Peroxidase mediated decolorization and remediation of wastewater containing industrial dyes: a review

  • Qayyum Husain
Review paper


In this article an effort has been made to review literature based on the role of peroxidases in the treatment and decolorization of a wide spectrum aromatic dyes from polluted water. Peroxidases can catalyze degradation/transformation of aromatic dyes either by precipitation or by opening the aromatic ring structure. Peroxidases from plant sources; horseradish, turnip, tomato, soybean, bitter gourd, white radish and Saccharum uvarum and microbial sources; lignin peroxidases, manganese peroxidases, vanadium haloperoxidases, versatile peroxidases, dye decolorizing peroxidases have been employed for the remediation of commercial dyes. Soluble and immobilized peroxidases have been successfully exploited in batch as well as in continuous processes for the treatment of synthetic dyes with complex aromatic molecular structures present in industrial effluents at large scale. However, recalcitrant dyes were also decolorized by the action of peroxidases in the presence of redox mediators.


Peroxidases Decolorization Remediation Aromatic dyes Industrial effluent Color 



Horseradish peroxidase


Bitter gourd peroxidase


Bromophenol blue

Con A

Concanavalin A


Chemical oxygen demand


Dye decolorizing peroxidase




Lignin peroxidase


Manganese peroxidase

MP 11

Microperoxidase 11


Molecular weight


Recombinant dye decolorizing peroxidase


Remazol brilliant blue R


Soybean peroxidase




Tomato peroxidase


Total organic carbon


Turnip peroxidase


Vanadium haloperoxidase


Veratryl alcohol


Versatile peroxidase


Violuric acid


White radish peroxidase


White rot fungi



The author is thankful to the University Grants Commission, New Delhi, Government of India for providing special grants to the Department in the form of DRS for developing infrastructure facilities.


