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Reviews in Fish Biology and Fisheries

, Volume 19, Issue 2, pp 167–176 | Cite as

The artificial incubation of crayfish eggs: review and report from an experimental study concerning the effects of offspring origin (maternal or artificial incubation) on the survival and growth of juvenile signal crayfish (Pacifastacus leniusculus, Astacidae)

  • Rocío González
  • Jesus D. Celada
  • Vanesa García
  • Álvaro González
  • José M. Carral
  • María Sáez-Royuela
Research Paper

Abstract

The development of artificial incubation techniques in astacid crayfish has attracted attention from scientists in many countries ever since the nineteenth century. It is only in the last few years that these techniques, along with studies on egg storage and transport, have provided reliable options for improving the reproductive phase in farming. The juveniles produced need to be reared until they reach a sufficient size both for restocking and for growing purposes. In view of the current level of knowledge of rearing juvenile astacids, two 80-day experiments were carried out under controlled conditions to compare the survival and growth of Stage 2 juvenile signal crayfish (Pacifastacus leniusculus) from two origins: maternal or artificial incubation. In the first experiment, three treatments were tested: juveniles from artificially incubated eggs with formaldehyde treatments, juveniles from maternal incubation and a mixture from both origins (50% each). Survival rates ranged from 87.8% to 93.3% with no significant differences among treatments. Crayfish from artificial incubation grew significantly faster (11.47 mm carapace length (CL), 373.80 mg weight) than crayfish from maternal incubation. In the second experiment, a bifactorial design included four treatments: the crayfish was derived from artificial or from maternal incubation and was fed once a day or twice a day. Final survival rates ranged from 68.89% to 77.78%, with no significant differences among treatments. Crayfish from artificial incubation grew significantly faster than crayfish from maternal incubation. The highest CL (14.54 mm) and weight (780.13 mg) were reached by the juveniles from artificial incubation that were fed once a day. No significant differences were found between the two feeding frequencies. Results showed that artificial incubation with formaldehyde treatments had no harmful effects and made it feasible to get a better performance from the juveniles obtained.

Keywords

Astacid crayfish Pacifastacus leniusculus Artificial incubation Juvenile rearing Offspring origin 

Notes

Acknowledgements

Funding of this study was the Plan Nacional de I + D+i, Ministerio de Educación y Ciencia, Spain, Research Project AGL2005-01127. We thank the financing of a grant for Programa Nacional de Formación de Profesorado Universitario, Ministerio de Educación y Ciencia, Spain, reference AP2005-4860. We should also like to thank the Quiñón S.A. crayfish farm for their collaboration.

