Identification and Characterization of Diploid and Tetraploid in Platycodon grandiflorum
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Platycodon grandiflorum (PG), a species of herbaceous flowering perennial plant of the family Campanulaceae, has been used as a traditional oriental medicine for bronchitis, asthma, pulmonary tuberculosis, diabetes, hepatic fibrosis, bone disorders and many others similar diseases and as a food supplement. For the primary profiling of PG gas chromatography coupled with high resolution – time of flight mass spectrometry (GC/HR-TOF MS) was used as an analytical tool. A comparison of optimal extraction of metabolites was carried out with a number of solvents [hexane, methylene chloride, methanol, ethanol, methanol: ethanol (70:30, v:v)]. In extracts with methanol: ethanol (70:30 v:v) were detected higher amounts of metabolites than with other solvents. Principal component analysis (PCA) and partial least-squares discriminant analysis (PLS-DA) plots showed significant differences between the diploid and tetraploid metabolite profiles. Extracts of tetraploid showed higher amounts of amino acids, while extracts of diploid contained more organic acids and sugars.
KeywordsGas chromatography/high resolution time of flight mass spectrometry (GC/HR-TOF MS) Diploid Tetraploid Platycodon grandiflorum (PG) Partial least-squares discriminant analysis (OPLS-DA)
This research was supported by High Value-added Food Technology Development Program of IPET, Ministry of Agriculture, Food and Rural Affairs, Republic of Korea. And this research was supported by the Korea Basic Science Institute (C36917).
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Conflict of Interests
The authors declare that they have no any conflict of interests.
Human and Animal Studies
This article does not contain any studies with human or animal subjects.
The authors declare that they have no competing interests.
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