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Plant Foods for Human Nutrition

, Volume 68, Issue 2, pp 190–199 | Cite as

Improving the Efficiency of Antioxidant Extraction from Mango Peel by Using Microwave-assisted Extraction

  • Eva Dorta
  • M. Gloria Lobo
  • Mónica González
Original Paper

Abstract

The purpose of this study was to analyze the extraction efficiency of antioxidants from mango peel by comparing two techniques: microwave-assisted (MAE) and traditional solvent (TE) extraction. The number of extraction steps, water content in the extractant, peel weight-to-solvent volume ratio in extractions and extraction time all had an influence on obtaining extracts with high antioxidant capacity, but the extraction technique and the water content in the extractant were the factors with the greatest effect. Using three steps, a water content of 50 % in the ethanol:water extractant, an extraction time of 60 min and a weight-to-volume ratio of 1:10 or 1:50 (w/v) led to the highest antioxidant activity and phytochemicals content in extracts. The extraction time needed to extract phytochemicals from mango peel was similar when MAE and TE were used. However, the antioxidant capacity and phytochemical content were around 1.5–6.0 times higher in the extracts obtained by MAE.

Keywords

Mangifera indica L. bio-wastes Microwave-assisted extraction Solvent extraction Antioxidant capacity Phenolic compounds 

Abbreviations

AAC

Antioxidant activity coefficient

\( \mathrm{ABT}{{\mathrm{S}}^{{\bullet +}}} \)

2,2′-Azino-bis-(3-ethylbenzothiazoline)-6-sulfonic acid radical

ANOVA

Analysis of variance

CCD

Central composite design

CF

Contribution factor

\( \mathrm{DPP}{{\mathrm{H}}^{\bullet }} \)

2,2-Diphenyl-1-picrylhydrazyl radical

DW

Dry matter basis

EV

Experimental values

LEs

Leucoanthocyanidin equivalents

MAE

Microwave-assisted extraction

MW

Microwave

PV

Predicted values

p

Probability

PVPP

Polyvinylpolypyrrolidone

SEE

Standard error of the estimates

TBARS

Thiobarbituric acid reactive substances

TAEs

Tannic acid equivalents

TE

Traditional solvent extraction

TEAC

Trolox equivalent antioxidant capacity

Notes

Acknowledgments

The Spanish INIA awarded Eva Dorta a PhD INIA grant. Mónica González would like to thank the Spanish National Research Council (CSIC) for a contract in the JAEdoc program, financed with the involvement of the European Social Fund (ESF). This research was supported through the RTA2006-00187 project, also financed by the INIA.

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

11130_2013_350_MOESM1_ESM.doc (40 kb)
ESM 1 (DOC 40 kb)

