Plant Foods for Human Nutrition

, Volume 69, Issue 4, pp 337–343 | Cite as

Use of Different Spices as Potential Natural Antioxidant Additives on Cooked Beans (Phaseolus vulgaris). Increase of DPPH Radical Scavenging Activity and Total Phenolic Content

  • Marina Pelincer Pereira
  • Olga Luisa Tavano
Original Paper


Herbs and spices, excellent sources of phenolic compounds, can be considered potential antioxidant additives. The use of spices must strike a balance between their potential antioxidant capabilities during preparation and the flavor acceptance, in order to avoid rejection of the food. The aimed of this study is to evaluate the influence of different spices and their concentrations on cooked common beans, focusing its potential as antioxidant additives. Onion, parsley, spring onion, laurel and coriander increased the antioxidant activity of preparation when used at 7.96 g of onion, 1.06 g parsley, 3.43 g spring onion, 0.25 g laurel (dry leaves), and 0.43 g coriander/100 g of cooked beans. Besides, these spices concentrations enhance total phenolics and alter the mixture protein digestibility minimally. For garlic samples it was not possible to establish a concentration that increases the antioxidant activity of cooked beans.


Spice Phaseolus vulgaris Antioxidant DPPH Phenolic Flavonoids 



We would like to thank FAPEMIG for Marina P. Pereira fellowship. We feel grateful to Prof. Dr. Roseli A. da Silva Gomes for making available the resources of the Biochemical and Biophysical Laboratory/UFTM, and Ney Pereira for his help during the linguistic revision.

Conflict of interest

The authors declare that they have no conflict of interest and we would like to declare that FAPEMIG (Fundação de Amparo à Pesquisa do estado de Minas Gerais – Brazil) was responsible for the financial support of Marina P. Pereira (BIC/FAPEMIG Fellowship).


