Identification and Characterization of Diploid and Tetraploid in Platycodon grandiflorum
Platycodon grandiflorum (PG), a species of herbaceous flowering perennial plant of the family Campanulaceae, has been used as a traditional oriental medicine for bronchitis, asthma, pulmonary tuberculosis, diabetes, hepatic fibrosis, bone disorders and many others similar diseases and as a food supplement. For the primary profiling of PG gas chromatography coupled with high resolution – time of flight mass spectrometry (GC/HR-TOF MS) was used as an analytical tool. A comparison of optimal extraction of metabolites was carried out with a number of solvents [hexane, methylene chloride, methanol, ethanol, methanol: ethanol (70:30, v:v)]. In extracts with methanol: ethanol (70:30 v:v) were detected higher amounts of metabolites than with other solvents. Principal component analysis (PCA) and partial least-squares discriminant analysis (PLS-DA) plots showed significant differences between the diploid and tetraploid metabolite profiles. Extracts of tetraploid showed higher amounts of amino acids, while extracts of diploid contained more organic acids and sugars.
KeywordsGas chromatography/high resolution time of flight mass spectrometry (GC/HR-TOF MS) Diploid Tetraploid Platycodon grandiflorum (PG) Partial least-squares discriminant analysis (OPLS-DA)
This research was supported by High Value-added Food Technology Development Program of IPET, Ministry of Agriculture, Food and Rural Affairs, Republic of Korea. And this research was supported by the Korea Basic Science Institute (C36917).
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Conflict of Interests
The authors declare that they have no any conflict of interests.
Human and Animal Studies
This article does not contain any studies with human or animal subjects.
The authors declare that they have no competing interests.
- 2.Choi JH, Hwang YP, Lee HS, Jeong HG (2009) Inhibitory effect of Platycodi radix on ovalbumin-induced airway inflammation in a murine model of asthma. Food Chem Toxicol 47:1272–1279Google Scholar
- 5.Lee J-Y, W-Ik H, Lima S-T (2004) Antioxidant and anticancer activities of organic extracts from Platycodon grandiflorum A. De Candolle roots. J Ethnopharm 93:409–415Google Scholar
- 8.Sung N, Lee S, Shin J, Lee I, Chung Y (1996) Effects of Platycodon grandiflorum extract on blood glucose and lipid composition in alloxan induced hyperglycemic rats. J Korean Soc Food Sci Nutr 25:986–992Google Scholar
- 9.Zheng J, He J, Ji B, Li Y, Zhang X (2007) Antihyperglycemiceffects of Platycodon grandiflorum (Jacq.) A. DC. Extract on streptozotocin-induced diabetic mice. Plant Foods Hum Nutr 62:7–11Google Scholar
- 10.Takagi K, Lee EB (1972) Pharmacological studies on Platycodon grandiflorum A. DC. 3. Activities of crude platycodin on respiratory and circulatory systems and its other pharmacological activities. Yakugaku Zasshi 92:969–973Google Scholar
- 14.Kim IH, Kim HH, Hong EY, Yun JS, Yun T, Hwang JK, Lee CH (2003) Breeding of tetraploid in Platycodon grandiflorum A. DC. By colchicine treatment. Plant Resour 6:188–194Google Scholar
- 15.Gao S, Shu L (2002) Induction and identification of autotetraploid plant of Platycodon grandiflorum. Zhong yao cai = Zhongyaocai = J Chinese Medicinal Materials 25:461–462Google Scholar
- 16.Wang X, Xiong L, Qu Y, Yang J, Gu Z (2005) The ployploid induction and identification of Platycodon grandiflorus (Campanulaceae) in China. Acta Bot Yunnanica 28:593–598Google Scholar
- 20.Abate S, Ahn YG, Kind T, Cataldi TR, Fiehn O (2010) Determination of elemental compositions by gas chromatography/time-of-flight mass spectrometry using chemical and electron ionization. RCM 24:1172–1180Google Scholar