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Plant Foods for Human Nutrition

, Volume 70, Issue 2, pp 207–214 | Cite as

Evaluation of Phenolic Antioxidant Capacity in Grains of Modern and Old Durum Wheat Genotypes by the Novel QUENCHERABTS Approach

  • Maura N. Laus
  • Nilde A. Di Benedetto
  • Rossella Caporizzi
  • Damiana Tozzi
  • Mario Soccio
  • Luigia Giuzio
  • Pasquale De Vita
  • Zina Flagella
  • Donato Pastore
Original Paper

Abstract

The QUENCHERABTS (QUick, Easy, New, CHEap and Reproducible) approach for antioxidant capacity (AC) determination is based on the direct reaction of 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation with fine solid food particles. So, it may resemble the antioxidant action in foods or in human gastrointestinal trait. Here, the QUENCHER approach was used to study AC of durum wheat (Triticum durum Desf.) grains. Firstly, it was assessed which kind of antioxidants determines QUENCHER response. This has been performed by comparing AC measured by QUENCHERABTS and that measured by classical TEACABTS (Trolox equivalent antioxidant capacity) in four different extracts from whole flour of 10 durum wheat varieties containing: lipophilic, hydrophilic, insoluble-bound phenolic (IBP) and free-soluble phenolic (FSP) compounds. QUENCHERABTS data were unrelated to AC of water-extractable antioxidants and weakly correlated (r = 0.405, P < 0.05) to AC of the lipophilic ones; on the contrary, QUENCHERABTS response was mainly related to AC of IBP (r = 0.907, P < 0.001) and to a lesser extent of FSP extracts (r = 0.747, P < 0.001). Consistently, correlation was also found with the phenolic content of IBP and FSP (r = 0.760, P < 0.001 and r = 0.522, P < 0.01, respectively), thus confirming that QUENCHERABTS assay mainly assesses AC due to IBP. So, this assay was used in a first screening study to compare AC of bioactive IBP of thirty-six genotypes/landraces covering a century of cultivation in Italy. Interestingly, no relevant AC difference between modern and old genotypes was found, thus suggesting that a century of plant breeding did not decrease phenol-dependent health potential in durum wheat.

Keywords

Antioxidant capacity Durum wheat grains Phenolic compounds Old and modern genotypes QUENCHER assay TEAC assay 

Abbreviations

ABTS

2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)

AC

Antioxidant capacity

DPPH

2,2-diphenyl-1-picrylhydrazyl

d.w.

Dry weight

FSP

Free-soluble phenolic

FSPC

Free-soluble phenolic content

H

Hydrophilic

IBP

Insoluble-bound phenolic

IBPC

Insoluble-bound phenolic content

L

Lipophilic

PC

Phenolic content

QUENCHER

QUick, Easy, New, CHEap and Reproducible

TAC

Total antioxidant capacity

TEAC

Trolox equivalent antioxidant capacity

TPC

Total phenolic content

Notes

Acknowledgments

This work was supported by the following research projects: MiPAAF projects “GRANOBIO” and “FRUDUSAL”; MiUR project PON 01_01145 “ISCOCEM”. We thank Dr Carlo Robbe who participated as a student to the present work.

Conflict of Interest

The authors declare that they have no conflict of interest. This article does not contain any studies with human or animal subjects.