  1. Abadulla E, Tzanov T, Costa S, Robra KH, Cavaco-Paulo A, Gubitz GM (2000) Decolorization and detoxification of textile dyes with a laccase from Trametes hirsuta. Appl Environ Microbiol 66:3357–3362Google Scholar
  2. Acemioglu B (2004) Adsorption of Congo red from aqueous solution onto calcium-rich fly ash. J Colloid Interf Sci 274:371–379Google Scholar
  3. Adams CD, Grog S (2002) Effect of pH and gas-phase ozone concentration on the decolorization of common textile dyes. J Environ Eng 128:293–298Google Scholar
  4. Akhtar S, Husain Q (2006) Potential applications of immobilized bitter gourd (Momordica charantia) peroxidase in the removal of phenols from polluted water. Chemosphere 65:1228–1235Google Scholar
  5. Akhtar S, Khan AA, Husain Q (2005a) Potential of immobilized bitter gourd (Momordica charantia) peroxidases in the decolorization and removal of textile dyes from polluted wastewater and dyeing effluent. Chemosphere 60:291–301Google Scholar
  6. Akhtar S, Khan AA, Husain Q (2005b) Partially purified bitter gourd (Momordica charantia) peroxidase catalyzed decolorization of textile and other industrially important dyes. Bioresour Technol 96:1804–1811Google Scholar
  7. Aksu Z, Tezer S (2005) Biosorption of reactive dyes on the green alga Chlorella Vulgaris. Process Biochem 40:1347–1361Google Scholar
  8. Alam MZ, Mansor MF, Jalal KC (2009) Optimization of decolorization of methylene blue by lignin peroxidase enzyme produced from sewage sludge with Phanerocheate chrysosporium. J Hazard Mater 162(2–3):708–715Google Scholar
  9. Alcalde M, Ferrer M, Plou FJ, Ballesteros A (2006) Environmental biocatalysis: from remediation with enzymes to novel green processes. Trends Biotechnol 24:281–287Google Scholar
  10. Aleboyeh A, Moussa Y, Aleboveh H (2005) The effect of operational parameters on UV/H2O2 decolorization of Acid Blue 74. Dyes Pigm 66:129–134Google Scholar
  11. Anjaneyulu Y, Chary NS, Raj DSS (2005) Decolorization of industrial effluents-available methods and emerging technologies: a review. Rev Environ Sci Biotechnol 4:245–273Google Scholar
  12. Azmi W, Banerjee U (2001) Biological decolorization of Crystal Violet by a newly isolated Bacillus sp. and microbial assessment of toxicity of untreated and treated dye. Scientia Iranica 8:171–178Google Scholar
  13. Balchioglu IA, Arslan I, Sacan MT (2001) Homogeneous and heterogeneous advanced oxidation of two commercial reactive dyes. Environ Technol 22:813–822Google Scholar
  14. Baldrian P, Snajdr J (2006) Production of ligninolytic enzymes by litter-decomposing fungi and their ability to decolorize synthetic dyes. Enzyme Microb Technol 39:1023–1029Google Scholar
  15. Banat IM, Nigam P, Singh D, Marchant R (1996) Microbial decolorization of textile-dye-containing effluents: a review. Bioresour Technol 58:217–227Google Scholar
  16. Basheer S, Kut ÖM, Prenosil JE, Bourne JR (2004) Development of an enzyme membrane reactor for treatment of cyanide-containing wastewaters from the food industry. Biotechnol Bioeng 41(4):465–473Google Scholar
  17. Bhole BD, Ganguly B, Madhuram A, Deshpande D, Joshi J (2004) Biosorption of Methyl Violet, basic fuchsin and their mixture using dead fungal biomass. Current Science 86:1641–1645Google Scholar
  18. Bhunia A, Durani S, Wangikar PP (2001) Horseradish peroxidase catalyzed degradation of industrially important dyes. Biotechnol Bioeng 72:562–567Google Scholar
  19. Boer CG, Obici L, de Souza CGM, Peralta RM (2004) Decolorization of synthetic dyes by solid state cultures of Lentinula (Lentinus) edodes producing manganese peroxidase as the main ligninolytic enzyme. Bioresour Technol 94:107–112Google Scholar
  20. Borchert M, Libra JA (2001) Decolorization of reactive dyes by the white rot fungus Trametes versicolor in sequencing batch reactors. Biotechnol Bioeng 75:313–321Google Scholar
  21. Bousher A, Shen X, Edyvean RGJ (1997) Removal of colored organic matter by adsorption onto low cost waste materials. Water Res 31:2084–2092Google Scholar
  22. Bras R, Gomes A, Ferra MIA, Pinheiro HM, Goncalves IC (2005) Monoazo and diazo dye decolorization studies in a methanogenic UASB reactor. J Biotechnol 115:57–66Google Scholar
  23. Bulc TG, Ojstrsek A (2008) The use of constructed wetland for dye-rich textile wastewater treatment. J Hazard Mater 155:76–82Google Scholar
  24. Bumpus JA, Brock BJ (1988) Biodegradation of Crystal Violet by the white rot fungus Phanerochaete chrysosporium. Appl Environ Microbiol 54:1143–1150Google Scholar
  25. Camarero S, Ibarra D, Martίnez MJ, Martίnez AT (2005) Lignin derived-compounds as efficient laccase mediators for decolorization of different types of recalcitrant dyes. Appl Environ Microbiol 71:1775–1784Google Scholar
  26. Chagas EP, Durrant LR (2001) Decolorization of azo dyes by Phanerochaete chrysosporium and Pleurotus sajorcaju. Enzyme Microb Technol 29:473–477Google Scholar
  27. Chen KC, Wu JY, Liou DJ, Hwang SCJ (2003) Decolorization of the textile dyes by newly isolated bacterial strains. J Biotechnol 101:57–68Google Scholar
