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Research on Chemical Intermediates

, Volume 35, Issue 5, pp 607–613 | Cite as

Effect of ultraviolet irradiation on photostability of C-phycocyanin in a silica matrix

  • Ye Li
  • Hui Yang
  • Fang Ming Cao
Article

Abstract

Application of protein encapsulation silica materials requires biomolecules to maintain stabilization on potentially adverse industrial conditions. The C-phycocyanin (C-PC) is chosen as a probe to monitor influence of the protein encapsulation process and to study protein and matrix interaction and stability of protein in silica matrix. Both C-PCs in solution and in silicate matrix irradiated by ultraviolet rays can result in photodestruction, whereas the protein in the silica is less affected. The measured photodamage rate constant of C-PC in silica is 25 times slower than that of C-PC in buffer solution. However, lifetimes of C-phycocyanin in phosphate buffer and silica matrix are identical. These studies suggest that entrapment of C-PCs into silica matrixes not only can keep their biological activity and obviously also improve their photostability.

Keywords

C-phycocyanin Photostability Silica Conformation 

Notes

Acknowledgments

This project was supported by the National Natural Science Foundation of China (Grant No. 20873005), Beijing Natural Science Foundation (Grant No. 2083028), the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry and 422 Funds from University of Science and Technology Beijing

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Copyright information

© Springer Science+Business Media BV 2009

Authors and Affiliations

  1. 1.Department of Chemistry, School of Applied ScienceUniversity of Science and Technology BeijingBeijingPeoples Republic of China

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