References

  1. 1.
    Pastore D, Laus MN, Tozzi D, Fogliano V, Soccio M, Flagella Z (2009) New tool to evaluate a comprehensive antioxidant activity in food extracts: bleaching of 4-nitroso-N, N-dimethylaniline catalyzed by soybean lipoxygenase-1. J Agric Food Chem 57:9682–9692CrossRefGoogle Scholar
  2. 2.
    Laus MN, Tozzi D, Soccio M, Fratianni A, Panfili G, Pastore D (2012) Dissection of antioxidant activity of durum wheat (Triticum durum Desf.) grains as evaluated by the new LOX/RNO method. J Cereal Sci 56:214–222CrossRefGoogle Scholar
  3. 3.
    Liu RH (2007) Whole grain phytochemicals and health. J Cereal Sci 46:207–219CrossRefGoogle Scholar
  4. 4.
    Laus MN, Gagliardi A, Soccio M, Flagella Z, Pastore D (2012) Antioxidant activity of free and bound compounds in quinoa (Chenopodium quinoa Willd.) seeds in comparison with durum wheat and emmer. J Food Sci 77:1150–1155CrossRefGoogle Scholar
  5. 5.
    Serpen A, Gokmen V, Pellegrini N, Fogliano V (2008) Direct measurement of the total antioxidant capacity of cereal products. J Cereal Sci 48:816–820CrossRefGoogle Scholar
  6. 6.
    Serpen A, Capuano E, Fogliano V, Gokmen V (2007) A new procedure to measure the antioxidant activity of insoluble food components. J Agric Food Chem 55:7676–7681CrossRefGoogle Scholar
  7. 7.
    Gokmen V, Serpen A, Fogliano V (2009) Direct measurement of the total antioxidant capacity of foods: the ‘QUENCHER’ approach. Trends Food Sci Technol 20:278–288CrossRefGoogle Scholar
  8. 8.
    Žilić S, Serpen A, Akıllıoglu G, Jankovic M, Gökmen V (2012) Distributions of phenolic compounds, yellow pigments and oxidative enzymes in wheat grains and their relation to antioxidant capacity of bran and debranned flour. J Cereal Sci 56:652–658CrossRefGoogle Scholar
  9. 9.
    Serpen A, Gokmen V, Fogliano V (2012) Solvent effects on total antioxidant capacity of foods measured by direct QUENCHER procedure. J Food Compos Anal 26:52–57CrossRefGoogle Scholar
  10. 10.
    Serpen A, Gokmen V (2009) Evaluation of the Maillard reaction in potato crisps by acrylamide, antioxidant capacity and color. J Food Compos Anal 22:589–595CrossRefGoogle Scholar
  11. 11.
    Ciesarova Z, Kukurova K, Bednarikova A, Morales FJ (2009) Effect of heat treatment and dough formulation on the formation of Maillard reaction products in fine bakery products-benefits and weak points. J Food Nutr Res 48:20–30Google Scholar
  12. 12.
    Serpen A, Gokmen V, Mogol BA (2012) Effects of different grain mixtures on Maillard reaction products and total antioxidant capacities of breads. J Food Compos Anal 26:160–168CrossRefGoogle Scholar
  13. 13.
    Acar OC, Gokmen V, Pellegrini N, Fogliano V (2009) Direct evaluation of the total antioxidant capacity of raw and roasted pulses, nuts and seeds. Eur Food Res Technol 229:961–969CrossRefGoogle Scholar
  14. 14.
    Rufian-Henares JA, Delgado-Andrade C (2009) Effect of digestive process on Maillard reaction indexes and antioxidant properties of breakfast cereals. Food Res Int 42:394–400CrossRefGoogle Scholar
  15. 15.
    Serpen A, Gokmen V, Fogliano V (2012) Total antioxidant capacities of raw and cooked meats. Meat Sci 90:60–65CrossRefGoogle Scholar
  16. 16.
    Žilić S, Akillioglu HG, Serpen A, Peric V, Gokmen V (2013) Comparison of phenolic compounds, isoflavones, antioxidant capacity and oxidative enzymes in yellow and black soybeans seed coat and dehulled bean. Eur Food Res Technol 237:409–418CrossRefGoogle Scholar
  17. 17.
    Amigo-Benavent M, del Castillo MD, Fogliano V (2010) Are the major antioxidants derived from soy protein and fructo-oligosaccharides model systems colored aqueous soluble or insoluble compounds? Eur Food Res Technol 231:545–553CrossRefGoogle Scholar
  18. 18.
    Tufan AN, Celik SE, Ozyurek M, Guclu K, Apak R (2013) Direct measurement of total antioxidant capacity of cereals: QUENCHER-CUPRAC method. Talanta 108:136–142CrossRefGoogle Scholar
  19. 19.
    Kraujalis P, Venskutonis PR, Kraujalienė V, Pukalskas A (2013) Antioxidant properties and preliminary evaluation of phytochemical composition of different anatomical parts of amaranth. Plant Foods Hum Nutr 68:322–328CrossRefGoogle Scholar
  20. 20.
    Dinelli G, Segura-Carretero A, Di Silvestro R, Marottia I, Arráez-Román D, Benedettelli S, Ghiselli L, Fernadez-Gutierrez A (2011) Profiles of phenolic compounds in modern and old common wheat varieties determined by liquid chromatography coupled with time-of-flight mass spectrometry. J Chromatogr A 1218:7670–7681CrossRefGoogle Scholar
  21. 21.
    Sosulski F, Krygier K, Hogge L (1982) Free, esterified, and insoluble-bound phenolic acids. 3. Composition of phenolic acids in cereal and potato flours. J Agric Food Chem 30:337–340CrossRefGoogle Scholar
  22. 22.
    Panfili G, Fratianni A, Irano M (2003) Normal phase high-performance liquid chromatography method for the determination of tocopherols and tocotrienols in cereal foods. J Agric Food Chem 51:3940–3944CrossRefGoogle Scholar
  23. 23.
    Singleton VL, Orthofer R, Lamuela-Raventos RM (1999) Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol 299:152–178CrossRefGoogle Scholar
  24. 24.
    Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26:1231–1237CrossRefGoogle Scholar
  25. 25.
    Zielinski H, Kozlowska H (2000) Antioxidant activity and total phenolics in selected cereal grains and their different morphological fractions. J Agric Food Chem 48:2008–2016CrossRefGoogle Scholar
  26. 26.
    Heimler D, Vignolini P, Isolani L, Arfaioli P, Ghiselli L, Romani A (2010) Polyphenol content of modern and old varieties of Triticum aestivum L. and T. durum Desf. grains in two years of production. J Agric Food Chem 58:7329–7334CrossRefGoogle Scholar
  27. 27.
    Shewry PR, Charmet G, Branlard G, Lafiandra D, Gergelyd S, Salgo A, Saulniere L, Bedo Z, Mills ENC, Ward JL (2012) Developing new types of wheat with enhanced health benefits. Trends Food Sci Technol 25:70–77CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Maura N. Laus
    • 1
  • Nilde A. Di Benedetto
    • 1
  • Rossella Caporizzi
    • 1
  • Damiana Tozzi
    • 1
  • Mario Soccio
    • 1
  • Luigia Giuzio
    • 1
  • Pasquale De Vita
    • 2
  • Zina Flagella
    • 1
  • Donato Pastore
    • 1
  1. 1.Dipartimento di Scienze Agrarie, degli Alimenti e dell’AmbienteUniversità degli Studi di FoggiaFoggiaItaly
  2. 2.Consiglio per la Ricerca e la sperimentazione in Agricoltura - Centro di Ricerca per la CerealicolturaFoggiaItaly

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