  28. Cheng X, Jia R, Li P, Tu S, Zhu Q, Tang W, Li X (2007) Purification of a new manganese peroxidase of the white-rot fungus Schizophyllum sp. F17, and decolorization of azo dyes by the enzyme. Enzyme Microb Technol 41:258–264Google Scholar
  29. Christian V, Shrivastava R, Novotny C, Vyas BR (2003) Decolorization of sulfonaphthalein dyes by manganese peroxidases activity of the white-rot fungus Phanerochaete chrysosporium. Folia Microbiol (Praha) 48:771–774Google Scholar
  30. Chung KT, Fulk GE, Egan M (1978) Reduction of azo dyes by intestinal anaerobes. Appl Environ Microbiol 35:558–562Google Scholar
  31. Cohen R, Persky L, Hazan-Eitan Z, Yarden O, Hadar Y (2002) Mn2+ alters peroxidases profiles and lignin degradation by the white-rot fungus Pleurotus ostreatus under different nutritional and growth conditions. Appl Biochem Biotechnol 102–103:415–429Google Scholar
  32. Couto SR, Sanroman MA (2007) The effect of violuric acid on the decolourization of recalcitrant dyes by laccase from Trametes hirsute. Dyes Pigm 74:123–126Google Scholar
  33. Daneshvar N, Ayazloo M, Khataee AR, Pourhassan M (2007) Biological decolorization of dye solution containing Malachite Green by microalgae Cosmarium sp. Bioresour Technol 98:1176–1182Google Scholar
  34. Dascalu T, Acosta-Ortiz ES, Morales OM, Compean I (2000) Removal of the indigo colour by laser beam-denian interaction. Opt Lasers Eng 34:179–189Google Scholar
  35. Dominguez JR, Beltran J, Rodriguez O (2005) Vis and UV photocatalytic detoxification methods (using TiO2, TiO2/H2O2, TiO2/O3, TiO2/S2O8 2−), O3, H2O2, S2O8 2−), Fe3+/H2O2 and Fe3+/H2O2/C2O4 2−) for dyes treatment. Catal Today 101:389–395Google Scholar
  36. Duarte-Vazquez MA, Ortega-Tovar MA, García-Almendárez BE, Regalado C (2002) Removal of aqueous phenolic compounds from a model system by oxidative polymerization with turnip (Brassica napus L. var. purple top white globe) peroxidase. J Chem Technol Biotechnol 78:42–47Google Scholar
  37. Duarte-Vazquez MA, Whitaker JR, Rojo-Dominguez A, Garcia-Almendárez BE, Regalado C (2003) Isolation and thermal characterization of an acidic isoperoxidase from turnip roots. J Agric Food Chem 51:5096–5102Google Scholar
  38. Duran N, Esposito E (2000) Potential applications of oxidative enzymes and phenoloxidase-like compounds in wastewater and soil treatment. Rev Appl Catal B Environ 28:83–99Google Scholar
  39. Duran N, Rosa MA, D’Annibale A, Gianfreda L (2002) Applications of laccase and tyrosinases (phenoloxidases) immobilized on different supports: a review. Enzyme Microb Technol 31:907–931Google Scholar
  40. Eichlerova I, Homolka L, Nerud F (2006a) Synthetic dye decolorization capacity of white rot fungus Dichomitus squalens. Bioresour Technol 97:2153–2159Google Scholar
  41. Eichlerova I, Homolka L, Nerud F (2006b) Ability of industrial dyes decolorization and ligninolytic enzymes production by different Pleurotus species with special attention on Pleurotus calyptratus, strain CCBAS 461. Process Biochem 41:941–946Google Scholar
  42. Ferreira-Leitao VS, Godinho da Silva J, Bon EPS (2003) Methylene Blue and Azure B oxidation by horseradish peroxidase: a comparative evaluation of class II and class III peroxidases. Applied Catalysis B: Environmental 42:213–221Google Scholar
  43. Ferreira-Leitao VS, de Carvalho MEA, Bon EPS (2007) Lignin peroxidase efficiency for Methylene Blue decolouration: comparison to reported methods. Dyes Pigm 74:230–236Google Scholar
  44. Frank MJW, Westerink JB, Schokker A (2002) Recycling of industrial wastewater by using a two step nanofiltration process for the removal of colour. Desalination 145:69–74Google Scholar
  45. Fruhwirth G, Paar A, Gudelj M, Cavaco-Paulo A, Robra KH, Gubitz G (2002) An immobilised catalase peroxidase from the alkalothermophilic Bacillus SF for the treatment of textile-bleaching effluent. Appl Microbiol Biotechnol 60:313–319Google Scholar
  46. Ghioureliotis M (1997) Assessment of residual by-product from the enzyme-catalyzed polymerization of aqueous phenolic compound. M.Sc. Thesis, Department of Civil Engineering and Applied Mechanics, McGill UniversityGoogle Scholar
  47. Ghodake GS, Kalme SD, Jadhav JP, Govindwar SP (2009) Purification and partial characterization of lignin peroxidase from Acinetobacter calcoaceticus NCIM 2890 and its application in decolorization of textile dyes. Appl Biochem Biotechnol 152(1):6–14Google Scholar
  48. Gianfreda L, Rao MA (2004) Potential of extracellular enzymes in remediation: a review. Enzyme Microb Technol 35:339–354Google Scholar
  49. Gold MH, Youngs HL, Gelpke MDS (2000) Manganese peroxidase. In: Sigel A, Sigel H (eds) Metal ions in biological systems. Marcel Dekker Inc, New York, pp 559–586Google Scholar
  50. Gomez-Toribio V, Martínez AT, Martínez MJ (2001) Oxidation of hydroquinones by the versatile ligninolytic peroxidase from Pleurotus eryngii. Eur J Biochem 268:4787–4793Google Scholar
