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Reviews in Fish Biology and Fisheries

, Volume 23, Issue 2, pp 261–269 | Cite as

Differentiation and evolutionary relationships in Erythrinus erythrinus (Characiformes, Erythrinidae): comparative chromosome mapping of repetitive sequences

  • Nícolas Fernandes Martins
  • Luiz Antonio Carlos Bertollo
  • Waldo Pinheiro Troy
  • Eliana Feldberg
  • Francisco Carlos de Souza Valentin
  • Marcelo de Bello Cioffi
Research Paper

Abstract

Erythrinus erythrinus presents extensive karyotypic diversity, with four karyomorphs (A–D) differing in the number of chromosomes, karyotype structure or sex chromosomes systems. Karyomorph A has 2n = 54 chromosomes in males and females without heteromorphic sex chromosomes, while karyomorph C has 2n = 52 chromosomes in females and 2n = 51 chromosomes in males, due a X1X1X2X2/X1X2Y sex chromosome system. Three allopatric populations of the karyomorph A and one population of the karyomorph C were now in deep investigated by molecular cytogenetic analyses, using repetitive DNAs as probes. The results reinforced the relatedness among populations of the karyomorph A, despite their large geographic distribution. Karyomorph C, however, showed a remarkably difference in the genomic constitution, especially concerning the amount and distribution of the 5S rDNA and Rex3 sequences on chromosomes. In addition, although karyomorphs C and D share several features, exclusive chromosomal markers show the derivative evolutionary pathway between them. Thus, besides the classical chromosomal rearrangements, the repetitive DNAs were useful tools to reveal the biodiversity, relatedness and differentiation of this fish group. The chromosomal set strongly corroborates that E. erythrinus corresponds to a species complex instead of a single biological entity.

Keywords

Fish Karyomorphs Karyotype evolution Repetitive DNAs Chromosomal markers 

Notes

Acknowledgments

This work was supported by the Brazilian agencies FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo), CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico), CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior), FAPEMAT (Fundação de Amparo à Pesquisa do Estado de Mato Grosso).

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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Nícolas Fernandes Martins
    • 1
  • Luiz Antonio Carlos Bertollo
    • 1
  • Waldo Pinheiro Troy
    • 2
  • Eliana Feldberg
    • 3
  • Francisco Carlos de Souza Valentin
    • 3
  • Marcelo de Bello Cioffi
    • 1
  1. 1.Departamento de Genética e EvoluçãoUniversidade Federal de São Carlos (UFSCar)São CarlosBrazil
  2. 2.Departamento de Ciências BiológicasUniversidade do Estado de Mato GrossoTangará da SerraBrazil
  3. 3.Laboratório de Genética AnimalInstituto Nacional de Pesquisas da AmazôniaManausBrazil

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