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Plant Foods for Human Nutrition

, Volume 70, Issue 3, pp 338–343 | Cite as

Growth Impairment Caused by Raw Linseed Consumption: Can Trypsin Inhibitors Be Harmful for Health?

  • Katya Anaya
  • Ana C. B. Cruz
  • Dayse C. S. Cunha
  • Sandra M. N. Monteiro
  • Elizeu A. dos Santos
Original Paper

Abstract

Linseed (Linun usitatissimum L.) is an important oilseed whose nutritional value can be impaired due to presence of antinutritional factors and low protein digestibility. Protein fractions from raw linseed meal were extracted, isolated and analyzed in vitro and in vivo. Globulins, the major protein fraction of linseed, showed low in vitro susceptibility to trypsin and chymotrypsin, but its in vivo digestibility was 93.2 %. Albumin fraction had high trypsin inhibition activity (5250 Inhibition Units g−1) and presented low molecular mass protein bands, similar to known trypsin inhibitors. Raw linseed consumption caused negative effects on rat growth and reduction of intestinal villi. Results indicate that raw linseed meal must not be used as an exclusive source of protein regardless of the major proteins have high digestibility; digestive enzymes inhibitors in raw linseed probably reduces the protein utilization.

Keywords

Linseed Trypsin inhibitors Globulins Albumins Antinutritional factors Protein digestibility 

Abbreviations

C

Chymotrypsin

GI

Group I – casein group

GII

Group II – raw linseed group

HSA

Human salivary amylase

IU

Inhibition unit

NPR

Net protein ratio

PA

Pancreatic amylase

PE

Pancreatic elastase

PER

Protein efficiency ratio

SDS-PAGE

Sodium dodecyl sulphate polyacrylamide gel electrophoresis

T

Trypsin

TD

True digestibility

Notes

Acknowledgments

Our sincere acknowledgements to Maurício Pereira de Sales, a great researcher and professor (in memoriam). The authors also gratefully acknowledge Ana Heloneida A. Morais and Adriana F. Uchôa for fruitful discussions and generous help. This work was supported by the Brazilian agencies FINEP, CNPq, CAPES and BNB-FUNDECI.

Conflict of Interest

The authors declare no competing financial interest.

Supplementary material

11130_2015_500_MOESM1_ESM.pdf (120 kb)
ESM 1 (PDF 120 kb)

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Katya Anaya
    • 1
    • 3
  • Ana C. B. Cruz
    • 1
  • Dayse C. S. Cunha
    • 1
  • Sandra M. N. Monteiro
    • 2
  • Elizeu A. dos Santos
    • 1
  1. 1.Departamento de Bioquímica, Centro de BiociênciasUniversidade Federal do Rio Grande do NorteNatalBrazil
  2. 2.Departamento de Nutrição, Centro de Ciências da SaúdeUniversidade Federal do Rio Grande do NorteNatalBrazil
  3. 3.Faculdade de Ciências da Saúde do TrairiUniversidade Federal do Rio Grande do NorteSanta CruzBrazil

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