Advertisement

Radiochemistry

, Volume 47, Issue 3, pp 308–310 | Cite as

Comparative Study of Reaction of Atomic Tritium with Glucosamine and Amino Acids

  • G. A. Badun
  • A. L. Ksenofontov
  • E. V. Lukashina
  • V. Yu. Pozdnyakova
  • V. M. Fedoseev
Article

Abstract

Reaction of amino acids (glycine and serine) and amino sugar (glucosamine) with atomic tritium generated by thermal dissociation of molecular tritium on a tungsten filament was studied. A frozen aqueous solutions and a freeze-dried mixture of these compounds was bombarded with tritium atoms is a special vacuum unit. The relative yield of the labeled compounds was determined as influenced by the reaction conditions (residual pressure in the system and bombardment time) and target type (frozen solution and freeze-dried mixture). Formation of labeled products is almost independent of the tritium pressure. The ratio of the formation rates of labeled serine and glycine in the frozen solution and freeze-dried mixture bombarded with atomic tritium for 45–270 s was 1.66±0.15 and 1.44±0.13, respectively. At shorter reaction time (15 s), the ratio increases to 3.5±0.2 and 2.0±0.4, respectively. The formation rate of [3H]glucosamine in the mixture is higher at a shorter bombardment time. The radioactivity ratio of labeled glucosamine and glycine formed in frozen solutions and freeze-dried mixture in 15 s was 26.0±2.3 and 6.8±0.6, respectively. At longer reaction time, the relative yield of [3H]glucosamine sharply decreases owing to stronger radiolysis of labeled glucosamine on exposure to tritium beam.

Keywords

Tritium Glucosamine Relative Yield Longe Reaction Time Amino Sugar 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

REFERENCES

  1. 1.
    Filatov, E.S., Simonov, E.F., Shishkov, A.V., and Mogil’nikov, V.P., Radiokhiniya, 1979, vol. 21, no.6, pp. 909–913.Google Scholar
  2. 2.
    Orlova, M.A., Simonov, E.F., and Filatov, E.S., Khim. Vys. Energ., 1979, vol. 13, no.5, pp. 468–473.Google Scholar
  3. 3.
    Shishkov, A.V., Unukovich, M.S., Rumyantsev, Yu.M., et al., Dokl. Akad. Nauk SSSR, 1980, vol. 251, no.3, pp. 751–754.Google Scholar
  4. 4.
    Baratova, L.A., Rumyantsev, Yu.M., Simonov, E.F., et al., Khim. Vys. Energ., 1981, vol. 15, no.4, pp. 370–373.Google Scholar
  5. 5.
    Baratova, L.A., Gol’danskii, V.I., Rumyantsev, Yu.M., et al., Mol. Biol., 1982, vol. 16, no.1, pp. 117–122.Google Scholar
  6. 6.
    Asatryan, R.S., German, K.E., Filatov, E.S., and Nesmeyanov, An.N., Radiokhimiya, 1982, vol. 24, no.5, pp. 567–569.Google Scholar
  7. 7.
    Gedrovich, A.V., Gol’danskii, V.I., Rumyantsev, Yu.M., et al., Radiokhimiya, 1984, vol. 26, no.4, pp. 483–494.Google Scholar
  8. 8.
    Badun, G.A. and Filatov, E.S., Radiokhimiya, 1988, vol. 30, no.4, pp. 531–536.Google Scholar
  9. 9.
    Badun, G.A., Radiokhimiya, 1990, vol. 32, no.5, pp. 125–126.Google Scholar
  10. 10.
    Gedrovich, A.V. and Badun, G.A., Mol. Biol., 1992, vol. 26, no.3, pp. 558–563.Google Scholar
  11. 11.
    Volynskaya, A.V., Kasumov, E.A., Bogacheva, E.N., et al., Eur. Biophys. J., 1994, vol. 23, no.2, pp. 139–143.PubMedGoogle Scholar
  12. 12.
    Moser, H., Nordin, P., and Senne, J., Int. J. Appl. Radiat. Isot., 1964, vol. 15, pp. 557–559.PubMedGoogle Scholar
  13. 13.
    Ksenofontov, A.L., Zhirnov, O.P., Danilov, A.V., and Baratova, L.A., Mol. Biol., 1995, vol. 29, pp. 635–643.Google Scholar
  14. 14.
    Badun, G.A., Pozdnyakova, N.Yu., Kudryavtsev, A.V., and Perminova, I.V., Ecol. Future, Bulg. J. Ecol. Sci., 2003, vol. 2, nos.3–4, pp. 26–27.Google Scholar
  15. 15.
    Orlov, D.S., Gumusovye kisloty pochv i obshchaya teoriya gumifikatsii (Humic Acids of Soils and General Humification Theory), Moscow: Mosk. Gos. Univ., 1990.Google Scholar
  16. 16.
    Badun, G.A., Lukashina, E.V., Ksenofontov, A.L., and Fedoseev, V.M., Radiokhimiya, 2001, vol. 43, no.3, pp. 272–276.Google Scholar
  17. 17.
    Badun, G.A., Lukashina, E.V., Batuk, O.N., et al., Mater. Sci. Eng. C, 2003, vol. 23, pp. 797–802.Google Scholar

Copyright information

© MAIK “Nauka/Interperiodica” 2005

Authors and Affiliations

  • G. A. Badun
    • 1
  • A. L. Ksenofontov
    • 2
  • E. V. Lukashina
    • 2
  • V. Yu. Pozdnyakova
    • 1
  • V. M. Fedoseev
    • 1
  1. 1.Chair of Radiochemistry, Chemical FacultyLomonosov State UniversityMoscowRussia
  2. 2.Department of Chromatography, Belozerskii Research Institute of Physicochemical BiologyMoscow State UniversityMoscowRussia

Personalised recommendations