  1. 1.
    Vinson JA, Hao Y, Su X, Zubik L (1998) Phenol antioxidant quantity and quality in foods: vegetables. J Agric Food Chem 46:3630–3634CrossRefGoogle Scholar
  2. 2.
    Rice-Evans C, Miller N, Paganga G (1997) Antioxidant properties of phenolic compounds. Trends Plant Sci 2:152–159CrossRefGoogle Scholar
  3. 3.
    Biesalski HK, Dragsted LO, Elmadfa I et al (2009) Bioactive compounds: definition and assessment of activity. Nutr 25:1202–1205CrossRefGoogle Scholar
  4. 4.
    Espın JC, Garcıa-Conesa MT, Tomas-Barberan FA (2007) Nutraceuticals: facts and fiction. Phytochem 68:2986–3008CrossRefGoogle Scholar
  5. 5.
    Kris-Etherton PM, Hecker KD, Bonanome A, Coval SM, Binkoski AE, Hilpert KF, Griel AE, Etherton TD (2002) Bioactive compounds in foods: their role in the prevention of cardiovascular disease and cancer. Am J Med 113:71S–88SCrossRefGoogle Scholar
  6. 6.
    Halliwell B (1997) Antioxidants: the basics – what they are and how to evaluate them. Adv Pharmacol 38:3–20CrossRefGoogle Scholar
  7. 7.
    Niki E (2010) Assessment of antioxidant capacity in vitro and in vivo. Free Radic Biol Med 49:503–515CrossRefGoogle Scholar
  8. 8.
    Fraga CG (2007) Plant polyphenols: how to translate their in vitro antioxidant actions to in vivo conditions. IUBMB Life 59:308–315CrossRefGoogle Scholar
  9. 9.
    Boateng J, Verghese M, Walker LT, Ogutu S (2008) Effect of processing on antioxidant contents in selected dry beans (Phaseolus spp. L.). LWT Food Sci Technol 41:1541–1547Google Scholar
  10. 10.
    Granito M, Paolini M, Perez S (2008) Polyphenols and antioxidant capacity of Phaseolus vulgaris stored under extreme conditions and processed. LWT Food Sci Technol 41:994–999CrossRefGoogle Scholar
  11. 11.
    Kaefer CM, Milner JA (2008) The role of herbs and spices in cancer prevention. J Nutr Biochem 19:347–361CrossRefGoogle Scholar
  12. 12.
    Hinneburg I, Damien Dorman HJ, Hiltunen R (2006) Antioxidant activities of extracts from selected culinary herbs and spices. Food Chem 97:122–129CrossRefGoogle Scholar
  13. 13.
    Suhaj M (2006) Spice antioxidants isolation and their antiradical activity: a review. J Food Compos Anal 19:531–537CrossRefGoogle Scholar
  14. 14.
    Gawlik-Dziki U (2012) Dietary spices as natural effectors of lipoxygenase, xanthine oxidase, peroxidase and antioxidant agents. LWT Food Sci Technol 47:138–146CrossRefGoogle Scholar
  15. 15.
    Camargo EB, Botelho RA (2008) Técnica Dietética: Seleção e Preparo de Alimentos – Manual de Laboratório, 2nd edn. Atheneu, São PauloGoogle Scholar
  16. 16.
    Prakash VHP, Prakash J (1999) In vitro protein digestibility of legumes cooked with spices. Nahrung 43:19–21CrossRefGoogle Scholar
  17. 17.
    Brand-Williams W, Cuvelier ME, Berset C (1995) Use of free radical method to evaluate antioxidant activity. LWT Food Sci Technol 28:25–30CrossRefGoogle Scholar
  18. 18.
    Lu M, Yuan B, Zeng M, Chen J (2011) Antioxidant capacity and major phenolic compounds of spices commonly consumed in China. Food Res Int 44:530–536CrossRefGoogle Scholar
  19. 19.
    Akeson WR, Stahman MAA (1964) Pepsin-pancreatin digest index of protein quality evaluation. J Nutr 83:257–261Google Scholar
  20. 20.
    Church FCC, Swaisgood HE, Porter DH, Catignani GL (1983) Spectrophotometric assay using o-phthaldialdehyde for determination of proteolysis in milk and isolated milk proteins. J Dairy Sci 66:1219–1227CrossRefGoogle Scholar
  21. 21.
    Statistica 8.0. Statistics computer program for Windows, v.1. Tulsa: StatSoft Inc. Software, 2007Google Scholar
  22. 22.
    Balasundram N, Sundram K, Samman S (2006) Phenolic compounds in plants and agri-industrial by-products: antioxidant activity, occurrence and potential uses. Food Chem 99:191–203CrossRefGoogle Scholar
  23. 23.
    Yao LH, Jiang YM, Shi J, Tomas-Barber FA, Datta N, Singanusong R, Chen SS (2004) Flavonoids in food and their health benefits. Plant Foods Hum Nutr 59:113–122CrossRefGoogle Scholar
  24. 24.
    Wong PYY, Kitts DD (2006) Studies on the dual antioxidant and antibacterial properties of parsley (Petroselinum crispum) and cilantro (Coriandrum sativum) extracts. Food Chem 97:505–515CrossRefGoogle Scholar
  25. 25.
    Kaiser A, Kammerer DR, Carle R (2013) Impact of blanching on polyphenol stability and antioxidant capacity of innovative coriander (Coriandrum sativum L.) pastes. Food Chem 140:332–339CrossRefGoogle Scholar
  26. 26.
    Khatun M, Eguchi S, Yamaguchi T et al (2006) Effect of thermal treatment on radical-scavenging activity of some spices. Food Sci Technol Res 12:178–185CrossRefGoogle Scholar
  27. 27.
    Dewanto V, Wu X, Adom KK et al (2002) Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. J Agric Food Chem 50:3010–3014CrossRefGoogle Scholar
  28. 28.
    Özşen D, Erge HS (2013) Degradation kinetics of bioactive compounds and change in the antioxidant activity of wild strawberry (Fragaria vesca) pulp during heating. Food Bioprocess Technol 6:2261–2267CrossRefGoogle Scholar
  29. 29.
    Tomaino A, Cimino F, Zimbalatti V et al (2005) Influence of heating on antioxidant activity and the composition of some spice essential oils. Food Chem 89:549–554CrossRefGoogle Scholar
  30. 30.
    Dorta E, Lobo M, González M (2013) Improving the efficiency of antioxidant extraction from mango peel by using microwave-assisted extraction. Plant Foods Hum Nutr 68:190–199CrossRefGoogle Scholar
  31. 31.
    Singh U, Jambunathan R (1981) Studies on desi and kabuli chickpea (Cicer arietinum L.) cultivars: levels of protease inhibitors, levels of polyphenolic componds and in vitro protein digestibility. J Food Sci 46:1364–1367CrossRefGoogle Scholar
  32. 32.
    Friedman M (1996) Nutritional value of proteins from different food sources. A review. J Agric Food Chem 44:6–29CrossRefGoogle Scholar
  33. 33.
    Oste RE (1991) Digestibility of processed food protein. Nutritional and toxicological consequences of food processing. Adv Exp Med Biol 289:371–388CrossRefGoogle Scholar
  34. 34.
    Pradeep KU, Geervani P, Eggum BO (1991) Influence of spices on utilization of sorghum and chickpea protein. Plant Foods Hum Nutr 41:269–276Google Scholar

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Departament of Nutrition - Federal University of Triângulo MineiroUberabaBrazil
  2. 2.Faculty of Nutrition – Federal University of Alfenas – UNIFAL-MGAlfenasBrazil

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