/Interperiodica” 2005

Authors and Affiliations

  • V. V. Radchenko
    • 1
  • T. M. Shuvaeva
    • 1
  • E. V. Il’nitskaya
    • 1
  • V. E. Tret’yakov
    • 2
  • V. M. Lipkin
    • 1
  1. 1.Shemyakin-Ovchinnikov Institute of Bioorganic ChemistryRussian Academy of SciencesMoscowRussia
  2. 2.Research Institute of Physicochemical MedicineMinistry of Health and Social Development of the Russian FederationMoscowRussia

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