Plant Foods for Human Nutrition

, Volume 68, Issue 3, pp 268–273 | Cite as

The Effects of the Decaffeination of Coffee Samples on Platelet Aggregation in Hyperlipidemic Rats

  • Alessandra dos Santos Danziger Silvério
  • Rosemary Gualberto Fonseca Alvarenga Pereira
  • Adriene Ribeiro Lima
  • Fernanda Borges de Araújo Paula
  • Maria Rita Rodrigues
  • Lineu BaldisseraJr.
  • Stella Maris da Silveira Duarte
Original Paper


The effect of coffee on cardiovascular diseases is still controversial. It is known that the process of decaffeination may influence the chemical constitution and, therefore, the biological effects of coffee. This study thus evaluated the effects of decaffeination on the levels of total phenols and chlorogenic acids in Coffea arabica L. samples, as well as the effects of ingesting both integral and decaffeinated coffee on the lipid profile and hemostatic and hematological parameters in normal and hyperlipidemic rats. Samples of integral and decaffeinated lyophilized coffee (Coffea arabica L., planted in Brazil) were used for chemical analysis (total phenols, chlorogenic acid and caffeine contents). For the bioassays, coffee beverages were prepared with non-lyophilized samples (10 % w/v) and were filtered and administered to animals by gavage (7.2 mL/kg/day) over 30 days. On the 31st day after beginning the treatment with coffee beverages, hyperlipidemia was induced to the animals by administering Triton WR-1339 (300 mg/kg body weight). On day 32, blood was taken to determine the lipid profile, platelet aggregation, prothrombin time, partially activated thromboplastin time and hemogram. The contents of both phenolic compounds and chlorogenic acid in the integral coffee beverage were significantly lower than those in the decaffeinated coffee beverage. The animals treated with Triton WR-1339 presented a mixed hyperlipidemia. Although the decaffeination process caused a relative increase in total phenols and chlorogenic acids, the coffee drinks were unable to change the lipid profile or the hemostatic and hematological parameters in the studied animals.


Decaffeination Coffee Platelets Aggregation Hyperlipidemia 



Coronary artery disease


Chlorogenic acids


Control animals


Animals treated with integral coffee


Animals treated with decaffeinated coffee


Animals treated with Triton WR 1339


Animals treated with Triton WR 1339 and integral coffee


Animals treated with Triton WR 1339 and decaffeinated coffee


Prothrombin time


Activated partial thromboplastin time


High density lipoprotein


System for analysis of variance


Aggregation platelet



The authors thank the FAPEMIG and CNPq.

Conflict of interest

The authors declare that they have no conflict of interest with any financial organization regarding the material discussed in the manuscript.


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Alessandra dos Santos Danziger Silvério
    • 2
  • Rosemary Gualberto Fonseca Alvarenga Pereira
    • 2
  • Adriene Ribeiro Lima
    • 2
  • Fernanda Borges de Araújo Paula
    • 1
  • Maria Rita Rodrigues
    • 1
  • Lineu BaldisseraJr.
    • 3
  • Stella Maris da Silveira Duarte
    • 1
  1. 1.Faculdade de Ciências FarmacêuticasUniversidade Federal de AlfenasAlfenasBrazil
  2. 2.Departamento de Ciência dos AlimentosUniversidade Federal de LavrasLavrasBrazil
  3. 3.Faculdade de Ciências Médicas, Departamento de FarmacologiaUniversidade Estadual de CampinasCampinasBrazil

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