A New Method for Studying in vitro Lipid Transport: The 45-kDa Protein from Rat Olfactory Epithelium Is a Specific Carrier of Phosphatidylinositol 3,4,5-Triphosphate
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A new method for studying lipid-protein interactions in vitro is developed. It enables the study of the transporting activity of a protein toward a lipid ligand, including the case of an unknown lipid type. The method can be considered as a variant of partition three-phase chromatography with two stationary (donor and acceptor) phases and one mobile phase. The protein under study is dissolved in an aqueous mobile phase and induces a specific delivery of a lipid to the acceptor lipid layer. The transported lipid is identified in Folch lipid extracts from the acceptor layer and aqueous phase. The secretory protein with M 45 kDa from the rat olfactory epithelium is shown to be a carrier of phosphatidylinositol 3,4,5-triphosphate. Our approach opens up new possibilities in the study of lipid-protein interactions in vitro and has a number of advantages over the methods now used for these purposes.
Key wordslipid-protein interactions lipid-transporting proteins phosphatidylinositol 3,4,5-triphosphate secretory protein p45
bovine serum albumin
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