References

  1. Ackefors H, Gydemo R, Westin L (1989) Growth and survival of juvenile crayfish, Astacus astacus, in relation to food and density. In: de Pauw N, Jaspers E, Ackefors H, Wilkins N (eds) Aquaculture—a biotechnology in progress. European Aquaculture Society, Bredene, pp 365–373Google Scholar
  2. Ackefors H, Castell JD, Boston LD, Räty P, Svensson M (1992) Standard experimental diets for crustacean nutrition research. II. Growth and survival of juvenile crayfish Astacus astacus (Linné) fed diets containing various amounts of protein, carbohydrate and lipid. Aquaculture 104:341–356. doi: 10.1016/0044-8486(92)90215-7 CrossRefGoogle Scholar
  3. Ackefors H, Gydemo R, Keyser P (1995) Growth and moulting in confined juvenile noble crayfish Astacus astacus (L.) (Decapoda: Astacidae). Freshw Crayfish 10:396–409Google Scholar
  4. Andrews EA (1904) Breeding habits of crayfish. Am Nat 38:165–206. doi: 10.1086/278387 CrossRefGoogle Scholar
  5. Arrignon J (1981) L’écrevisse et son élevage. Gauthier-Villars, ParisGoogle Scholar
  6. Blake M, Nyström P, Hart P (1994) The effect of weed cover on juvenile signal crayfish (Pacifastacus leniusculus Dana) exposed to adult crayfish and non-predatory fish. Ann Zool Fenn 31:297–306Google Scholar
  7. Brodsky SJ (1962) L′élevage artificiel des écrevisses de rivière - Une méthode perfectionnée de reproduction de leurs réserves. Vopr. Ecologii i Biosenologii 4Google Scholar
  8. Brodsky SJ (1984) Sur l’élevage en Union Soviétique des écrevisses de rivière (Astacidae) par la méthode industrielle et sur les perspectives de cette méthode. Piscic Fr 78:5–9Google Scholar
  9. Carral JM, Celada JD, Gaudioso VR, Temiño C, Fernández R (1988) Artificial incubation improvement of crayfish eggs (Pacifastacus leniusculus Dana) under low temperatures during embryonic development. Fresw Crayfish 7:239–250Google Scholar
  10. Carral JM, Celada JD, González J, Gaudioso VR, Fernández R, Lopez-Baissón C (1992) Artificial incubation of crayfish eggs (Pacifastacus leniusculus Dana) from early stages of embryonic development. Aquaculture 104:261–269. doi: 10.1016/0044-8486(92)90208-3 CrossRefGoogle Scholar
  11. Carral JM, Sáez-Royuela M, Celada JD, Pérez JR, Melendre PM, Aguilera A (2003) Advantages of artificial reproduction techniques for white-clawed crayfish (Austropotamobius pallipes Lereboullet). Bull Fr Peche Piscicult 370–371:181–184. doi: 10.1051/kmae:2003013 CrossRefGoogle Scholar
  12. Carral JM, Pérez JR, Celada JD, Sáez-Royuela M, Melendre PM, Aguilera A (2004) Effects of dead egg removal frequency on stage 2 juvenile production in artificial incubation of Austropotamobius pallipes Lereboullet. Bull Fr Peche Piscicult 372–373:425–430. doi: 10.1051/kmae:2004015 CrossRefGoogle Scholar
  13. Celada JD, Gaudioso VR, Paz P, Fernández R (1985) Identification et chronologie des phases de développement des œufs de l’écrevisse (Pacifastacus leniusculus Dana) par observation directe. Piscic Fr 82:5–8Google Scholar
  14. Celada JD, Paz P, Gaudioso VR, Fernández R (1987) Embryonic development of the freshwater crayfish (Pacifastacus leniusculus Dana): a scanning electron microscopic study. Anat Rec 219:304–310. doi: 10.1002/ar.1092190311 PubMedCrossRefGoogle Scholar
  15. Celada JD, Carral JM, Gaudioso VR, Temiño C, Fernández R (1989) Response of juvenile freshwater crayfish (Pacifastacus lenisculus Dana) to several fresh and artificially compounded diets. Aquaculture 76:67–78. doi: 10.1016/0044-8486(89)90252-4 CrossRefGoogle Scholar
  16. Celada JD, Carral JM, González J (1991) A study on the identification and chronology of the embryonic stages of the freshwater crayfish Austreopotamobius pallipes (Lereboullet, 1858). Crustaceana 61(3):225–232. doi: 10.1163/156854091X00119 CrossRefGoogle Scholar
  17. Celada JD, Carral JM, Gaudioso VR, González J, Lopez-Baissón C, Fernández R (1993) Survival and growth of juvenile freshwater crayfish Pacifastacus leniusculus Dana fed two raw diets and two commercial formulates feeds. J World Aquac Soc 24(1):108–111. doi: 10.1111/j.1749-7345.1993.tb00157.x CrossRefGoogle Scholar
