Research on Chemical Intermediates

, Volume 42, Issue 11, pp 7701–7711 | Cite as

The time-series evaluation of biohydrogen production by photosynthetic bacteria under fluctuating illumination pattern

  • Naoki Ikenaga
  • Saki Okamura
  • Ken Shibata
  • Nobuyuki Tanaka
  • Jun Miyake


In recent years, world climate change and global warming have been big issues. One of the solutions is to use renewable energies; however, renewable energies have an intermittent nature. In the case of photovoltaic arrays, the intermittency is mainly caused by fluctuating irradiation from sunlight due to clouds. In this study, biohydrogen production from photosynthetic bacteria was focused for the use of fluctuating sunlight irradiation. Previous researches have revealed some characteristics of biohydrogen production, and these results enable one to expect that photosynthetic bacteria have fluctuating light tolerance of biohydrogen production, in which the bacteria are able to produce biohydrogen continuously under fluctuating light irradiation. There have been quite a few studies to evaluate time-course changes of biohydrogen production under fluctuating irradiation, and therefore time-course evaluations have been performed. A 10-min light/dark illumination pattern was set for the fluctuating irradiation and the magnitude of the fluctuation was used to evaluate the fluctuation of the hydrogen production rate and irradiation light. The results indicated that the fluctuation was 0.22 times smaller through the photosynthetic bacteria. The results of this study indicate that photosynthetic bacteria have fluctuating light tolerance. Biohydrogen production, having fluctuating light tolerance, would be useful for realistic use of sunlight energy as renewable energy.


Biohydrogen Photofermentation Photosynthetic bacteria Time-change evaluations 



This study was supported by the Strategic International Research Cooperative Program “Microbial Consortia Engineering and System Optimization for Gas Bio-fuel Production from Biomass” from Japan Science Technology Agency, Japan.


