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Differentiation and evolutionary relationships in Erythrinus erythrinus (Characiformes, Erythrinidae): comparative chromosome mapping of repetitive sequences

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Abstract

Erythrinus erythrinus presents extensive karyotypic diversity, with four karyomorphs (A–D) differing in the number of chromosomes, karyotype structure or sex chromosomes systems. Karyomorph A has 2n = 54 chromosomes in males and females without heteromorphic sex chromosomes, while karyomorph C has 2n = 52 chromosomes in females and 2n = 51 chromosomes in males, due a X1X1X2X2/X1X2Y sex chromosome system. Three allopatric populations of the karyomorph A and one population of the karyomorph C were now in deep investigated by molecular cytogenetic analyses, using repetitive DNAs as probes. The results reinforced the relatedness among populations of the karyomorph A, despite their large geographic distribution. Karyomorph C, however, showed a remarkably difference in the genomic constitution, especially concerning the amount and distribution of the 5S rDNA and Rex3 sequences on chromosomes. In addition, although karyomorphs C and D share several features, exclusive chromosomal markers show the derivative evolutionary pathway between them. Thus, besides the classical chromosomal rearrangements, the repetitive DNAs were useful tools to reveal the biodiversity, relatedness and differentiation of this fish group. The chromosomal set strongly corroborates that E. erythrinus corresponds to a species complex instead of a single biological entity.

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Acknowledgments

This work was supported by the Brazilian agencies FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo), CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico), CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior), FAPEMAT (Fundação de Amparo à Pesquisa do Estado de Mato Grosso).

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Authors and Affiliations

  1. Departamento de Genética e Evolução, Universidade Federal de São Carlos (UFSCar), Rodovia Washington Luiz Km. 235, C.P. 676, São Carlos, SP, 13565-905, Brazil

    Nícolas Fernandes Martins, Luiz Antonio Carlos Bertollo & Marcelo de Bello Cioffi

  2. Departamento de Ciências Biológicas, Universidade do Estado de Mato Grosso, Campus de Tangará da Serra, Tangará da Serra, MT, 78300-000, Brazil

    Waldo Pinheiro Troy

  3. Laboratório de Genética Animal, Instituto Nacional de Pesquisas da Amazônia, C.P. 478, Manaus, AM, 69011-970, Brazil

    Eliana Feldberg & Francisco Carlos de Souza Valentin

Authors
  1. Nícolas Fernandes Martins
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  2. Luiz Antonio Carlos Bertollo
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  3. Waldo Pinheiro Troy
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  4. Eliana Feldberg
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  5. Francisco Carlos de Souza Valentin
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  6. Marcelo de Bello Cioffi
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Correspondence to Marcelo de Bello Cioffi.

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Martins, N.F., Bertollo, L.A.C., Troy, W.P. et al. Differentiation and evolutionary relationships in Erythrinus erythrinus (Characiformes, Erythrinidae): comparative chromosome mapping of repetitive sequences. Rev Fish Biol Fisheries 23, 261–269 (2013). https://doi.org/10.1007/s11160-012-9292-4

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  • DOI: https://doi.org/10.1007/s11160-012-9292-4

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