  18. Celada JD, Carral JM, Sáez-Royuela M, Gaudioso VR, Muñoz MC, Pérez JR (1994) Investigación sobre cangrejos de río en la Universidad de León. Fac Vet 38:55–70Google Scholar
  19. Celada JD, González J, Carral JM, Fernández R, Pérez JR, Sáez-Royuela M (2000) Storage and transport of embryonated eggs of the signal crayfish, Pacifastacus leniusculus. N Am J Aquac 62:308–310. doi :10.1577/1548-8454(2000)062<0308:SATOEE>2.0.CO;2CrossRefGoogle Scholar
  20. Celada JD, Carral JM, Pérez JR, Sáez-Royuela M, Muñoz C (2001) Successful storage and transport of eggs of the white-clawed crayfish (Austropotamobius pallipes Lereboullet). Aquac Int 9:269–276. doi: 10.1023/A:1015337211733 CrossRefGoogle Scholar
  21. Celada JD, Carral JM, Sáez-Royuela M, Melendre PM, Aguilera A (2004) Effects of different antifungal treatments on artificial incubation of the astacid crayfish (Pacifastacus leniusculus Dana) eggs. Aquaculture 239:249–259. doi: 10.1016/j.aquaculture.2004.06.003 CrossRefGoogle Scholar
  22. Celada JD, Aguilera A, Carral JM, Sáez-Royuela M, Melendre PM (2008) Rearing tench (Tinca tinca L.) larvae on live feed (Artemia) and on two transition schedules from live to dry diets. J Appl Ichthyol. doi: 10.1111/j.1439-0426.2008.01078.x Google Scholar
  23. Cukerzis JM (1964) Experiment of incubation of crayfish eggs. Akad Naut Lithuan SSR C1 33:87–93Google Scholar
  24. Cukerzis JM (1969) Larval ecology and physiology of Astacus astacus and Astacus leptodactylus. Inst Zool Bot Ac Sci Eston SSR, Tallin, pp 171–177Google Scholar
  25. Cukerzis JM (1984) La biologie de l’écrevisse (Astacus astacus L.). INRA, VersallesGoogle Scholar
  26. Cukerzis JM (1988) Astacus astacus in Europe. In: Holdich DM, Lowery RS (eds) Freshwater crayfish: biology, management and exploitation. Croom Helm, London, pp 309–340Google Scholar
  27. D’Abramo LR, Wright JS, Wright KH, Bordner CE, Conklin DE (1985) Sterol requeriments of cultured juvenile crayfish Pacifastacus leniusculus. Aquaculture 49:245–255CrossRefGoogle Scholar
  28. Edgerton BF, Owens L (1997) Age at first infection of Cherax quadricarinatus by Cherax quadricarinatus bacilliform virus and Cherax Giardivirus-like virus, and production of putative virus-free crayfish. Aquaculture 152:1–12CrossRefGoogle Scholar
  29. Edgerton BF, Owens L (1999) Histopathological surveys of the redclaw freshwater crayfish, Cherax quadricarinatus, in Australia. Aquaculture 180:23–40CrossRefGoogle Scholar
  30. Evans LH, Tsvetnenko E, Graham T, Fan A, Finn S, Knott B, Costa N (1993) Part II: artificial reproduction experiments. In: Improving commercial viability of crayfish farming. Department of Commerce and Trade, Western Australia, pp 24–52Google Scholar
  31. Gherardi F, Holdich DM (1999) Crayfish in Europe as alien species. How to make the best of a bad situation?. AA Balkema, RotterdamGoogle Scholar
  32. González J, Carral JM, Celada JD, Sáez-Royuela M, Gaudioso VR, Fernández R, López-Baissón C (1993) Management of crayfish eggs (Pacifastacus leniusculus Dana) for intensification of juvenile production. Fresw Crayfish 9:144–146Google Scholar
  33. Gydemo R, Westin L (1989) Growth and survival of juvenile Astacus astacus L. at optimized water temperature. In: de Pauw N, Jaspers E, Ackefors H, Wilkins N (eds) Aquaculture- A Biotechnology in Progress. European Aquaculture Society, Bredene, Belgium, pp 383–391Google Scholar
  34. Henryon M, Purvis IW (2000) Eggs and hatchlings of freshwater crayfish, marron (Cherax tenuimanus), can be successfully incubated artificially. Aquaculture 184:247–254CrossRefGoogle Scholar
  35. Henttonen P, Huner JV, Lindqvist OV, Henttonen L, Pitkäniemi J (1993) Moulting, growth, survival and colour of Astacus astacus (L.) juveniles fed diets with and without green plant material and maintained in individual cages and communal tanks. Freshw Crayfish 9:426–441Google Scholar