  51. Gonzalez PS, Capozucca CE, Tigier HA, Milrad SR, Agostini E (2006) Phytoremediation of phenol from wastewater, by peroxidases of tomato hairy root cultures. Enzyme Microb Technol 39:647–653Google Scholar
  52. Gottlieb A, Shaw C, Smith A, Wheatley A, Forsythe S (2003) The toxicity of textile reactive azo dyes after hydrolysis and decolourisation. J Biotechnol 101:49–56Google Scholar
  53. Grzechulska J, Morowski WA (2002) Photocatalytic decomposition of azo dye Acid Black 1 in water over modified titanium dioxide. Appl Catal B Environ 36:45–51Google Scholar
  54. Hai FI, Yamamoto K, Fukushi K (2007) Hybrid treatment systems for dye wastewaters. Crit Rev Environ Sci Technol 37:315–377Google Scholar
  55. Hao OJ, Kim H, Chiang P-C (2000) Decolorization of wastewater. Crit Rev Environ Sci Technol 30:449–505Google Scholar
  56. Harazono K, Nakamura K (2005) Decolorization of mixtures of different reactive textile dyes by the white-rot basidiomycete Phanerochaete sordida and inhibitory effect of polyvinyl alcohol. Chemosphere 59:63–68Google Scholar
  57. Heinfling A, Bergbauer M, Szewzyk U (1997) Biodegradation of azo and phthalocynanide dyes by Trametes versicolor and Bjerkandera adusta. Appl Microbiol Biotechnol 48:261–266Google Scholar
  58. Heinfling A, Martínez MJ, Martínez AT, Bergbauer M, Szewzyk U (1998a) Purification and characterization of peroxidases from the dye-decolorizing fungus Bjerkandera adusta. FEMS Microbiol Lett 165:43–50Google Scholar
  59. Heinfling A, Martínez MJ, Martínez AT, Bergbauer M, Szewzyk U (1998b) Transformation of industrial dyes by manganese peroxidase from Bjerkandera adusta and Pleurotus eryngii in a manganese-independent reaction. Appl Environ Microbiol 64:2788–2789Google Scholar
  60. Heinfling A, Ruiz-Duenas FJ, Martínez MJ, Bergbauer M, Szewzyk U, Martínez AT (1998c) A study on reducing substrates of manganese-oxidizing peroxidases from Pleurotus eryngii and Bjerkandera adusta. FEBS Lett 428:141–146Google Scholar
  61. Held C, Kandelbauer A, Schroeder M, Cavaco-Paulo A, Guebitz GM (2005) Biotransformation of phenolics with laccase containing bacterial spores. Environ Chem Lett 3:74–77Google Scholar
  62. Hofrichter M (2002) Review: lignin conversion by manganese peroxidase (MnP). Enzyme Microb Technol 30:454–466Google Scholar
  63. Husain Q (2006) Potential applications of the oxidoreductive enzymes in the decolorization and detoxification of textile and other synthetic dyes from polluted water: a review. Crit Rev Biotechnol 60:201–221Google Scholar
  64. Husain M, Husain Q (2008) Applications of redox mediators in the treatment of organic pollutants by using oxidoreductive enzymes: a review. Crit Rev Environ Sci Technol 38:1–41Google Scholar
  65. Husain Q, Jan U (2000) Detoxification of phenols and aromatic amines from polluted wastewater by using phenol oxidases. A review. J Sci Ind Res 59:286–293Google Scholar
  66. Husain Q, Kulshrestha Y (2009) Removal of colored compounds from textile carpet industrial effluents by using immobilized turnip (Brassica rapa) and tomato (Lycopersicon esculentum) peroxidases. Water Sci Technol (in press)Google Scholar
  67. Husain Q, Husain M, Kulshrestha Y (2009) Remediation and treatment of organo-pollutants mediated by peroxidases: a review. Crit Rev Biotechnol 29(2):94–119Google Scholar
  68. Ince HN, Tezcanli G (2001) Reaction dyestuff degradation by combined sonolysis and ozonation. Dyes Pigm 49:145–153Google Scholar
  69. Jung J, Hyo-Yoon J, Ching HH, Lee JM (2002) Radiation treatment of secondary effluent forms a sewage treatment plant. Radiat Phys chem 65:533–537Google Scholar
  70. Kadhim H, Graham C, Barrat P, Evans CS, Rastall RA (1999) Removal of phenolic compounds in water using Coriolus versicolor grown on wheat bran. Enzyme Microb Technol 24:303–307Google Scholar
  71. Kalme SD, Parshettia GK, Jadhav SU, Govindwar SP (2007) Biodegradation of benzidine based dye, Direct Blue-6 by Pseudomonas desmolyticum NCIM 2112. Bioresour Technol 98:1405–1410Google Scholar
  72. Kapdan IK, Kargi F (2002) Biological decolorization of textile dyestuff containing wastewater by Coriolus versicolor in a rotation biological contactor. Enzyme Microb Technol 30:195–199Google Scholar
  73. Karam J, Nicell JA (1997) Potential application of enzymes in wastewater treatment. J Chem Technol Biotechnol 69:141–153Google Scholar
  74. Kariminiaae-Hamedaani H-R, Sakurai A, Sakakibara M (2007) Decolorization of synthetic dyes by a new manganese peroxidase-producing white rot fungus. Dyes Pigm 72:157–162Google Scholar
  75. Keharia H, Madamvar D (2003) Bioremediation concept for treatment of dye containing wastewater: a review. Indian J Exp Biol 41:1068–1075Google Scholar
  76. Kim SJ, Shoda M (1999) Purification and characterization of a novel peroxidase from Geotrichum candidum Dec 1 involved in decolorization of dyes. Appl Environ Microbiol 65:1029–1035Google Scholar
