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An express morphine assay in aqueous samples by immunochromatography using monoclonal antibodies labeled with colloidal gold

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Abstract

A simple and accessible express method was developed for the detection of morphine in aqueous samples using competitive immunochromatography. A complex of colloidal gold with monoclonal antibodies to morphine was used as the detection agent. The detection limit for morphine in aqueous samples was 10 ng/ml, and the analysis time was 5 min.

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Abbreviations

Ab:

monoclonal antibodies to morphine

Ab-HRP:

a conjugate of the antibodies to morphine with horseradish peroxidase

Ab-gold:

a complex of monoclonal antibodies to morphine with colloidal gold

EIA:

enzyme immunoassay

HRP:

horseradish peroxidase

M-OVA:

polyvalent conjugate of ovalbumin with morphine

PBS:

phosphate-buffered saline, pH 7.4

PBST:

PBS containing 0.05% Tween-20

PBS/OVA:

PBS containing ovalbumin (2 mg/ml)

PBST/OVA:

PBST containing ovalbumin (2 mg/ml)

RIA:

radioisotope assay

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Authors and Affiliations

  1. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117997, Moscow, Russia

    I. A. Lyubavina & A. A. Zinchenko

  2. National Hematology Research Center, Russian Academy of Medical Sciences, Novozykovskii proezd 4a, 125167, Moscow, Russia

    M. I. Lapenkov & T. L. Nikolaeva

Authors
  1. I. A. Lyubavina
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  2. A. A. Zinchenko
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  3. M. I. Lapenkov
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  4. T. L. Nikolaeva
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Corresponding author

Correspondence to I. A. Lyubavina.

Additional information

Translated from Bioorganicheskaya Khimiya, Vol. 31, No. 1, 2005, pp. 108–112.

Original Russian Text Copyright © 2005 by Lyubavina, Zinchenko, Lapenkov, Nikolaeva.

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Lyubavina, I.A., Zinchenko, A.A., Lapenkov, M.I. et al. An express morphine assay in aqueous samples by immunochromatography using monoclonal antibodies labeled with colloidal gold. Russ J Bioorg Chem 31, 99–103 (2005). https://doi.org/10.1007/s11171-005-0014-8

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  • DOI: https://doi.org/10.1007/s11171-005-0014-8

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