  36. Hobbs HH Jr (1988) Crayfish distribution, adaptive radiation and evolution. In: Holdich DM, Lowery RS (eds) Freshwater crayfish: biology, management and exploitation. Croom Helm, London, pp 52–82Google Scholar
  37. Hofmann J (1978) Cangrejos de río. Compañía Editorial Continental, BarcelonaGoogle Scholar
  38. Järvenpää T (1995) Artificial incubation of crayfish eggs on moving tray (abstract). Freshw Crayfish 8:716Google Scholar
  39. King CR (1993) Eggs development time and storage for redclaw crayfish Cherax quadricarinatus von Martens. Aquaculture 109:275–280CrossRefGoogle Scholar
  40. Köksal G (1988) Astacus leptodactylus in Europe. In: Holdich DM, Lowery RS (eds) Freshwater crayfish: biology, management and exploitation. Croom Helm, London, pp 365–400Google Scholar
  41. Leonard BV, Lennard WA, Kildea D (2001) A method for testing the effectiveness of artificial incubation of eggs vs. maternal brooding in the freshwater crayfish Cherax destructor (Decapoda: Parastacidae). Aquaculture 195:299–309CrossRefGoogle Scholar
  42. Lewis SD (2002) Pacifastacus. In: Holdich DM (ed) Biology of freshwater crayfish. School of Life and Environmental Sciences, University of Nottingham, Nottingham, pp 511–540Google Scholar
  43. Mason JC (1977) Artificial incubation of crayfish eggs (Pacifastacus leniusculus Dana). Fresw Crayfish 3:119–132Google Scholar
  44. Mason JC (1979) Effects of temperature, photoperiod, substrate and shelter on survival, growth and biomass accumulation of juvenile Pacifastacus leniusculus in culture. Freshw Crayfish 4:73–82Google Scholar
  45. Matthews M, Reynolds JD (1995) The in vitro culture of crayfish eggs using a recirculating airlift incubator. Freshw Crayfish 8:300–306Google Scholar
  46. McDonnell GE, Russell D (1999) Antiseptics and disinfectants: activity, action and resistance. Clin Microbiol Rev 12:147–179PubMedGoogle Scholar
  47. McDonnell G (2007) Antisepsis, disinfection and sterilization: types, action and resistance. American Society for Microbiology, Washington, DCGoogle Scholar
  48. Melendre PM, Celada JD, Carral JM, Sáez-Royuela M, Aguilera A (2006) Effectiveness of antifungal treatments during artificial incubation of the signal crayfish eggs (Pacifastacus leniusculus Dana. Astacidae). Aquaculture 257:257–265CrossRefGoogle Scholar
  49. Melendre PM, Celada JD, Carral JM, Sáez-Royuela M, Aguilera A (2007) Effects of stage 2 juvenile removal frequency on survival rates in artificial incubation of crayfish eggs (Pacifastacus leniusculus Dana. Astacidae). J Shellfish Res 26(1):201–203CrossRefGoogle Scholar
  50. Nakata K, Matsubara H, Goshima S (2004) Artificial incubation of Japanese crayfish (Cambaroides japonicus) eggs by using a simple, easy method with a microplate. Aquaculture 230:273–279CrossRefGoogle Scholar
  51. Nylund V, Westman K (1992) Crayfish diseases and their control in Finland. Finnish Fish Res 14:107–118Google Scholar
  52. Nyström P (1994) Survival of juvenile signal crayfish (Pacifastacus leniusculus) in relation to light intensity and density. Nord J Freshw Res 69:162–166Google Scholar
  53. Paris P (1911) Essai d′incubation artificielle des oeufs d′écrevisse. Bull Soc Nat D′Aclim Fr 58:56–58Google Scholar
  54. Pérez JR, Carral JM, Celada JD, Sáez-Royuela M, Romero MP (1998a) Effects of different thermal treatments during embryonic development on the artificial incubation efficiency of crayfish (Austropotamobius pallipes Lereboullet) eggs. Control of the embryogenetic duration and implications for commercial production. Invertebr Reprod Dev 34(2–3):253–258Google Scholar
  55. Pérez JR, Carral JM, Celada JD, Sáez-Royuela M, Muñoz C, Antolín JI (1998b) Effects of stripping time on the success of the artificial incubation of freshwater crayfish, Austropotamobius pallipes (Lereboullet), eggs. Aquac Res 29:389–395Google Scholar
  56. Pérez JR, Carral JM, Celada JD, Muñoz C, Sáez-Royuela M, Antolín JI (1999) The possibilities for artificial incubation of white-clawed crayfish (Austropotamobius pallipes Lereboullet) eggs: comparison between maternal and artificial incubation. Aquaculture 170:29–35CrossRefGoogle Scholar