  77. Kim GY, Lee KB, Cho SH, Shim J, Moon SH (2005) Electroenzymatic degradation of azo dye using an immobilized peroxidase enzyme. J Hazard Mater 126:183–188Google Scholar
  78. Kirby N, Merchant R, McMullan G (2000) Decolorization of synthetic textile dyes by Phlebia tremellosa. FEMS Microbiol Lett 188:93–96Google Scholar
  79. Knutson K, Kirzan S, Ragauskas A (2005) Enzymatic biobleaching of two recalcitrant paper dyes with horseradish and soybean peroxidase. Biotechnology Letters 27:753–758Google Scholar
  80. Kokol V, Doliska A, Eichlerova I, Baldrian P, Nerud F (2007) Decolorization of textile dyes by whole cultures of Ischnoderma resinosum and by purified laccase and Mn-peroxidase. Enzyme Microb Technol 40:1673–1677Google Scholar
  81. Krcmar P, Ulrich R (1998) Degradation of polychlorinated biphenyl mixtures by the lignin-degrading fungus Phanerochaete chrysosporium. Folia Microbiol (Praha) 43:79–84Google Scholar
  82. Kulshrestha Y, Husain Q (2007) Decolorization and degradation of acid dyes mediated by partially purified turnip (Brassica rapa) peroxidase. Toxicol Environ Chem 89:255–267Google Scholar
  83. Lan J, Huang X, Hu M, Li Y, Qu Y, Gao P, Wu D (2006) High efficient degradation of dyes with lignin peroxidase coupled with glucose oxidase. J Biotechnol 123:483–490Google Scholar
  84. Liu JZ, Wang TL, Ji LN (2006) Enhanced dye decolorization efficiency by citraconic anhydride-modified horseradish peroxidase. J Mol Catal B Enzym 41:81–86Google Scholar
  85. Liu G, Zhou J, Qu Y, Ma X (2007) Decolorization of sulfonated azo dyes with two photosynthetic bacterial strains and a genetically engineered Escherichia coli strain. World J Microbiol Biotechnol 23:931–937Google Scholar
  86. Lopez C, Mielgo I, Moreira MT, Feijoo G, Lema JM (2002) Enzymatic membrane reactors for biodegradation of recalcitrant compounds: application to dye decolourisation. J Biotechnol 99:249–257Google Scholar
  87. Lopez C, Moreira MT, Feijoo G, Lema JM (2004) Dye decolorization by manganese peroxidase in an enzymatic membrane bioreactor. Biotechnol Prog 20:74–81Google Scholar
  88. Lopez C, Moreira MT, Feijoo G, Lema JM (2007) Dynamic modelling of an enzymatic membrane reactor for the treatment of xenobiotic compounds. Biotechnol Bioeng 97:1128–1137Google Scholar
  89. Machado KMG, Matheus DR, Bononi VLR (2005) Ligninolytic enzymes production and Remazol Brilliant Blue R decolorization by tropical Brazilian basidiomycetes fungi. Braz J Microbiol 36:246–252Google Scholar
  90. Maddhinni VL, Vurimindi HB, Yerramilli A (2006) Degradation of azo dye with horseradish peroxidase (HRP). J Indian Inst Sci 86:507–514Google Scholar
  91. Mao X, Buchanan DI, Stanley JS (2006) Phenol removal from aqueous solution by fungal peroxidases. J Environ Eng Sci 5:103–109Google Scholar
  92. Martinez AT (2002) Molecular biology and structure function of lignin-degrading lignin-degrading heme peroxidases. Enzyme Microb Technol 30:425–444Google Scholar
  93. Matthew T, Bumpus JA (1998) Biodegradation of Congo Red by Phanerochaete chrysosporium. Water Res 32:1713–1717Google Scholar
  94. Matto M, Husain Q (2007) Decolorization of direct dyes by salt fractionated turnip proteins enhanced in the presence of hydrogen peroxide and redox mediators. Chemosphere 69(2):338–345Google Scholar
  95. Matto M, Husain Q (2008) Redox mediated decolorization of Direct Red 23 and Direct Blue 80 catalyzed by bioaffinity based immobilized tomato (Lycopersicon esculentum) peroxidases. Biotechnol J 3(8):1224–1231Google Scholar
  96. Matto M, Husain Q (2009a) Decolorization of direct dyes by immobilized turnip peroxidase in batch and continuous processes. Ecotoxicol Environ Saf 72:963–971Google Scholar
  97. Matto M, Husain Q (2009b) Decolorization of textile effluent by bitter gourd peroxidase immobilized on concanavalin A layered calcium alginate-starch beads. J Hazard Mater 164:1540–1546Google Scholar
  98. Matto M, Satar R, Husain Q (2009) Application of calcium alginatestarch entrapped bitter gourd (momordica charantia) peroxidase for the removal of colored compounds from a textile effluent in batch as well as in continuous reactor. Appl Biochem Biotech. doi: 10.1007/s12010-008-8396-8
  99. Mbuligwe SE (2005) Comparative treatment of dye-rich wastewater in engineered wetland systems (EWSs) vegetated with different plants. Water Res 39:271–280Google Scholar
  100. McMullan G, Meehan C, Conneely A, Kirby N, Robinson T, Nigam P, Banat IM, Marchant R, Smyth WF (2001) Microbial decolourisation and degradation of textile dyes. Appl Microbiol Biotechnol 56:81–87Google Scholar
  101. Mester T, Field JA (1998) Characterization of a novel manganese peroxidase-lignin peroxidase hybrid isozyme produced by Bjerkandera species strain BOS55 in the absence of manganese. J Biol Chem 273:15412–15417Google Scholar
  102. Michniewicz A, Ledakowicz S, Ullrich R, Hofrichter M (2008) Kinetics of the enzymatic decolorization of textile dyes by laccases from Cerrena unicolor. Dyes Pigm 77:295–302Google Scholar
