Antithrombotic Effects of Amaranthus hypochondriacus Proteins in Rats
Cardiovascular disease (CVD) is a major cause of disability and premature death throughout the world. Diets with antithrombotic components offer a convenient and effective way of preventing and reducing CVD incidence. The aim of the present work was to assess in vivo and ex vivo effects of Amaranthus hypochondriacus proteins on platelet plug formation and coagulation cascade. Amaranth proteins were orally administrated to rats (AG, 8 animals) and bleeding time was determined showing no significant difference compared with control rats (CG, 7 animals). However, results show a strong tendency, suggesting that amaranth proteins are involved in the inhibition of thrombus formation. Non-anticoagulated blood extracted from animals was analyzed with the hemostatometer, where AG parameters obtained were twice the values showed by CG. The clotting tests, thrombin time (TT) and activated partial thromboplastin time (APTT), presented a 17 and 14 % clotting formation increase respectively when comparing AG with CG. The ex-vivo assays confirm the hypothesis inferring that amaranth proteins are a potential antithrombotic agent.
KeywordsFunctional food Amaranth proteins Antithrombotic activity Animals assays
Rats fed with amaranth proteins
Activated partial thromboplastin time
Rats that were fed with control diet
Rats injected with heparin
This work was supported by PIP 11220110101109. We also wish to thank Mariana M. Gonzalez from Facultad de Ciencias Exactas, for helping us during the realization of the clotting tests.
Compliance with Ethical Standards
Conflict of Interest
Authors, A.C. Sabbione, G. Rinaldi, M.C. Añón, and A. Scilingo, declare that they have no conflict of interest.
All experimental procedures were in accordance with the ethical standards approved by CICUAL, Facultad de Ciencias Médicas de la Universidad Nacional de La Plata Ethics Committee. All efforts were made to minimize the number of animals used and their suffering. This article does not contain studies with human participants performed by the authors.
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