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Russian Journal of Bioorganic Chemistry

, Volume 31, Issue 1, pp 99–103 | Cite as

An express morphine assay in aqueous samples by immunochromatography using monoclonal antibodies labeled with colloidal gold

  • I. A. Lyubavina
  • A. A. Zinchenko
  • M. I. Lapenkov
  • T. L. Nikolaeva
Article

Abstract

A simple and accessible express method was developed for the detection of morphine in aqueous samples using competitive immunochromatography. A complex of colloidal gold with monoclonal antibodies to morphine was used as the detection agent. The detection limit for morphine in aqueous samples was 10 ng/ml, and the analysis time was 5 min.

Key words

immunochromatography monoclonal antibodies to morphine, complex with colloidal gold morphine 

Abbreviations

Ab

monoclonal antibodies to morphine

Ab-HRP

a conjugate of the antibodies to morphine with horseradish peroxidase

Ab-gold

a complex of monoclonal antibodies to morphine with colloidal gold

EIA

enzyme immunoassay

HRP

horseradish peroxidase

M-OVA

polyvalent conjugate of ovalbumin with morphine

PBS

phosphate-buffered saline, pH 7.4

PBST

PBS containing 0.05% Tween-20

PBS/OVA

PBS containing ovalbumin (2 mg/ml)

PBST/OVA

PBST containing ovalbumin (2 mg/ml)

RIA

radioisotope assay

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Copyright information

© MAIK “Nauka/Interperiodica” 2005

Authors and Affiliations

  • I. A. Lyubavina
    • 1
  • A. A. Zinchenko
    • 1
  • M. I. Lapenkov
    • 2
  • T. L. Nikolaeva
    • 2
  1. 1.Shemyakin-Ovchinnikov Institute of Bioorganic ChemistryRussian Academy of SciencesMoscowRussia
  2. 2.National Hematology Research CenterRussian Academy of Medical SciencesMoscowRussia

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