  103. Mielgo I, Moreira MT, Feijoo G, Lema JM (2001) A packed-bed fungal bioreactor for the continuous decolorization of azo dyes (Orange II). J Biotechnol 89:99–106Google Scholar
  104. Mielgo I, Lopez C, Moreira MT, Feijoo G, Lema JM (2003) Oxidative degradation of azo dyes by manganese peroxidase under optimized conditions. Biotechnol Prog 19:325–331Google Scholar
  105. Mohan SV, Roa CN, Prasad KK, Karthikeyan J (2002) Treatment of simulated Reactive Yellow 22 (Azo) dye effluents using Spirogyra species. Waste Manage 22:575–582Google Scholar
  106. Mohan SV, Prasad KK, Rao NC, Sarma PN (2005) Acid azo dye degradation by free and immobilized horseradish peroxidase catalyzed process. Chemosphere 58:1097–1105Google Scholar
  107. Mohorcic M, Teodorovic S, Golob V, Friedrich J (2006) Fungal and enzymatic decolorisation of artificial textile dye baths. Chemosphere 63:1709–1717Google Scholar
  108. Moreira MT, Palma C, Mielgo I, Feijoo G, Lema JM (2001) In vitro degradation of a polymeric dye (Poly R-478) by manganese peroxidase. Biotechnol Bioeng 75:362–368Google Scholar
  109. Moreira PR, Duez C, Dehareng D, Antunes A, Almeida-Vala E, Frere JM, Malcata FX, Duarte JC (2005) Molecular characterization of a versatile peroxidase from a Bjerkandera strain. J Biotechnol 118:339–352Google Scholar
  110. Moreira PR, Bouillenne F, Almeida-Vara E, Malcata FX, Frère JM, Duarte JC (2006) Purification, kinetics and spectral characterisation of a new versatile peroxidase from a Bjerkandera sp. isolate. Enzyme Microb Technol 38:28–33Google Scholar
  111. Muthukumar M, Sargunamani D, Selvakumar N (2005) Statistical analysis of the effect of aromatic, azo and sulphonic acid groups on decolouration of acid dye effluents using advanced oxidation processes. Dyes Pigm 65:151–158Google Scholar
  112. Mutlu SH, Yetis V, Gurkan T, Yilmaz L (2002) Decolorization of wastewater of a baker’s yeast plant by membrane processes. Water Res 36:609–616Google Scholar
  113. Nazari K, Esmaeili N, Mahmoudi A, Rahimi H, Moosavi-Movahedi AA (2007) Peroxidative phenol removal from aqueous solutions using activated peroxidase biocatalyst. Enzyme Microb Technol 41:226–233Google Scholar
  114. Nigam AG, Banat IM, Singh D, Marchant R (2000) Physical removal of textile dyes from effluents and solid state fermentation by dye-adsorbed agricultural residues. Bioresour Technol 72:219–226Google Scholar
  115. Nyanhongo GS, Gomesa J, Gubitz GM, Zvauya R, Read J, Steiner W (2002) Decolorization of textile dyes by laccases from a newly isolated strain of Trametes modesta. Water Res 36:1449–1456Google Scholar
  116. O’Neill C, Hawkes FR, Hawkes DL, Lourenco ND, Pinheiro HM, Delee W (1999) Colour on textile effluents-source, measurement, discharge contents and simulation: a review. J Chem Technol Biotechnol 74:1009–1018Google Scholar
  117. Okazaki S-Y, Nagasawa S-I, Goto M, Furusaki S, Wariishi H, Tanaka H (2002) Decolorization of azo and anthraquinone dyes in hydrophobic organic media using microperoxidase-11 entrapped in reversed micelles. Biochem Eng J 12:237–241Google Scholar
  118. Padmesh TVN, Vijayaraghavan K, Sekaran G, Velan M (2005) Batch and column studies on biosorption of acid dyes on fresh water macro alga Azolla filiculoides. J Hazard Mater 125:121–129Google Scholar
  119. Park C, Lee M, Lee B, Kim SW, Chase HA, Lee J, Kim S (2007) Biodegradation and biosorption for decolorization of synthetic dyes by Funalia trogii. Biochem Eng J 36:59–65Google Scholar
  120. Parshetti G, Kalme S, Saratale G, Govindwar S (2006) Biodegradation of Malachite Green by Kocuria rosea MTCC 1532. Acta Chim Slov 53:492–498Google Scholar
  121. Passardi F, Bakalovic N, Teixeira FK, Margis-Pinheiro M, Penel C, Dunand C (2007) Prokaryotic origins of the non-animal peroxidase superfamily and organelle-mediated transmission to eukaryotes. Genomics 89:567–579Google Scholar
  122. Pearce CI, Lloyd JR, Guthrie JT (2003) The removal of colour from textile wastewater using whole bacterial cells: a review. Dyes Pigm 58:179–196Google Scholar
  123. Pisklak TJ, Macías M, Coutinho DH, Huang RS, Balkus KJ (2006) Hybrid materials for immobilization of MP-11 catalyst. Top Catal 38:269–278Google Scholar
  124. Pointing SB (2001) Feasibility of bioremediation by white-rot fungi. Appl Microbiol Biotechnol 57:20–33Google Scholar
  125. Pointing SB, Vrijmoed LLP (2000) Decolorization of azo and triphenylmethane dyes by Pycnoporus sanguineus producing laccase as the sole phenoloxidase. World J Microbiol Biotechnol 16:317–318Google Scholar
  126. Pricelius S, Held C, Sollner S, Deller S, Murkovic M, Ullrich R, Hofrichter M, Cavaco-Paulo A, Macheroux P, Guebitz GM (2007) Enzymatic reduction and oxidation of fibre-bound azo-dyes. Enzyme Microb Technol 40:1732–1738Google Scholar
  127. Rai HS, Bhattacharyya MS, Singh J, Bansal TK, Vats P, Banerjee UC (2005) Removal of dyes from the effluent of textile and dyestuff manufacturing industry: a review of emerging techniques with reference to biological treatment. Crit Rev Environ Sci Technol 35:219–238Google Scholar