  57. Pérez JR, Celada JD, González J, Carral JM, Sáez-Royuela M, Fernández R (2003) Duration of egg storage at different temperatures in the astacid crayfish Pacifastacus leniusculus: critical embryonic phase. Aquaculture 219:347–354CrossRefGoogle Scholar
  58. Policar T, Kozák P, Martín J (2006) Effects of egg bath and daily removal of dead eggs on hatching success and production of stage 2 juveniles during artificial incubation in noble crayfish (Astacus astacus L.). Bull Fr Pêche Piscic 380–381:1197–1206CrossRefGoogle Scholar
  59. Reichenbach H (1886) Studien zur Entwicklungsgeschichte des Flusskrebses. Abhandlungen der Senckenbergischen Naturfors-chenden Gesellschaft 14:1–137Google Scholar
  60. Reynolds JD (2002) Growth and reproduction. In: Holdich DM (ed) Biology of freshwater crayfish. School of Life and Environmental Sciences, University of Nottingham, Nottingham, pp 152–191Google Scholar
  61. Rhodes CP (1981) Artificial incubation of the eggs of the crayfish Austropotamobius pallipes Lereboullet. Aquaculture 25:129–140CrossRefGoogle Scholar
  62. Sáez-Royuela M, Carral JM, Celada JD, Muñoz C (1995) Effects of management on survival and growth of stage 2 juvenile freshwater crayfish (Pacifastacus leniusculus Dana) under laboratory conditions. Aquaculture 133:123–133CrossRefGoogle Scholar
  63. Sáez-Royuela M, Carral JM, Celada JD, Muñoz C, Pérez JR (1996) Modified photoperiod and light intensity influence on survival and growth of stage 2 juvenile signal crayfish Pacifastacus leniusculus. J Appl Aquac 6(3):33–37CrossRefGoogle Scholar
  64. Sáez-Royuela M, Carral JM, Celada JD, Pérez JR (2001) Effects of shelter type and food supply frequency on survival and growth of stage 2 juvenile white-clawed crayfish (Austropotamobius pallipes Lereboullet) under laboratory conditions. Aquacult Int 9:489–497CrossRefGoogle Scholar
  65. Sáez-Royuela M, Carral JM, Celada JD, Pérez JR, González A (2007) Live feed as supplement from the onset of external feeding of juvenile signal crayfish (Pacifastacus leniusculus Dana. Astacidae) under controlled conditions. Aquaculture 269:321–327CrossRefGoogle Scholar
  66. Savolainen R, Ruohonen K, Tulonen J (2003) Effects of bottom substrate and presence of shelter in experimental tanks on growth and survival of signal crayfish, Pacifastacus leniusculus (Dana) juveniles. Aquac Res 34(4):289–297CrossRefGoogle Scholar
  67. Savolainen R, Ruohonen K, Railo E (2004) Effect of stocking density on growth, survival and cheliped injuries of stage 2 juvenile signal crayfish Pacifastacus leniusculus Dana. Aquaculture 231:237–248CrossRefGoogle Scholar
  68. Stephensona H, Gabela M, Barnesb ME (2003) Microbial inhibition in response to treatments of hydrogen peroxide and formalin on landlocked fall chinook salmon eyed eggs, as determined by scanning electron microscopy. N Am J Aquac 65:324–329CrossRefGoogle Scholar
  69. Strempel KM (1973) Edelkrebserbrütung in Zuger-Gläsern und Anfütterung der Krebsbrut. Fresw Crayfish 1:234–238Google Scholar
  70. Taugbøl T, Skurdal J (1992) Growth, mortality and molting rate of noble crayfish Astacus astacus L. juveniles in aquaculture experiments. Aquac Fish Manag 23:411–420Google Scholar
  71. Taylor CA (2002) Taxonomy and conservation of native crayfish stocks. In: Holdich DM (ed) Biology of freshwater crayfish. School of Life and Environmental Sciences, University of Nottingham, Nottingham, pp 236–257Google Scholar
  72. Van Stappen G (1996) Use of cysts. In: Lavens P, Sorgeloos P (eds) Manual on the production and use of live food for aquaculture. FAO Fisheries Technical Paper 361. FAO, Rome, pp 107–136Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Rocío González
    • 1
  • Jesus D. Celada
    • 1
  • Vanesa García
    • 1
  • Álvaro González
    • 1
  • José M. Carral
    • 1
  • María Sáez-Royuela
    • 1
  1. 1.Departamento de Producción AnimalUniversidad de LeónLeónSpain

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