References

  1. 1.
    European Union (1995) Directive 1995/2/EC of the European Parliament and of the Council of 20 February 1995 on food additives other than colours and sweeteners. Off J Eur Comm L61Google Scholar
  2. 2.
    Schieber A, Berardini N, Carle R (2003) Identification of flavonol and xanthone glycosides from mango (Mangifera indica L. cv. “Tommy Atkins”) peels by high-performance liquid chromatography-electrospray ionization mass spectrometry. J Agric Food Chem 51:5006–5011CrossRefGoogle Scholar
  3. 3.
    Berardini N, Carle R, Schieber A (2004) Characterization of gallotannins and benzophenone derivatives from mango (Mangifera indica L. cv. Tommy Atkins) peels, pulp and kernels by high-performance liquid chromatography/electrospray ionization mass spectrometry. Rapid Commun Mass Spectrom 18:2208–2216CrossRefGoogle Scholar
  4. 4.
    Berardini N, Fezer R, Conrad J, Beifuss U, Carle R, Schieber A (2005) Screening of mango (Mangifera indica L.) cultivars for their contents of flavonol O- and xanthone C-glycosides, anthocyanins, and pectin. J Agric Food Chem 53:1563–1570CrossRefGoogle Scholar
  5. 5.
    Barreto JC, Trevisan MTS, Hull WE, Erben G, de Brito ES, Pfundstein B, Wurtele G, Spiegelhalder B, Owen RW (2008) Characterization and quantitation of polyphenolic compounds in bark, kernel, leaves and peel of mango (Mangifera indica L.). J Agric Food Chem 56:5599–5610CrossRefGoogle Scholar
  6. 6.
    Ribeiro SMR, Barbosa LCA, Queiroz JH, Knödler M, Schieber A (2008) Phenolic compounds and antioxidant capacity of Brazilian mango (Mangifera indica L.) varieties. Food Chem 110:620–626CrossRefGoogle Scholar
  7. 7.
    Dorta E, Lobo MG, González M (2011) Reutilization of mango by-products: study of the effect of extraction solvent and temperature on their antioxidant properties. J Food Sci 77:C80–C88CrossRefGoogle Scholar
  8. 8.
    González M, González V (2010) Sample preparation of tropical and subtropical fruit biowastes to determine antioxidant phytochemicals. Anal Methods UK 2:1842–1866CrossRefGoogle Scholar
  9. 9.
    Güçlü-Üstündağ Ö, Mazza G (2009) Effects of pressurized low polarity water extraction parameters on antioxidant properties and composition of cow cockle seed extracts. Plant Foods Hum Nutr 64:32–38CrossRefGoogle Scholar
  10. 10.
    Ghafoor K, AL-Juhaimi FY, Choi YH (2012) Supercritical fluid extraction of phenolic compounds and antioxidants from grape (Vitis labrusca B.) seeds. Plant Foods Hum Nutr 67:407–414CrossRefGoogle Scholar
  11. 11.
    Pan Y, Wang K, Huang S, Wang H, Mu X, He C, Ji X, Zhang J, Huang F (2008) Antioxidant activity of microwave assisted extract of longan (Dimocarpus Longan Lour.) peel. Food Chem 106:1264–1270CrossRefGoogle Scholar
  12. 12.
    Desai M, Parikh J, Parikh PA (2010) Extraction of natural products using microwaves as a heat source. Sep Purif Rev 39:1–32CrossRefGoogle Scholar
  13. 13.
    Routray W, Orsat V (2012) Microwave-assisted extraction of flavonoids: a review. Food Bioprocess Technol 5:409–424CrossRefGoogle Scholar
  14. 14.
    Pérez-Serradilla JA, Luque de Castro MD (2011) Microwave-assisted extraction of phenolic compounds from wine lees and spray-drying of the extract. Food Chem 124:1652–1659CrossRefGoogle Scholar
  15. 15.
    Dorta E, Lobo MG, González M (2013) Optimization of factors affecting extraction of antioxidants from mango seed. Food Bioprocess Technol 6:1067–1081Google Scholar
  16. 16.
    Jiang IY, He S, Pan YJ, Sun CR (2010) Bioassay-guided isolation and EPR-assisted antioxidant evaluation of two valuable compounds from mango peels. Food Chem 119:1285–1292CrossRefGoogle Scholar
  17. 17.
    Kim H, Moon JY, Kim H, Lee DS, Cho M, Choi HK, Kim YS, Mosaddik A, Cho SK (2010) Antioxidant and antiproliferative activities of mango (Mangifera indica L.) flesh and peel. Food Chem 121:429–436CrossRefGoogle Scholar
  18. 18.
    Brand-Williams W, Cuvelier ME, Berset C (1995) Use of a free radical method to evaluate antioxidant activity. LWT Food Sci Technol 28:25–30CrossRefGoogle Scholar
  19. 19.
    González-Montelongo R, Lobo MG, González M (2010) The effect of extraction temperature, time and number of steps on the antioxidant capacity of methanolic banana peel extracts. Sep Purif Technol 71:347–355CrossRefGoogle Scholar
  20. 20.
    Arnao MB, Cano A, Acosta M (2001) The hydrophilic and lipophilic contribution to total antioxidant activity. Food Chem 73:239–244CrossRefGoogle Scholar
  21. 21.
    Miller HE (1971) A simplified method for the evaluation of antioxidants. J Am Oil Chem Soc 48:91CrossRefGoogle Scholar
  22. 22.
    Zhu H, Wang Y, Liu Y, Xia Y, Tang T (2010) Analysis of flavonoids in Portulaca oleracea L. by UV-Vis spectrophotometry with comparative study on different extraction technologies. Food Anal Method 3:90–97CrossRefGoogle Scholar
  23. 23.
    Hemwimon S, Pavasant P, Shotipruk A (2007) Microwave-assisted extraction of antioxidative anthraquinones from roots of Morinda citrifolia. Sep Purif Technol 54:44–50CrossRefGoogle Scholar
  24. 24.
    Nepote V, Grosso NR, Guzmán CA (2005) Optimization of extraction of phenolic antioxidants from peanut skins. J Sci Food Agric 85:33–38CrossRefGoogle Scholar
  25. 25.
    Kaufmann B, Christen P (2002) Recent extraction techniques for natural products: microwave-assisted extraction and pressurised solvent extraction. Phytochem Anal 13:105–113CrossRefGoogle Scholar
  26. 26.
    Liazid A, Palma M, Brigui J, Barroso CB (2007) Investigation on phenolic compounds stability during microwave-assisted extraction. J Chromatogr A 1140:29–34CrossRefGoogle Scholar
  27. 27.
    Sun Y, Liao X, Wang Z, Hu X, Chen F (2007) Optimization of microwave-assisted extraction of anthocyanins in red raspberries and identification of anthocyanin of extracts using high-performance liquid chromatography-mass spectrometry. Eur Food Res Technol 225:511–523CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.Instituto Canario de Investigaciones AgrariasLa LagunaSpain
  2. 2.Instituto de Química Orgánica General (IQOG-CSIC)MadridSpain

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