  128. Regalado C, Garcia-Almendarcz BE, Duarte-Vazquez MA (2004) Biotechnological applications of peroxidases. Phytochem Rev 3:243–256Google Scholar
  129. Robinson T, McMullan G, Marchant R, Nigam P (2001) Remediation of dyes in textile effluents: a critical review on current treatment technologies with a proposed alternative. Bioresour Technol 77:247–255Google Scholar
  130. Rojas-Melgarejo F, Marin-Iniesta F, Rodriguez-Lopez JN, García-Cánovas F, García-Ruiz PA (2006) Cinnamic carbohydrate esters show great versatility as supports for the immobilization of different enzymes. Enzyme Microb Technol 38:748–755Google Scholar
  131. Rott U (2003) Multiple use of water in industry-The textile industry case. J Environ Sci Health 38:1629–1639Google Scholar
  132. Ruiz-Duenas FJ, Camarero S, Perez-Boada M, Martínez MJ, Martínez AT (2001) A new versatile peroxidase from Pleurotus. Biochem Soc Trans 29:116–122Google Scholar
  133. Ryan S, Schnitzhofer W, Tzanov T, Cavaco-Paulo A, Gubitz GM (2003) An acid-stable laccase from Sclerotium rolfsii with potential for wool dye decolourization. Enzyme Microb Technol 33:766–774Google Scholar
  134. Sanghi R, Dixit A, Guha S (2006) Sequential batch culture studies for the decolorization of reactive dye by Coriolus versicolor. Bioresour Technol 97:396–400Google Scholar
  135. Satar R, Husain Q (2009a) Bitter gourd peroxidase-catalyzed degradation and decolorization of water insoluble disperse dyes mediated by 1-hydroxybenzotrialzole. Biotechnol Bioprocess Eng 14:213–219Google Scholar
  136. Satar R, Husain Q (2009b) Phenol mediated decolorization and removal of disperse dyes by bitter gourd (Momordica charantia) peroxidase. Environ Technol (in press)Google Scholar
  137. Satar R, Husain Q (2009c) Applications of Celite-adsorbed white radish (Raphanus sativus) peroxidase in batch process and continuous reactor for the degradation of reactive dyes. Biochem Eng J 46(2):96–104Google Scholar
  138. Sato T, Hara S, Matsui T, Sazaki G, Saijo S, Ganbe T, Tanaka N, Sugano Y, Shoda M (2004) A unique dye-decolorizing peroxidase, DyP, from Thanatephorus cucumeris Dec 1: heterologous expression, crystallization and preliminary X ray analysis. Acta Crystallogr A 60:149–152Google Scholar
  139. Schlosser D, Hofer C (2002) Laccase-catalyzed oxidation of Mn2+ in the presence of natural Mn3+ chelators as a novel source of extracellular H2O2 production and its impact on manganese peroxidase. Appl Environ Microbiol 68:3514–3521Google Scholar
  140. Schuller DJ, Ban N, van Huystee RB, McPherson A, Poulos TL (1996) The crystal structure of peanut peroxidase. Structure 4:311–321Google Scholar
  141. Sedighi M, Karimi A, Vahabzadeh F (2009) Involvement of ligninolytic enzymes of Phanerochaete chrysosporium in treating the textile effluent containing Astrazon Red FBL in a packed-bed bioreactor. J Hazard Mater 169(1–3):88–93Google Scholar
  142. Selvam K, Swaminathan K, Chae KS (2003) Decolourization of azo dyes and a dye industry effluent by a white rot fungus Thelephora sp. Bioresour Technol 88:115–119Google Scholar
  143. Semple KT, Cain RB, Schmidt S (1999) Biodegradation of aromatic compounds by microalgae. FEMS Microbiol Lett 170:291–300Google Scholar
  144. Shaffiqu TS, Roy JJ, Nair RA, Abraham TE (2002) Degradation of textile dyes mediated by plant peroxidases. Appl Biochem Biotechnol 102–103:315–326Google Scholar
  145. Shakeri M, Shoda M (2007) Change in turnover capacity of crude recombinant dye-decolorizing peroxidase (rDyP) in batch and fed-batch decolorization of Remazol Brilliant Blue R. Appl Microbiol Biotechnol 76:919–926Google Scholar
  146. Shakeri M, Shoda M (2008) Decolorization of an anthraquinone dye by the recombinant dye-decolorizing peroxidase (rDyP) immobilized on mesoporous materials. J Mol Catal B Enzym 54:42–49Google Scholar
  147. Shakeri M, Sugano Y, Shoda M (2008) Stable repeated-batch production of recombinant dye-decolorizing peroxidase (rDyP) from Aspergillus oryzae. J Biosci Bioeng 105(6):683–686Google Scholar
  148. Shim J, Kim GY, Yeon KH, Cho S-H, Woo JJ, Moon S-H (2007) Degradation of azo dye by an electroenzymatic method using horseradish peroxidase immobilized on porous support. Korean J Chem Eng 24:72–78Google Scholar
  149. Shin KS (2004) The role of enzymes produced by white-rot fungus Irpex lacteus in the decolorization of the textile industry effluent. J Microbiol 42:37–41Google Scholar
  150. Shrivastava R, Christian V, Vyas BRM (2005) Enzymatic decolorization of sulfonaphthalein dyes. Enzyme Microb Technol 36:333–337Google Scholar
  151. Stolz A (2001) Basic and applied aspects in the microbial degradation of azo dyes. Appl Microbiol Biotechnol 56:69–80Google Scholar
  152. Sugano Y, Nakano R, Sasaki K, Shoda M (2000) Efficient heterologous expression in Aspergillus oryzae of a unique dye-decolorizing peroxidase, DyP, of Geotrichum candidum Dec 1. Appl Environ Microbiol 66:1754–1758Google Scholar