  1. 1.
    D.B. Levin, L. Pitt, M. Love, Biohydrogen production: prospects and limitations to practical application. Int. J. Hydrogen Energy 29(2), 173–185 (2004)CrossRefGoogle Scholar
  2. 2.
    W.F. Pickard, D. AbBott, Addressing the intermittency challenge: massive energy storage in a sustainable future. Proc. IEEE 100, 317–321 (2012)CrossRefGoogle Scholar
  3. 3.
    L.A.S. Ribeiro, O.R. Saavedra, S.L. Lima, J.G. de Matos, G. Bonan, Making isolated renewable energy systems more reliable. Renew Energy 45, 221–231 (2012)CrossRefGoogle Scholar
  4. 4.
    A.E. Curtright, J. Apt, The character of power output from utility-scale photovoltaic systems. Prog. Photovolt. Res. Appl. 16, 241–247 (2008)CrossRefGoogle Scholar
  5. 5.
    P.H. Tsaslides, A. Thanailakis, Loss-of-load probability and related parameters in optimum computer-aided design of stand-alone photovoltaic systems. Solar Cells 18, 115–127 (1986)CrossRefGoogle Scholar
  6. 6.
    J. Lagorse, M.G. Simoes, A. Miraoui, P. Costerg, Energy cost analysis of a solar-hydrogen hybrid energy system for stand-alone applications. Int. J. Hydrogen Energy 33, 2871–2879 (2008)CrossRefGoogle Scholar
  7. 7.
    A. Murata, K. Otani, An analysis of time-dependent spatial distribution of output power from very many PV power systems installed on a nation-wide scale in Japan. Sol. Energy Mater. Sol. Cell 47, 197–202 (1997)CrossRefGoogle Scholar
  8. 8.
    H. Gaffron, J. Rubin, Fermentative and photochemical production of hydrogen in algae. J. Gen. Physiol. 26, 219–240 (1942)CrossRefGoogle Scholar
  9. 9.
    M.S. Kim, D.H. Kim, H.N. Son, L.N. Ten, J.K. Lee, Enhancing photo-fermentative hydrogen production by Rhodobacter sphaeroides KD131 and its PHB synthase deleted-mutant from acetate and butyrate. Int. J. Hydrogen Energy 36, 13964–13971 (2011)CrossRefGoogle Scholar
  10. 10.
    Y. Öztürk, M. Yücel, F. Daldal, S. Mandacı, U. Gündüz, L. Türker, I. Eroglu, Hydrogen production by using Rhodobacter capsulatus mutants with genetically modified electron transfer chains. Int. J. Hydrogen Energy 31, 1545–1552 (2006)CrossRefGoogle Scholar
  11. 11.
    T. Kondo, M. Arakawa, T. Hirai, T. Wakayama, M. Hara, J. Miyake, Enhancement of hydrogen production by a photosynthetic bacterium mutant with reduced pigment. J. Biosci. Bioeng. 93, 145–150 (2002)CrossRefGoogle Scholar
  12. 12.
    T. Matsunaga, T. Hatano, A. Yamada, M. Matsumoto, Microaerobic hydrogen production by photosynthetic bacteria in a double-phase photobioreactor. Biotechnol. Bioeng. 68, 647–651 (2000)CrossRefGoogle Scholar
  13. 13.
    J. Miyake, X.Y. Mao, S. Kawamura, Photoproduction of hydrogen from glucose by a co-culture of a photosynthetic bacterium and clostridium butyricum. J. Ferment. Technol. 62, 531–535 (1984)Google Scholar
  14. 14.
    D. Das, T.N. Veziroglu, Hydrogen production by biological processes: a survey of literature. Int. J. Hydrogen Energy 26, 13–28 (2001)CrossRefGoogle Scholar
  15. 15.
    J. Miyake, T. Wakayama, J. Schnackenberg, T. Arai, Y. Asada, Simulation of the daily sunlight illumination pattern for bacterial photo-hydrogen production. J. Biosci. Bioeng. 88, 659–663 (1999)CrossRefGoogle Scholar
  16. 16.
    T. Wakayama, J. Miyake, Light shade bands for the improvement of solar hydrogen production efficiency by Rhodobacter sphaeroides RV. Int. J. Hydrogen Energy 27, 1495–1500 (2002)CrossRefGoogle Scholar
  17. 17.
    T. Wakayama, E. Nakada, Y. Asada, J. Miyake, Effect of light/dark cycle on bacterial hydrogen production by Rhodobacter sphaeroides RV from hour to second range. Appl. Biochem. Biotechnol. 84–86, 431–440 (2000)CrossRefGoogle Scholar
  18. 18.
    H. Koku, I. Eroglu, U. Gunduz, M. Yucel, L. Turker, Kinetics of biological hydrogen production by the photosynthetic bacterium Rhodobacter sphaeroides O.U. 001. Int. J. Hydrogen Energy 28, 381–388 (2003)CrossRefGoogle Scholar
  19. 19.
    Y. Asada, M. Tokumoto, Y. Aihara, M. Oku, K. Ishimi, T. Wakayama, J. Miyake, M. Tomiyama, H. Kohno, Hydrogen production by co-cultures of Lactobacillus and a photosynthetic bacterium, Rhodobacter sphaeroides RV. Int. J. Hydrogen Energy 31, 1509–1513 (2006)CrossRefGoogle Scholar
  20. 20.
    N. Kawasaki, K. Kitamura, H. Sugihara, S. Nishikawa, K. Kurokawa, An evaluation of area-dependency equalization of fluctuation characteristics from distributed PV systems. Proc. Renew. Energy 2006, 471–474 (2006)Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Naoki Ikenaga
    • 1
  • Saki Okamura
    • 2
  • Ken Shibata
    • 1
  • Nobuyuki Tanaka
    • 1
  • Jun Miyake
    • 1
    • 2
  1. 1.Department of Mechanical Science and Bioengineering, Graduate School of Engineering ScienceOsaka UniversityToyonakaJapan
  2. 2.Graduate School of Frontier BiosciencesOsaka UniversityToyonakaJapan

Personalised recommendations