  153. Sugano Y, Matsushima Y, Shoda M (2006) Complete decolorization of the anthraquinone dye Reactive Blue 5 by the concerted action of two peroxidases from Thanatephorus cucumeris Dec 1. Appl Microbiol Biotechnol 73:862–871Google Scholar
  154. Sugimori D, Banzawa R, Kurozumi M, Okura I (1999) Removal of disperse dyes by the fungus Cunninghamella polymorpha. J Biosci Bioeng 87:252–254Google Scholar
  155. Sumathi S, Manju BS (2000) Uptake of reactive textile dyes by Aspergillus foetidus. Enzyme Microb Technol 27:347–355Google Scholar
  156. Susla M, Novotný C, Erbanová P, Svobodová K (2008) Implication of Dichomitus squalens manganese-dependent peroxidase in dye decolorization and cooperation of the enzyme with laccase. Folia Microbiol (Praha) 53(6):479–485Google Scholar
  157. Svobodová K, Erbanová P, Sklenár J, Novotný C (2006) The role of Mn-dependent peroxidase in dye decolorization by static and agitated cultures of Irpex lacteus. Folia Microbiologica(Praha) 51(6):573–578Google Scholar
  158. ten Brink HB, Dekker HL, Schoemaker HE, Wever R (2000) Oxidation reactions catalyzed by vanadium chloroperoxidase from Curvularia inaequalis. J Inorg Biochem 80:91–98Google Scholar
  159. Tien M, Kirk TK (1983) Lignin-degrading enzyme from the hymenomycete Phanerochaete chrysosporium burds. Science 221:661–663Google Scholar
  160. Tinoco R, Verdin J, Vazquez-Duhalt R (2007) Role of oxidizing mediators and tryptophan 172 in the decolorization of industrial dyes by the versatile peroxidase from Bjerkandera adusta. J Mol Catal B Enzym 46:1–7Google Scholar
  161. Torres E, Bustos-Jaimes I, Le Borgne S (2003) Potential use of oxidative enzymes for the detoxification of organic pollutants. Applied Catalysis B: Environmental 46:1–15Google Scholar
  162. Ulson de Souza SMAG, Forgiarini E, Ulson de Souza AA (2007) Toxicity of textile dyes and their degradation by the enzyme horseradish peroxidase (HRP). J Hazard Mater 147:1073–1078Google Scholar
  163. Urra J, Sepulveda L, Contreras E, Palma C (2006) Screening of static culture and comparison of batch and continuous culture for the textile dye biological decolorization by Phanerochaete chrysosporium. Braz J Chem Eng 23:281–290Google Scholar
  164. Verma P, Madamwar D (2002a) Decolourization of synthetic dyes by a newly isolated strain of Serratia marcescens. World J Microbiol Biotechnol 19:615–618Google Scholar
  165. Verma P, Madamwar D (2002b) Decolorization of synthetic textile dyes by lignin peroxidase of Phanerochaete chrysosporium. Folia Microbiol (Praha) 47:283–286Google Scholar
  166. Verma P, Madamwar D (2005) Decolorization of azo dyes using basidiomycete strain PV002. World J Microbiol Biotechnol 21:481–485Google Scholar
  167. Verma P, Baldrian P, Nerud F (2003) Decolorization of structurally different synthetic dyes using cobalt(II)/ascorbic acid/hydrogen peroxide system. Chemosphere 50:975–979Google Scholar
  168. Wariishi H, Kabuto M, Mikuni J, Oyadomari M, Tanaka H (2002) Degradation of water-insoluble dyes by microperoxidase 11, an effective and stable peroxidative catalyst in hydrophilic organic media. Biotechnol Prog 18:36–42Google Scholar
  169. Wesenberg D, Buchon F, Agathos SN (2002) Degradation of dye-containing textile effluent by the agaric white-rot fungus Clitocybula dusenii. Biotechnology Letters 24:989–993Google Scholar
  170. Wesenberg D, Kyriakides I, Agathos SN (2003) White-rot fungi and their enzymes for the treatment of industrial dye effluents. Biotechnol Adv 22:161–187Google Scholar
  171. Xiao-Bin C, Jia R, Li PS, Zhu Q, Tu SQ, Tang WZ (2007) Studies on the properties and co-immobilization of manganese peroxidase. Chin J Biotechnol 23:90–95Google Scholar
  172. Yan H, Pan G (2004) Increase in biodegradation of dimethyl phthalate by Closterium lunula using inorganic carbon. Chemosphere 55:1281–1285Google Scholar
  173. Yang QX, Yang M, Pritsch K, Yediler A, Hagn A, Schloter M, Kettrup A (2003) Decolorization of synthetic dyes and production of manganese-dependent peroxidase by new fungal isolates. Biotechnology Letters 25:709–711Google Scholar
  174. Yu G, Wen X, Li R, Qian Y (2006) In vitro degradation of a reactive azo dye by crude ligninolytic enzymes from nonimmersed liquid culture of Phanerochaete chrysosporium. Process Biochem 41:1987–1993Google Scholar
  175. Zollinger H (1991) Colour chemistry: synthesis, properties and applications of organic dyes and pigments, 5th edn. VCH Publishers, Weinheim, pp 187–246Google Scholar
  176. Zouari-Mechichi H, Mechichi T, Dhouib A, Sayadi S, Martinez TA, Martinez JA (2006) Laccase purification and characterization from Trametes trogii isolated in Tunisia: decolorization of textile dyes by the purified enzyme. Enzyme Microb Technol 39:141–148Google Scholar

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© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  1. 1.Department of Biochemistry, Faculty of Life SciencesAligarh Muslim UniversityAligarhIndia

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